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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (5): 570-574.doi: 10.3736/jcim20110517

• Original Experimental Research • Previous Articles     Next Articles

Antidiabetic and hypolipidemic activities of Dillenia indica extract in diabetic rats

Sunil Kumar, Vipin Kumar(), Om Prakash   

  1. Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra 136119, Haryana, India
  • Received:2011-01-24 Accepted:2011-02-16 Online:2011-05-20 Published:2011-05-15

Objective: The present study was carried out to investigate the antidiabetic and hypolipidemic activities of bioactive fraction (ethyl acetate fraction) of Dillenia indica methanolic extract in experimental diabetic Wistar rats.
Methods: Type-1 diabetes was induced by single intraperitoneal injection of streptozotocin (60 mg/kg body weight), and type-2 diabetes was induced by single intraperitoneal injection of streptozotocin (60 mg/kg body weight), 15 min after the intraperitoneal injection of 120 mg/kg nicotinamide. The rats were treated by administering graded oral doses of isolated ethyl acetate fraction of methanolic extract of D. indica (DIEE), 200 and 400 mg/kg body weight, respectively, for 21 d. The blood glucose level was estimated at weekly intervals by glucometer. Serum cholesterol, triglycerides and high-density lipoprotein cholesterol (HDL-C) were also evaluated in normal and diabetic rats by an autoanalyzer.
Results: In both experimental models, daily oral treatment with DIEE for 21 d resulted in a significant reduction in blood glucose (P<0.01), serum cholesterol (P<0.05) and triglycerides (P<0.05) levels whereas HDL-C level was found to be increased (P<0.05) as compared with the diabetic control group.
Conclusion: DIEE at 400 mg/kg body weight has prominent antidiabetic effect in experimental type-1 and type-2 diabetes models in rats. It may therefore be used as an alternative remedy for the treatment of diabetes mellitus.

Key words: Dilleniaceae, plant extracts, diabetes mellitus, experimental, hypoglycemic agents, antilipemic agents, rats

[1] Bastaki S . Diabetes mellitus and its treatment[J]. Int J Diabetes Metab, 2005,13(3):111-134
pmid: 5186843
[2] Wild S, Roglic G, Green A, Sicree R, King H . Global prevalence of diabetes: estimates for the year 2000 and projections for 2030[J]. Diabetes Care, 2004,27(5):1047-1053
doi: 10.2337/diacare.27.5.1047 pmid: 15111519
[3] Goodman LS, Gilman G. The pharmacological basis of therapeutics [M]. New York: Macmillan Publishing, 1975
[4] Kameswara Rao B, Giri R, Kesavulu MM, Apparao C . Effect of oral administration of bark extracts of Pterocarpus santalinus L. on blood glucose level in experimental animals[J]. J Ethnopharmacol, 2001,74(1):69-74
doi: 10.1016/S0378-8741(00)00344-5
[5] Valiathan MS . Healing Plants[J]. Curr Sci, 1998,75(11):1122-1126
[6] Kirtikar KR, Basu BD . Indian medicinal plants. Vol 1[M]. Dehradun: Oriental Enterprizes, 2001,( 2nd ed):75-77
[7] Sharma HK, Chhangte L, Dolui AK . Traditional medicinal plants in Mizoram, India[J]. Fitoterapia, 2001,72(2):146-161
doi: 10.1016/S0367-326X(00)00278-1 pmid: 11223224
[8] Abdille MH, Singh RP, Jayaprakasha GK, Jena BS . Antioxidant activity of the extracts from Dillenia indica fruits[J]. Food Chem, 2005,90(4):891-896
doi: 10.1016/j.foodchem.2004.09.002
[9] Parvin MN, Rahman MS, Islam MS, Rashid MA . Chemical and biological investigations of Dillenia indica Linn[J]. Bangladesh J Pharmacol, 2009,4(2):122-125
doi: 10.3329/bjp.v4i2.2758
[10] Bhakuni DS, Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN . Screening of Indian plants for biological activity. Ⅱ[J]. Indian J Exp Biol, 1969,7(4):250-262
[11] Yeshwante SB, Juvekar AR, Nagmoti DM, Wankhede SS, Shah AS, Pimprikar RB, Saindane DS . Anti-inflammatory activity of methanolic extracts of Dillenia indica L. leaves[J]. J Young Pharm, 2009,1(1):63-66
doi: 10.4103/0975-1483.51885
[12] Apu AS, Muhit MS, Tareq SM, Pathan AH, Jamaluddin ATM, Ahmed M . Antimicrobial activity and brine shrimp lethality bioassay of the leaves extract of Dillenia indica linn[J]. J Young Pharm, 2010,2(1):50-53
doi: 10.4103/0975-1483.62213
[13] Himakar RK, Tharanath V, Nagi Reddy KB, Sharma PVGK, Reddy OVS . Studies on hepatoprotective effect of hexane extract of Dillenia indica against CCl4 induced toxicity and its safety evaluation in Wistar albino rats[J]. Res J Pharm Biol Chem Sci, 2010,1(3):441-450
[14] Muhit MA, Tareq SM, Apu AS, Basak D, Islam SM . Isolation and identification of compounds from the leaf extract of Dillenia indica Linn[J]. Bangladesh Pharm J, 2010,13(1):49-53
[15] Bate-Smith EC, Harborne JB . Differences in flavonoid content between fresh and herbarium leaf tissue in Dillenia[J]. Phytochemistry, 1971,10(5):1055-1058
doi: 10.1016/S0031-9422(00)89938-0
[16] Kumar D, Mallick S, Vedasiromoni JR, Pal BC . Anti-leukemic activity of Dillenia indica L. fruit extract and quantification of betulinic acid by HPLC[J]. Phytomedicine, 2010,17(6):431-435
doi: 10.1016/j.phymed.2009.07.010
[17] Sood SK, Bhardwaj R, Lakhanpal TN . Ethnic Indian plants in cure of diabetes[M]. Jodhpur: Scientific Publishers Journals Dept, 2005: 59
[18] Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, Novelli M, Ribes G . Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide[J]. Diabetes, 1998,47(2):224-229
doi: 10.2337/diab.47.2.224
[19] Rifai N, Bachorik PS, Albers JJ. Lipids, lipoproteins and apolipoproteins. In: Burtis CA, Ashwood ER. Tietz textbook of clinical chemistry [M]. Philadelphia: W.B. Saunders Company, 1999,( 3rd ed):809-861
[20] Burstein M, Scholnick HR, Morfin R . Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions[J]. J Lipid Res, 1970,11(6):583-595
[21] Junod A, Lambert AE, Stauffacher W, Renold AE . Diabetogenic action of streptozotocin: relationship of dose to metabolic response[J]. J Clin Invest, 1969,48(11):2129-2139
doi: 10.1172/JCI106180 pmid: 4241908
[22] Nagappa AN, Thakurdesai PA, Venkat Rao N, Singh J . Antidiabetic activity of Terminalia catappa Linn fruits[J]. J Ethnopharamacol, 2003,88(1):45-50
doi: 10.1016/S0378-8741(03)00208-3 pmid: 12902049
[23] Shanmugasundaram ER, Gopinath KL, Radha Shanmugasundaram K, Rajendran VM . Possible regeneration of the islets of Langerhans in streptozotocin-diabetic rats given Gymnema sylvestre leaf extracts[J]. J Ethnopharamacol, 1990,30(3):265-279
doi: 10.1016/0378-8741(90)90106-4
[24] Chi MS, Koh ET, Stewart TJ . Effects of garlic on lipid metabolism in rats fed cholesterol or lard[J]. J Nutr, 1982,112(2):241-248
doi: 10.1093/jn/112.2.241 pmid: 7057262
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