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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (3): 205-208.doi: 10.3736/jcim20030318

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Effects of Yifei Kangliu Oral Liquid on cell cycle and protein-nucleic acid synthesis of experimental lung cancer

Ming-quan Han1(), Jia-xiang Liu2, Hong Gao2, Shan-xiang Chen2, Yan-wei Zhu2, Ling Xu3   

  1. 1. Department of Integrated Traditional Chinese and Western Medicine,Pulmonary Disease Hospital of Shanghai, Shanghai 200433, China
    2. Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032,China
    3. Department of Traditional Chinese Medicine, Changzheng Hospital, Shanghai 200003, China
  • Received:2003-07-21 Online:2003-09-20 Published:2003-09-20


To explore the effect of the traditional Chinese medicine Yifei Kangliu (YFKL) Oral Liquid on the proliferation, cell cycle and protein-nucleic acid synthesis of murine Lewis lung cancer cell and human lung adenocarcinoma cell line SPC-A-1.


The inhibiting rates of tumor growth were calculated by weighing the weight of tumor inoculated in vivo, combined by counting cancer cells in vitro. The ratio of the cell cycle and exponents of DNA, RNA, and protein were measured by flow cytometry (FCM).


The inhibiting rate of tumor growth in the treated group with YFKL Oral Liquid was 30.38% (P<0.05). The proportion of cells in S phase of the treated groups with YFKL Oral Liquid was lower than that of the control group. In the group with most significant result, 72% of the cells were stagnated in G0/G1 phase. The inhibiting rates of DNA, RNA and protein in murine Lewis lung cancer were 7.4%, 23.73% and 23.31% respectively. In SPC-A-1 cell line, the inhibiting rates were 9.3%, 10.1% and 14.7% respectively, demonstrating amplified effects on lower levels.


YFKL Oral Liquid significantly inhibited the proliferation of murine Lewis lung cancer cell and human lung adenocarcinoma cell line SPC-A-1 by blocking the cancer cells entering the proliferative phase resulted from its inhibition of DNA.

Key words: Yifei Kangliu Oral Liquid, lung neoplasms, flow cytometry, cell cycle, nucleic acid, protein

CLC Number: 

  • R734.2


组别 n 瘤重($\bar{x}$±s, g) 瘤重抑制率(%)
益肺抗瘤饮1组 8 1.71±0.49 19.98
益肺抗瘤饮2组 8 1.76±0.55 22.97
益肺抗瘤饮3组 8 1.49±0.52* 30.38
空白荷瘤对照组 8 2.14±0.69



组别 n G0/G1(%) S(%) G2 /M(%) PI(%)
益肺抗瘤饮1组 8 66.4** 31.5 3.1* 34.8*
益肺抗瘤饮2组 8 70.4** 24.7** 4.9* 29.6**
益肺抗瘤饮3组 8 72.6** 26.8** 0.6** 27.4**
空白荷瘤对照组 8 64.1 30.9 5.0 35.9


组别 n G0/G1(%) S(%) G2 /M(%) PI(%)
益肺抗瘤饮Ⅰ组 10 70.6** 24.0** 5.3* 29.3 **
益肺抗瘤饮Ⅱ组 10 70.8** 24.8** 4.4 29.2 **
对照组 10 64.1 33.6 2.3 35.9


组 别 n DI RI PrI
益肺抗瘤饮1组 8 1.4520±0.0374 1.5147±0.0229 0.7529±0.0043**
益肺抗瘤饮2组 8 1.4207±0.0333 1.4271±0.0274 0.7277±0.0042**
益肺抗瘤饮3组 8 1.4010±0.0077** 1.3367±0.0184* 0.6941±0.0049**
空白荷瘤对照组 8 1.5140±0.0252 1.5740±0.0415 0.9051±0.0133


组 别 n DI RI PrI
益肺抗瘤饮Ⅰ组 10 1.3050±0.0163** 1.4336±0.0123** 1.0113±0.0473
益肺抗瘤饮Ⅱ组 10 1.3430±0.0597 1.4102±0.0139** 0.9639±0.0165**
对照组 10 1.4810±0.0422 1.5693±0.0377 1.1309±0.0427
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