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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (1): 86-89.doi: 10.3736/jcim20100116

• Original Experimental Research • Previous Articles     Next Articles

Comparative study of different therapeutic methods on autoimmune premature ovarian failure in mice

 Li Dong,Yuan Tan, Yu-jia Ping, Nan-sun Zhu   

  1. Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2009-09-09 Accepted:2009-11-10 Online:2010-01-20 Published:2010-01-15
  • Contact: Li Dong


To investigate the effects of different methods of treatment, including tonifying the kidney, activating blood and soothing the liver on mice with autoimmune premature ovarian failure (POF).

After preparing ovarian antigen with the ovarian tissue of female BALB/C mice, POF in female BALB/C mice was induced by immunization injection of the ovarian antigen on multiple subcutaneous sites and two posterior soles. The mice were treated with Liuwei Dihuang Pill, Shaofu Zhuyu Granule, Chaihu Shugan Pill respectively after the third immunization. Levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), prolactin (PRL), testosterone (T), anti-ovarian antibodies (AOAb) and anti-zona pellucida antibody (aZP) in sera were analyze by enzymelinked immunosorbent assay.

Compared with the normal group, the levels of serum FSH and LH were increased and the level of E2was decreased in the untreated group. AOAb and aZP positive rates in the untreated group were obviously higher than those in the normal group. Liuwei Dihuang Pill could significantly reduce the levels of FSH and LH and increase the level of E2as compared with the untreated group, but there were no significant differences in the AOAb and aZP positive rates. Shaofu Zhuyu Granule and Chaihu Shugan Pill could significantly reduce the positive rates of AOAb and aZP as compared with the untreated group, but no significant effect on the levels of FSH, LH and E2were observed. The drugs had no effects on contents of PRL and T in sera.

Tonifying the kidney method can recover the ovarian function in POF mice mainly by regulating the hormone levels. Activating blood and soothing the liver methods can improve the humoral immune function in POF mice.

Key words: Premature ovarian failure, Therapy, Tonify the kidney, Activating blood, Soothe the liver, Mice

Table 1

Contents of serum FSH, LH, E2, PRL and T in mice in different groups ($\bar{x}±s$)"

Group n FSH (IU/L) LH (IU/L) E2 (ng/L) PRL (μg/L) T (nmol/L)
Normal 20 1.66±0.30 1.06±0.23 39.55±2.83 21.47±3.31 1.41±0.35
Untreated 20 2.69±0.43* 1.90±0.38* 17.01±2.39* 24.19±2.19 1.34±0.33
LWDHP 20 1.87±0.47 0.96±0.24 24.93±5.01* 24.11±3.76 1.46±0.25
SFZYG 20 2.21±0.44 1.47±0.25 19.00±1.89 25.02±2.88 1.34±0.12
CHSGP 20 2.58±0.56 1.75±0.18 16.61±2.76 23.67±4.33 1.36±0.25

Table 2

Incidences of serum positive AOAb and aZP in mice in different groups"

Group n AOAb-positive rate aZP-positive rate
Normal 20 0 0
Untreated 19 84.2% (16/19)* 78.9% (15/19)*
LWDHP 17 82.3% (14/17) 76.5% (13/17)
SFZYG 20 45.0% (9/20) 35.0% (7/20)
CHSGP 20 50.0% (10/20) 40.0% (8/20)
[1] Orshan SA, Furniss KK, Forst C, Santoro N . The lived experience of premature ovarian failure[J]. J Obstet Gynecol Neonatal Nurs, 2001,30(2):202-208
doi: 10.1111/j.1552-6909.2001.tb01536.x pmid: 11308110
[2] Pasquali EA . The impact of premature menopause on women's experience of self[J]. J Holist Nurs, 1999,17(4):346-364
doi: 10.1177/089801019901700404 pmid: 10818847
[3] Yan J, Wang T . Study on premature ovary failure etiology and the treatment with combined traditional Chinese-Western medicine[J]. Tianjin Yao Xue, 2006,18(3):66-68
闫洁, 王涛 . 卵巢早衰的病因及中西医结合治疗的研究[J]. 天津药学, 2006,18(3):66-68
[4] Teng XX . Syndrome analysis and etiological factor survey of 122 patients with premature ovarian failure[J]. Zhongguo Zhong Yi Yao Xin Xi Za Zhi, 2008,15(4):18-20
doi: 10.3969/j.issn.1005-5304.2008.04.008
滕秀香 . 122例卵巢早衰患者中医证候分析及致病因素调查[J]. 中国中医药信息杂志, 2008,15(4):18-20
doi: 10.3969/j.issn.1005-5304.2008.04.008
[5] Lin JH, Yan JH, Lin QD, Jiang SL, Shen GF . Determination of cellular and humoral immunity in mice of autoimmune ovarian failure[J]. Zhonghua Fu Chan Ke Za Zhi, 1998,33(12):735-736
doi: 10.3760/j.issn:0529-567X.1998.12.010
林建华, 严隽鸿, 林其德, 姜守玲, 沈冠凤 . 自身免疫性卵巢功能衰退小鼠体液免疫和细胞免疫的测定[J]. 中华妇产科杂志, 1998,33(12):735-736
doi: 10.3760/j.issn:0529-567X.1998.12.010
[6] Dong L, Jiang L, Zhu NS . Effects of “Bushen Huoxue Decoction” on serum FSH, LH, E2 and PRL in patients with premature ovarian failure[J]. Shanghai Zhong Yi Yao Za Zhi, 2007,41(11):48-49
董莉, 姜琳, 朱南孙 . 补肾活血方对卵巢早衰患者血清FSH、LH、E2及PRL的影响[J]. 上海中医药杂志, 2007,41(11):48-49
[7] Dong L, Jiang L, Meng W, Zhu NS . Preventive and therapeutic effects of Bushen Huoxue Recipe on autoimmune premature ovarian failure in mice[J]. J Chin Integr Med, 2008,6(3):294-297
董莉, 姜琳, 孟炜, 朱南孙 . 补肾活血方对小鼠自身免疫性卵巢功能衰退的防治作用[J]. 中西医结合学报, 2008,6(3):294-297
[8] Shen W, Yu CQ . Treating premature ovarian failure by reinforcing kidney[J]. J Chin Integr Med, 2006,4(6):653-656
沈慰, 俞超芹 . 从肾论治卵巢早衰[J]. 中西医结合学报, 2006,4(6):653-656
[9] Cai LR, Li DJ, Sun XX, Wang MY . Experimental study on preventive and therapeutic effect of Bushen Huoxue Recipe on autoimmune premature ovarian failure model[J]. Zhongguo Zhong Xi Yi Jie He Zha Zhi, 2001,21(2):126-129
蔡立荣, 李大金, 孙晓溪, 王明雁 . 补肾活血方对小鼠实验性卵巢早衰防治作用的研究[J]. 中国中西医结合杂志, 2001,21(2):126-129
[10] Cheng BB, Zhu L, Li CZ, Huang QZ . Effect of Zuogui pill on reproductive endocrine of female yin-deficiency mice[J]. Xian Dai Zhong Xi Yi Jie He Zha Zhi, 2003,12(13):1362-1364
doi: 10.3969/j.issn.1008-8849.2003.13.008
程彬彬, 朱玲, 李长征, 黄泉智 . 左归丸对雌性阴虚小鼠模型生殖内分泌的影响[J]. 现代中西医结合杂志, 2003,12(13):1362-1364
doi: 10.3969/j.issn.1008-8849.2003.13.008
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