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Journal of Integrative Medicine ›› 2018, Vol. 16 ›› Issue (6): 404-410.doi: 10.1016/j.joim.2018.08.004

• Original Experimental Research • Previous Articles     Next Articles

In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum

Bruno José Martins Da Silva a, b, 1, Sandro Wilson Gomes Pereira a, 1, Ana Paula Drummond Rodrigues c, José Luiz Martins Do Nascimento d, e, Edilene Oliveira Silva a, b   

  1. a Laboratory of Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará 66075-110, Brazil
    b National Institute of Science and Technology in Structural Biology and Bioimaging (INCT-INBEB), Rio de Janeiro 21941-901, Brazil
    c Laboratory of Electron Microscopy, Department of Health Surveillance, Ministry of Health, Evandro Chagas Institute, Belém, Pará 66087-082, Brazil
    d Laboratory of Molecular and Cellular Neurochemistry, Institute of Biological Sciences, Federal University of Pará, Belém, Pará 66075-110, Brazil
    e National Institute of Science and Technology in Neuroimmunomodulation (INCT-NIM), Rio de Janeiro 21040-360, Brazil
  • Received:2018-02-27 Accepted:2018-05-14 Online:2018-11-08 Published:2018-08-25
  • Contact: Edilene Oliveira Silva; E-mail: edilene@ufpa.br

Objective
In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root (AEPa) on Leishmania infantum proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages.


Methods

L. infantum promastigotes were treated with 50 and 100?μg/mL AEPa for 72?h and then antipromastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment.


Results

Treatment with AEPa reduced the numbers of L. infantum promastigotes (50% inhibitory concentration (IC50)?=?65.9?μg/mL; selectivity index (SI)?=?22.1) and amastigotes (IC50?=?37.9?μg/mL; SI?=?38.5) compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72?h of treatment (IC50?=?0.2?μg/mL). AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72?h. AEPa (100?μg/mL) promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72?h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells (50% cytotoxic concentration >1000?μg/mL).


Conclusion

AEPa presented antileishmanial activity against the promastigotes and amastigotes of L. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis.

Key words: Leishmanicidal activity, Leishmania infantum, Reactive oxygen species, Apoptosis, Physalis angulata

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