黄芪和三七主要有效成分配伍对氧化损伤所致的PC12细胞凋亡及其活性氧、线粒体膜电位的影响

doi:10.3736/jcim20121009

Abstract

Objective: To explore the effects and mechanisms of combining astragaloside Ⅳ (the effective component of Astragalus membranaceus) with notoginsenoside R1, ginsenoside Rb1 and ginsenoside Rg1 (the effective components of Panax notoginseng) against oxidative injury in PC12 cells induced by cobalt chloride (CoCl₂). .

Methods: CoCl₂ was used to stimulate PC12 cells to induce injury after transdifferentiation with nerve growth factor. Then the PC12 cells were divided into 10 groups and cultured with corresponding drugs. After culture, apoptotic cells were tested by using Hocchst 33258 fluorescent staining, the level of mitochondrial membrane potential (MMP) was analyzed by rhodamine 123 fluorescent staining and the content of reactive oxygen species (ROS) in PC12 cell was measured by dichlorofluorescin diacetate fluorescent staining.

Results: CoCl₂ induced apoptosis along with the obvious decrease of MMP as well as overproduction of ROS in PC12 cells. Astragaloside Ⅳ, ginsenosides Rg1, ginsenosides Rb1 and notoginsenoside R1 had inhibition effects in different degree on PC12 cell apoptosis induced by CoCl₂, reduced the overproduction of ROS and the decrease of MMP. The effects of the combination were better than those of active component alone.

Conclusion: Active components extracted from Astragalus and Panax notoginseng can inhibit PC12 cell apoptosis induced by oxidative injury, furthermore, the effects were enhanced by combination of these components, which may be associated with jointly antagonizing the generation of ROS and raising MMP.

Key words: plant extracts, compatibility (TCD), apoptosis, reactive oxygen species, mitochondrial membrane potential, PC12 cells

Xiao-ping Huang , Xiao-dan Liu , Chang-qing Deng . Effects of the combination of active component extracts from Astragalus membranaceus and Panax notoginseng on apoptosis, reactive oxygen species and mitochondrial membrane potential of PC12 cells with oxidative injury. Journal of Chinese Integrative Medicine, 2012, 10(10): 1127-1134.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20121009

http://www.jcimjournal.com/EN/Y2012/V10/I10/1127

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References 20

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Group	n	Apoptosis rate (%)	ROS content (MFI)	MMP level (MFI)
Blank	3	1.57±0.26	26.75±0.61	81.43±4.16
Ethanol-PBS	3	30.03±10.33^**	39.44±1.54^*	79.47±2.89
CoCl₂	3	54.17±1.60^**	55.54±2.08^**	44.56±5.56^**
CoCl₂-ethanol-PBS	3	89.83±6.26^△△▲▲	73.37±9.06^△△▲▲	30.70±1.92^△△▲
Astragaloside Ⅳ	3	37.94±4.37^□□■■	45.10±0.76^□□■■	31.68±0.62^■■
Ginsenosides Rg1	3	11.47±1.13^▲▲■	38.29±3.04^▲▲■	45.24±1.53^■■
Ginsenosides Rb1	3	16.66±3.13^▲▲■■	35.35±0.95^▲▲	49.57±2.74^■■
Notoginsenoside R1	3	13.00±1.52^▲▲■■	32.17±2.91^▲▲	48.47±4.31^■■
Combination	3	5.53±0.92^□□	34.05±1.35^□□	56.65±3.36^□□
Edaravone	3	16.30±0.65^▲▲■■	28.90±0.47^▲▲■■	47.44±2.13^■■

Effects of the combination of active component extracts from Astragalus membranaceus and Panax notoginseng on apoptosis, reactive oxygen species and mitochondrial membrane potential of PC12 cells with oxidative injury

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