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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (1): 80-85.doi: 10.3736/jcim20100115

• Original Experimental Research • Previous Articles     Next Articles

Effects of a glucocorticoid on development of kidney deficiency syndrome in a rat model of asthma

 Pei Wanga, Li-ping Xua, Xi-hong Jinb,Jing Yanga,Ren-hui Liua,Xiu-juan Wanga   

  1. a School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
    b Department of Tuina, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2009-08-28 Accepted:2009-11-22 Online:2010-01-20 Published:2010-01-15
  • Contact: Ren-hui Liu ,Xiu-juan Wang E-mail:liurenhui995@163.com;wxj0517@sina.com

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Objective

To observe the effects of dexamethasone (DXM) on changes of syndrome and hypothalamic-pituitary-adrenal (HPA) axis of asthmatic rats at different stages of intervention (before withdrawal, during withdrawal and after withdrawal).
Methods

Asthma was induced in rats by inhalation of 1% ovalbumin. Rats in DXM group were treated with different concentrations of DXM by intra-abdominal injection. At the different stages of DXM treatment (before withdrawal, during withdrawal and after withdrawal), symptoms of the rats were observed to differentiate the syndromes according to yin-deficiency and yang-deficiency measurement standard. Content of serum corticosterone (CORT) was detected by enzyme-linked immunosorbent assay, and contents of plasma adrenocorticotropic hormone (ACTH) and bone Gla-protein (BGP), and content of corticotropin-releasing hormone (CRH) in hypothalamic homogenate were detected by radioimmunoassay.
Results

Rats in the DXM group were differentiated as yin-deficiency syndrome before the stage of hormone withdrawal. During the period of hormone withdrawal and after the withdrawal, syndromes of rats in the DXM group accorded with the standards of yin- and yang-deficiency. During the period of treatment with DXM, contents of CORT, ACTH and CRH were significantly decreased, but recovered after DXM withdrawal. BGP level in the DXM group was lower than those in the normal group and the model group during all the stages.
Conclusion

Asthmatic rats treated with DXM have different syndrome characteristics in different stages. Kidney deficiency will be caused by DXM treatment.

Key words: Asthma, Glucocorticoid, Syndrome, Hypothalamic-pituitary-adrenal axis, Bone Gla-protein, Rats

Table 1

Body weight in rats (x±s, g)"

Group Body weight
1st day 42nd day 49th day 63rd day 77th day 91st day
Normal 129.34±7.78 372.41±35.11 374.90±37.95 434.70±47.05 450.00±49.15 496.60±42.58
Model 129.04±8.64 368.19±29.52 370.11±34.60 425.22±29.24 448.11±37.26 484.89±20.88
DXM 128.16±9.75 297.04±26.61**△△ 266.24±22.16**△△ 271.53±27.41**△△ 285.53±32.45**△△ 357.16±39.56**△△

Table 2

Degree of yin-deficiency in rats (x±s)"

Group n Degree of yin-deficiency
Normal
42nd day (before withdrawal) 29 1.00±0.09
49th day (before withdrawal) 29 1.00±0.10
63rd day (during withdrawal) 20 1.00±0.11
77th day (during withdrawal) 20 1.00±0.11
91st day (after withdrawal) 10 1.00±0.09
Model
42nd day (before withdrawal) 27 0.99±0.08
49th day (before withdrawal) 27 0.99±0.09
63rd day (during withdrawal) 18 0.98±0.07
77th day (during withdrawal) 18 1.00±0.08
91st day (after withdrawal) 9 0.98±0.04
DXM
42nd day (before withdrawal) 25 0.80±0.07**△△
49th day (before withdrawal) 25 0.71±0.06**△△
63rd day (during withdrawal) 17 0.62±0.06**△△
77th day (during withdrawal) 17 0.63±0.07**△△
91st day (after withdrawal) 8 0.72±0.08**△△

Table 3

Level of exuberance or deficiency of yang in rats (x±s)"

Group n Level of exuberance or deficiency of yang
Normal
42nd day (before withdrawal) 29 1.00±0.05
49th day (before withdrawal) 29 1.00±0.03
63rd day (during withdrawal) 20 1.00±0.02
77th day (during withdrawal) 20 1.00±0.02
91st day (after withdrawal) 10 1.00±0.04
Model
42nd day (before withdrawal) 27 1.00±0.03
49th day (before withdrawal) 27 1.02±0.04*
63rd day (during withdrawal) 18 1.00±0.02
77th day (during withdrawal) 18 1.01±0.04
91st day (after withdrawal) 9 0.96±0.02*
DXM
42nd day (before withdrawal) 25 1.03±0.03*△△
49th day (before withdrawal) 25 1.01±0.04
63rd day (during withdrawal) 17 1.00±0.02
77th day (during withdrawal) 17 0.97±0.04**△△
91st day (after withdrawal) 8 0.95±0.02**

Table 4

Contents of CORT, ACTH, CRH and BGP before withdrawal (x±s)"

Group n CORT (ng/L) ACTH (ng/L) CRH (ng/mg prot) BGP (ng/mL)
Normal 1 9 2 304.60±628.44 62.75±17.45 5.77±2.01 5.22±2.14
Model 1 9 1 658.27±485.60 32.66±17.21** 4.20±1.87* 3.64±1.40
DXM 1 8 235.20±160.17**△△ 19.67±8.82**△△ 3.40±1.28** 0.65±0.62**△△

Table 5

Contents of CORT, ACTH, CRH and BGP during withdrawal (x±s)"

Group n CORT (ng/L) ACTH (ng/L) CRH (ng/mg prot) BGP (ng/mL)
Normal 2 10 2 432.88±378.96 65.33±31.01 5.05±1.62 2.63±0.96
Model 2 9 1 905.06±410.87** 38.41±11.72 4.15±1.39 2.50±1.20
DXM 2 9 355.25±239.28**△△ 39.62±14.93 3.04±1.37**△ 0.79±0.53 **△△

Table 6

Contents of CORT, ACTH, CRH and BGP after withdrawal (x±s)"

Group n CORT (ng/L) ACTH (ng/L) CRH (ng/mg prot) BGP (ng/mL)
Normal 3 10 2 627.22±508.46 62.96±33.48 5.39±1.40 4.58±2.19
Model 3 9 2 318.42±364.32 48.79±19.90 3.80±0.94** 3.21±1.34
DXM 3 8 2 211.88±400.01* 30.67±19.14**△ 3.67±1.09** 1.93±0.91*△△
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