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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (7): 729-737.doi: 10.3736/jcim20080714

• Original Experimental Research • Previous Articles     Next Articles

Adrenal protein expressions after Pinggan Qianyang Formula treatment in hypertensive rats with liver-yang hyperactivity: a comparative proteomic analysis

Ying Zhang, Ze-qi Chen, Guang-wei Zhong()   

  1. Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China
  • Received:2007-10-29 Online:2008-07-20 Published:2008-07-15
  • Contact: ZHONG Guang-wei

Objective: To explore the pathogenic mechanism of liver-yang hyperactivity type of hypertension and to observe the effects of Pinggan Qianyang Formula (PGQYF), a compound of traditional Chinese herbals for calming the liver and suppressing yang, so as to provide experimental evidence for new marker proteins of drug therapy.
Methods: A rat model of liver-yang hyperactivity was prepared with spontaneous hypertensive rats (SHRs) by administration of Aconiti Praeparatae Decoction. Adrenal proteins were separated by 2D gel electrophoresis (2-DE). The differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database analysis.
Results: The rat model of liver-yang hyperactivity was successfully reproduced, and the PGQYF could decrease the grades of irritability, conjunctival congestion and systolic blood pressure of the rats (P<0.05, P<0.01). After analysis, twelve obviously differentially expressed proteins were found, eight of which were identified. The expression levels of isocitrate dehydrogenase and steroidogenic acute regulatory protein in the untreated group were up-regulated as compared with those in the normal control group, and down-regulated in the treatment group. The expression levels of ferritin light chain, elongation factor Tu, Rho GDP disassociation inhibitor 1, flavin reductase and basic transcription factor 3 in the untreated group were down-regulated as compared with those in the normal control group, and up-regulated in the treatment group.
Conclusion: Differentially expressed adrenal proteins in SHRs with live-yang hyperactivity are successfully identified. This approach may lay a foundation for the further investigation of pathogenic mechanisms in hypertension with liver-yang hyperactivity and the mechanisms of PGQYF treatment.

Key words: hypertension, liver-yang hyperactivity, Pinggan Qianyang Formula, proteomics, adrenal gland, rats

CLC Number: 

  • R966

Table 1

Changes of appearance and grades of irritability of the rats in different groups (Cases)"

Group n 3 weeks 6 weeks
Grade of irritability Conjunctival congestion Grade of irritability Conjunctival congestion
Normal control 10 1 0 0 0 1 0 0 0
Untreated 10 1 2 7 9 2 2 6 8
PGQYF 10 1 1 8 9 4 3 3 3

Table 2

Changes of volume of water the rats drank in different groups ($\bar{x}$±s, ml)"

Group n Volume of water
0 day 3 weeks 6 weeks
Normal control 10 32.42±2.16 35.20±2.46 33.62±3.52
Untreated 10 31.92±4.24 58.58±5.56**▲▲ 58.25±6.54**▲▲
PGQYF 10 33.35±3.47 56.90±5.72**▲▲ 35.60±4.33△△

Table 3

Dynamic state of blood pressures of the rats in different groups ($\bar{x}$±s, mmHg)"

Group n SBP
0 day 3 weeks 6 weeks
Normal control 10 106.52±6.34 105.54±5.85 106.58±6.45
Untreated 10 181.75±10.23** 198.59±9.89**▲▲ 202.00±7.25**▲▲
PGQYF 10 180.20±8.46** 198.23±8.97**▲▲ 175.41±6.58**△△

Figure 1

R-250 stained 2-DE image of adrenal proteins in rats (Coomassie brilliant blue-staining, pH 3-10, NL, 24 cm IPG gel) A: Normal control group; B: Untreated group; C: PGQYF group."

Figure 2

Partial 2-DE images of differential expression of rat adrenal proteins in three groups A: Normal control group; B: Untreated group; C: PGQYF group."

Figure 3

Mascot search results of differentially expressed adrenal proteins (Q923D2, Q99PT1)"

Table 4

Differentially expressed proteins after PGQYF treatment"

Access number Abbr. name Gene name Protein name Mr PI MALDI-TOF-MS Peptide
Matching Total Sequence coverage
Q8BFR5 Q497E7_MOUSE Tufm EF-Tu, mitochondrial 49 876 7.23 13 20 40
088844 AAD02919 Idh1 ICDH [NADP], cytoplasmic 47 030 6.48 15 59 41
Q99PT1 Q8BPIO_MOUSE Arhgdia Rho GDI1 22 991 5.20 9 16 56
P51557 A55455 StAR StAR, mitochondrial 31 891 8.56 13 35 40
Q923D2 BLVRB_MOUSE Blvrb FR 22 166 6.47 4 4 31
Q5U3Y8 Q5U3Y8_RAT Btf3 BTF3 17 688 6.85 9 24 50
P49945 FRIL2_MOUSE Ftl2 FLC2 20 756 6.37 5 7 50
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