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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (1): 61-66.doi: 10.3736/jcim20100112

• Original Experimental Research • Previous Articles     Next Articles

Effects of cycloartocarpin A and artocarpin extracted from Fructus Artocarpi Heterophylli on apoptosis of SMMC-7721 and SGC-7901 cells

YL Yang,AJ Hou,HQ Zhang,H Shen,QS Li,CC Zhang,GF Zhu   

  • Received:2009-08-25 Accepted:2009-11-10 Online:2010-01-20 Published:2010-01-15
  • Contact: GF Zhu


To investigate the effects of cycloartocarpin A (ACR-2) and artocarpin (ACR-3), monomeric compounds isolated from Fructus Artocarpi Heterophylli, on apoptosis of SMMC-7721 and SGC-7901 cell lines.

SMMC-7721 and SGC-7901 cells were routinely cultured, and divided into experiment group and control group. The SMMC-7721 cells were treated with different concentrations of ACR-2 (3.46×10 –3, 13.82×10 –3, 55.30×10 –3 mmol/L) and ACR-3 (6.88×10 –3, 27.52×10 –3, 110.09×10 –3 mmol/L), and the SGC-7901 cells were also treated with different concentrations of ACR-2 (8.06×10 –3, 32.26×10 –3, 129.03×10 –3 mmol/L) and ACR-3 (2.87×10 –3, 11.47×10 –3, 45.87×10 –3 mmol/L), with PBS (DMSO<0.1%) as control treatment. Cell apoptosis was measured by double labeled staining with Hoechst33342/propidium iodide (PI) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) and flow cytometry.

ACR-2 and ACR-3 could induce apoptosis of SMMC-7721 and SGC-7901 cells. Some of SMMC-7721 and SGC-7901 cells demonstrated typical apoptosis after being treated with ACR-2 and ACR-3. Hoechst33342/PI staining showed that cells were fraught with overlapping nuclei and nuclear debris or lobule, and the nuclear appeared light blue. TUNEL showed that cells permeated with overlapping nuclei and nuclear debris or lobule, and the nuclear appeared brown. Less apoptotic cells were observed in negative control group, and the nuclear appeared light blue. The apoptosis rates of SMMC-7721 and SGC-7901 cells in the ACR-2 and ACR-3 treated groups were significant higher than those in the control group (P<0.05, P<0.01).

ACR-2 and ACR-3 can induce apoptosis of SMMC-7721 and SGC-7901 cells.

Table 1

Concentrations of ACR-2 and ACR-3"

Cell n ACR-2 (mmol/L) ACR-3 (mmol/L)
SMMC-7721 cells 6 3.46×10–3 6.88×10–3
6 13.82×10–3 27.52×10–3
6 55.30×10–3 110.09×10–3
SGC-7901 cells 6 8.06×10–3 2.87×10–3
6 32.26×10–3 11.47×10–3
6 129.03×10–3 45.87×10–3

Table 2

Effects of ACR-2 and ACR-3 on apoptosis of SMMC-7721 cells after 48-hour treatment ($\bar{x}±s$, %)"

Group n Apoptosis rate
Fluorescence microscopy TUNEL method Flow cytometry
PBS (DMSO<0.1%) 6 0.35±0.02 0.51±0.06 0.40±0.01
3.46×10–3 mmol/L ACR-2 6 5.33±1.56* 5.59±1.27* 5.50±0.24**
13.82×10–3 mmol/L ACR-2 6 6.00±1.53* 8.76±1.23** 5.91±0.27**
55.30×10–3 mmol/L ACR-2 6 21.13±3.51** 22.20±2.15** 18.80±1.21**
6.88×10–3 mmol/L ACR-3 6 4.78±0.57** 10.56±2.78* 9.43±0.45**
27.52×10–3 mmol/L ACR-3 6 7.71±1.55* 8.02±1.63* 6.60±0.56**
110.09×10–3 mmol/L ACR-3 6 25.09±2.18** 27.05±2.63** 30.11±3.45**

Table 3

Effects of ACR-2 and ACR-3 on apoptosis of SGC-7901 cells after 48-hour treatment ($\bar{x}±s$, %)"

Group n Apoptosis rate
Fluorescence microscopy TUNEL method Flow cytometry
PBS (DMSO<0.1%) 6 0.38±0.04 0.23±0.08 0.20±0.02
8.06×10–3 mmol/L ACR-2 6 3.72±0.55** 7.37±1.13** 6.30±1.07*
32.26×10–3 mmol/L ACR-2 6 15.17±3.44* 18.49±2.63** 20.04±0.35**
129.03×10–3 mmol/L ACR-2 6 24.42±1.34** 27.92±1.21** 33.21±3.37**
2.87×10–3 mmol/L ACR-3 6 7.69±1.08** 13.17±1.99** 12.70±2.32*
11.47×10–3 mmol/L ACR-3 6 24.57±2.17** 19.96±3.60* 21.11±2.65**
45.87×10–3 mmol/L ACR-3 6 33.23±4.38** 34.78±1.42** 30.83±2.74**
[1] Editorial Committee of Humanistic Press. Dictionary of plants[M]. Taipei: Humanistic Press, 1980: 1722-1723
人文出版社委员会. 植物大辞典[M]. 台北: 人文出版社, 1980: 1722-1723
[2] Editorial Committee for Flora of China of Chinese Academy of Sciences. Flora of China[M]. Beijing: Science Press, 1998: 40, 55-56
中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京: 科学出版社, 1998: 40, 55-56
[3] Boonlaksiri C, Oonanant W, Kongsaeree P, Kittakoop P, Tanticharoen M, Thebtaranonth Y . An antimalarial stilbene from Artocarpus integer[J]. Phytochemistry, 2000,54(4):415-417
doi: 10.1016/S0031-9422(00)00074-1 pmid: 10897483
[4] Zhu GF, Yang YL, Hou AJ, Zhang HQ, Li QS, Zhang CC . Inhibitory effects of ACR-2 and ACR-3 on SMMC-7721 and SGC-7901 cell lines and activity of caspase-3[J]. Hunan Zhong Yi Yao Da Xue Xue Bao, 2007,27(5):24-27
朱国福, 杨延龙, 侯爱君, 张慧卿, 李秋爽, 张聪聪 . 波罗蜜属药用植物化合物对SMMC-7721、SGC-7901细胞增殖及Caspase-3酶活性的影响[J]. 湖南中医药大学学报, 2007,27(5):24-27
[5] Situ ZQ, Wu JZ. Cell culture[M]. Xi’an: The World Publishing Co, 2004: 189-201
司徒镇强, 吴军正 . 细胞培养[M]. 西安: 世界图书出版公司, 2004: 189-201
[6] Wang CJ, Ji XY . The adverse effects of chemotherapy drugs in tumor[J]. Xian Dai Zhong Liu Yi Xue, 2006,14(10):1321-1323
王传金, 纪秀英 . 肿瘤化疗药物的不良反应[J]. 现代肿瘤医学, 2006,14(10):1321-1323
[7] Zhou CF, Wu Y . The adverse effects of chemotherapy drugs[J]. Zhonghua Fu Bu Ji Bing Za Zhi, 2006,6(6):432-433
周长芳, 吴颖 . 化疗药物的不良反应[J]. 中华腹部疾病杂志, 2006,6(6):432-433
[8] Zhu XM, Liu P . Research of Chinese medicine in prevention and treatment of the side effects of chemotherapeutic drugs[J]. Jie Fang Jun Yao Xue Xue Bao, 2001,17(6):321-323
朱秀美, 刘屏 . 国内中药防治化疗药物毒副作用的研究概况[J]. 解放军药学学报, 2001,17(6):321-323
[9] Jiangsu New Medical College . Dictionary of traditional Chinese medicine[M]. Shanghai: Shanghai Scientific and Technical Publishers, 1977: 1713
江苏新医学院. 中药大辞典[M]. 上海: 上海科学技术出版社, 1977: 1713
[10] Patil AD, Freyer AJ, Killmer L, Offen P, Taylor PB, Votta BJ, Johnson RK . A new dimeric dihydrochalcone and a new prenylated flavone from the bud cover of Artocarpus altilis: potent inhibitors of cathepsin K[J]. J Nat Prod, 2002,65(4):624-627
doi: 10.1021/np0105634
[11] Lien TP, Ripperger H, Porzel A, Sung TV, Adam G . Constituents of Artocarpus tonkinensis[J]. Pharmazie, 1998,53(5):353
[12] Hakim EH , Asnizar, Yurnawilis, Aimi N, Kitajima M, Takayama H.Artoindesianin P, a new prenylated flavone with cytotoxic activity from Artocarpus lanceifolius[J]. Fitoterapia, 2002,73(7-8):668-673
doi: 10.1016/S0367-326X(02)00226-5
[13] Hakim EH , Asnizar, Yurnawilis, Aimi N, Kitajima M, Takayama H.Effects of Artocarpus heterophyllus and Asteracanthus longifolia on glucose tolerance in normal human subjects and in maturity-onset diabetic patients[J]. J Ethnopharmcol, 1991,31(3):277-282
doi: 10.1016/0378-8741(91)90012-3
[14] van Rees BP, Saukkonen K, Ristim ki A, Polkowski W, Tytgat GN, Drillenburg P, Offerhaus GJ . Cyclooxygenase-2 expression during carcinogenesis in the human stomach[J]. J Pathol, 2002,196(2):171-179
doi: 10.1002/path.1033 pmid: 11793368
[15] Konturek PC, Bielanski W, Konturek SJ, Hartwich A, Pierzchalski P, Gonciarz M, Marlicz K, Starzynska T, Zuchowicz M, Darasz Z , Götze JP, Rehfeld JF, Hahn EG.Progastrin and cyclooxygenase-2 in colorectal cancer[J]. Dig Dis Sci, 2002,47(9):1984-1991
doi: 10.1023/A:1019652224424 pmid: 12353842
[16] Su BN, Cuendet M, Hawthorne ME, Kardono LB, Riswan S, Fong HH, Mehta RG, Pezzuto JM, Kinghorn AD . Constituents of the bark and twigs of Artocarpus dadah with cyclooxygenase inhibitory activity[J]. J Nat Prod, 2002,65(2):163-169
doi: 10.1021/np010451c
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