Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (5): 570-574.doi: 10.3736/jcim20110517

• Original Experimental Research • Previous Articles     Next Articles

Antidiabetic and hypolipidemic activities of Dillenia indica extract in diabetic rats

Sunil Kumar, Vipin Kumar(), Om Prakash   

  1. Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra 136119, Haryana, India
  • Received:2011-01-24 Accepted:2011-02-16 Online:2011-05-20 Published:2011-05-15

Objective: The present study was carried out to investigate the antidiabetic and hypolipidemic activities of bioactive fraction (ethyl acetate fraction) of Dillenia indica methanolic extract in experimental diabetic Wistar rats.
Methods: Type-1 diabetes was induced by single intraperitoneal injection of streptozotocin (60 mg/kg body weight), and type-2 diabetes was induced by single intraperitoneal injection of streptozotocin (60 mg/kg body weight), 15 min after the intraperitoneal injection of 120 mg/kg nicotinamide. The rats were treated by administering graded oral doses of isolated ethyl acetate fraction of methanolic extract of D. indica (DIEE), 200 and 400 mg/kg body weight, respectively, for 21 d. The blood glucose level was estimated at weekly intervals by glucometer. Serum cholesterol, triglycerides and high-density lipoprotein cholesterol (HDL-C) were also evaluated in normal and diabetic rats by an autoanalyzer.
Results: In both experimental models, daily oral treatment with DIEE for 21 d resulted in a significant reduction in blood glucose (P<0.01), serum cholesterol (P<0.05) and triglycerides (P<0.05) levels whereas HDL-C level was found to be increased (P<0.05) as compared with the diabetic control group.
Conclusion: DIEE at 400 mg/kg body weight has prominent antidiabetic effect in experimental type-1 and type-2 diabetes models in rats. It may therefore be used as an alternative remedy for the treatment of diabetes mellitus.

Key words: Dilleniaceae, plant extracts, diabetes mellitus, experimental, hypoglycemic agents, antilipemic agents, rats

[1] Bastaki S . Diabetes mellitus and its treatment[J]. Int J Diabetes Metab, 2005,13(3):111-134
pmid: 5186843
[2] Wild S, Roglic G, Green A, Sicree R, King H . Global prevalence of diabetes: estimates for the year 2000 and projections for 2030[J]. Diabetes Care, 2004,27(5):1047-1053
doi: 10.2337/diacare.27.5.1047 pmid: 15111519
[3] Goodman LS, Gilman G. The pharmacological basis of therapeutics [M]. New York: Macmillan Publishing, 1975
[4] Kameswara Rao B, Giri R, Kesavulu MM, Apparao C . Effect of oral administration of bark extracts of Pterocarpus santalinus L. on blood glucose level in experimental animals[J]. J Ethnopharmacol, 2001,74(1):69-74
doi: 10.1016/S0378-8741(00)00344-5
[5] Valiathan MS . Healing Plants[J]. Curr Sci, 1998,75(11):1122-1126
[6] Kirtikar KR, Basu BD . Indian medicinal plants. Vol 1[M]. Dehradun: Oriental Enterprizes, 2001,( 2nd ed):75-77
[7] Sharma HK, Chhangte L, Dolui AK . Traditional medicinal plants in Mizoram, India[J]. Fitoterapia, 2001,72(2):146-161
doi: 10.1016/S0367-326X(00)00278-1 pmid: 11223224
[8] Abdille MH, Singh RP, Jayaprakasha GK, Jena BS . Antioxidant activity of the extracts from Dillenia indica fruits[J]. Food Chem, 2005,90(4):891-896
doi: 10.1016/j.foodchem.2004.09.002
[9] Parvin MN, Rahman MS, Islam MS, Rashid MA . Chemical and biological investigations of Dillenia indica Linn[J]. Bangladesh J Pharmacol, 2009,4(2):122-125
doi: 10.3329/bjp.v4i2.2758
[10] Bhakuni DS, Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN . Screening of Indian plants for biological activity. Ⅱ[J]. Indian J Exp Biol, 1969,7(4):250-262
[11] Yeshwante SB, Juvekar AR, Nagmoti DM, Wankhede SS, Shah AS, Pimprikar RB, Saindane DS . Anti-inflammatory activity of methanolic extracts of Dillenia indica L. leaves[J]. J Young Pharm, 2009,1(1):63-66
doi: 10.4103/0975-1483.51885
[12] Apu AS, Muhit MS, Tareq SM, Pathan AH, Jamaluddin ATM, Ahmed M . Antimicrobial activity and brine shrimp lethality bioassay of the leaves extract of Dillenia indica linn[J]. J Young Pharm, 2010,2(1):50-53
doi: 10.4103/0975-1483.62213
[13] Himakar RK, Tharanath V, Nagi Reddy KB, Sharma PVGK, Reddy OVS . Studies on hepatoprotective effect of hexane extract of Dillenia indica against CCl4 induced toxicity and its safety evaluation in Wistar albino rats[J]. Res J Pharm Biol Chem Sci, 2010,1(3):441-450
[14] Muhit MA, Tareq SM, Apu AS, Basak D, Islam SM . Isolation and identification of compounds from the leaf extract of Dillenia indica Linn[J]. Bangladesh Pharm J, 2010,13(1):49-53
[15] Bate-Smith EC, Harborne JB . Differences in flavonoid content between fresh and herbarium leaf tissue in Dillenia[J]. Phytochemistry, 1971,10(5):1055-1058
doi: 10.1016/S0031-9422(00)89938-0
[16] Kumar D, Mallick S, Vedasiromoni JR, Pal BC . Anti-leukemic activity of Dillenia indica L. fruit extract and quantification of betulinic acid by HPLC[J]. Phytomedicine, 2010,17(6):431-435
doi: 10.1016/j.phymed.2009.07.010
[17] Sood SK, Bhardwaj R, Lakhanpal TN . Ethnic Indian plants in cure of diabetes[M]. Jodhpur: Scientific Publishers Journals Dept, 2005: 59
[18] Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, Novelli M, Ribes G . Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide[J]. Diabetes, 1998,47(2):224-229
doi: 10.2337/diab.47.2.224
[19] Rifai N, Bachorik PS, Albers JJ. Lipids, lipoproteins and apolipoproteins. In: Burtis CA, Ashwood ER. Tietz textbook of clinical chemistry [M]. Philadelphia: W.B. Saunders Company, 1999,( 3rd ed):809-861
[20] Burstein M, Scholnick HR, Morfin R . Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions[J]. J Lipid Res, 1970,11(6):583-595
[21] Junod A, Lambert AE, Stauffacher W, Renold AE . Diabetogenic action of streptozotocin: relationship of dose to metabolic response[J]. J Clin Invest, 1969,48(11):2129-2139
doi: 10.1172/JCI106180 pmid: 4241908
[22] Nagappa AN, Thakurdesai PA, Venkat Rao N, Singh J . Antidiabetic activity of Terminalia catappa Linn fruits[J]. J Ethnopharamacol, 2003,88(1):45-50
doi: 10.1016/S0378-8741(03)00208-3 pmid: 12902049
[23] Shanmugasundaram ER, Gopinath KL, Radha Shanmugasundaram K, Rajendran VM . Possible regeneration of the islets of Langerhans in streptozotocin-diabetic rats given Gymnema sylvestre leaf extracts[J]. J Ethnopharamacol, 1990,30(3):265-279
doi: 10.1016/0378-8741(90)90106-4
[24] Chi MS, Koh ET, Stewart TJ . Effects of garlic on lipid metabolism in rats fed cholesterol or lard[J]. J Nutr, 1982,112(2):241-248
doi: 10.1093/jn/112.2.241 pmid: 7057262
[1] Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[2] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[3] Bérénice Colleville, Nicolas Perzo, Guillaume Avinée, Anaïs Dumesnil, Frederic Ziegler, Paul Billoir, Hélène Eltchaninoff, Vincent Richard, Eric Durand. Impact of high-fat diet and vitamin D3 supplementation on aortic stenosis establishment in waved-2 epidermal growth factor receptor mutant mice. Journal of Integrative Medicine, 2019, 17(2): 107-114.
[4] Rohitash Jamwal. Bioavailable curcumin formulations: A review of pharmacokinetic studies in healthy volunteers. Journal of Integrative Medicine, 2018, 16(6): 367-374.
[5] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Thongchai Koobkokkruad, Chutichot Pattamadilok, Nontima Vardhanabhuti . Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2018, 16(6): 427-434.
[6] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[7] Shohini Chakraborty, Nashra Afaq, Neelam Singh, Sukanta Majumdar. Antimicrobial activity of Cannabis sativaThuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus. Journal of Integrative Medicine, 2018, 16(5): 350-357.
[8] Bruno José Martins Da Silva, Amanda Anastácia Pinto Hage, Edilene Oliveira Silva, Ana Paula Drummond Rodrigues. Medicinal plants from the Brazilian Amazonian region and their antileishmanial activity: A review. Journal of Integrative Medicine, 2018, 16(4): 211-222.
[9] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[10] Olakunle Bamikole Afolabi, Omotade Ibidun Oloyede, Shadrack Oludare Agunbiade. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. Journal of Integrative Medicine, 2018, 16(3): 192-198.
[11] Chun-song Hu, Tengiz Tkebuchava. SEEDi1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[12] Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[13] Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[14] Nisha Panth, Keshav Raj Paudel, Rajendra Karki. Phytochemical profile and biological activity of Juglans regia. Journal of Integrative Medicine, 2016, 14(5): 359-373.
[15] Musa Toyin Yakubu, Abdulquadri Funmbi Olutoye. Aphrodisiac activity of aqueous extract of Anthonotha macrophylla P. Beauv. leaves in female Wistar rats. Journal of Integrative Medicine, 2016, 14(5): 400-408.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[2] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[3] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[4] Chan Chen, Yong-mei Meng , Peng Zhang, Juan Wang, Hui-hui Zhao, Shu-zhen Guo , Wei Wang. Diagnosis and treatment rule of traditional Chinese medicine for syndrome factors of chronic congestive heart failure: a study based on Shannon entropy method. Journal of Chinese Integrative Medicine, 2010, 8(11): 1080-1084
[5] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[6] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[7] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[8] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[9] Ming-qing Wei , Jin-zhou Tian , Jing Shi , Fu-yun Ma , Ying-chun Miao , Yong-yan Wang. Effects of Chinese medicine for promoting blood circulation and removing blood stasis in treating patients with mild to moderate vascular dementia: a randomized, double-blind and parallel-controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1240-1246
[10] Nian-hong Wang , Jun-tao Yan , Wu-quan Sun , Yong-shan Hu , Jun Xia , Li-cheng Wei , Jie Jia , Gui-lin Ouyang , Yong He , Yan-ming Guo , Jie Xu . Effects of early application of Tuina treatment on quadriceps surface myoelectricity in patients after total knee arthroplasty: a randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1247-1253