Search JIM Advanced Search

Journal of Integrative Medicine ›› 2015, Vol. 13 ›› Issue (3): 194-200.doi: 10.1016/S2095-4964(15)60167-4

• Research Article • Previous Articles     Next Articles

Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats

Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning   

  1. School of Medical and Applied Sciences, CQ University Australia, Rockhampton, Queensland 4702, Australia
  • Received:2014-11-19 Accepted:2015-01-26 Online:2015-05-10 Published:2015-05-15

Crassostrea gigas oyster extract has been reported to have antioxidant, antihypertensive and lipid-lowering properties that may be useful for treating cardiovascular diseases. This study aimed to evaluate the effect of C. gigas oyster extract on cardiovascular function in tissues from healthy rats.
Single-cell microelectrode and isolated thoracic aortic organ bath studies were performed on tissues from 8-week-old healthy Wistar rats, using varying concentrations of C. gigas oyster extract. To elucidate a mechanism of action for the oyster's vasoactive properties, concentration response curves were carried out in the presence of a calcium channel inhibitior (verapamil), a nitric oxide synthase inhibitor (NG-nitro-L-arginine methyl ester), a potassium channel inhibitor (4-aminopyridine), in addition to the α-adrenoceptor inhibitor prazosin.
Oyster solution at 7 500 mg/mL inhibited noradrenaline-induced contraction in isolated aortic rings. Cardiac electrophysiology results showed that neither concentration of oyster solution was able to significantly reduce action potential duration at all phases of repolarisation in left ventricular papillary muscles from healthy animals.
When administered to healthy vascular tissue, C. gigas oyster extract inhibits contraction induced by noradrenaline. This effect is likely to be mediated through α-adrenoceptor inhibition, and to a lesser extent, calcium modulating activity.

Key words: Crassostrea, Hypertension, Calcium channels, Receptors, Adrenergic, Rats

[1] Yuan R, Lin Y . Traditional Chinese medicine: an approach to scientific proof and clinical validation. Pharmacol Ther, 2000,86(2):191-198
doi: 10.1016/S0163-7258(00)00039-5 pmid: 10799714
[2] Li WL, Zheng HC, Bukuru J, De Kimpe N . Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J Ethnopharmacol, 2004,92(1):1-21
doi: 10.1016/j.jep.2003.12.031
[3] Vuksan V, Sievenpiper JL . Herbal remedies in the management of diabetes: lessons learned from the study of ginseng. Nutr Metab Cardiovasc Dis, 2005,15(3):149-160
doi: 10.1016/j.numecd.2005.05.001 pmid: 15955462
[4] Vignesh S, Raja A, James RA . Marine drugs: implications and future studies. Int J Pharmacol, 2011,7(1):22-30
doi: 10.3923/ijp.2011.22.30
[5] Watanabe M, Fuda H, Jin S, Sakurai T, Hui SP, Takeda S, Watanabe T, Koike T, Chiba H . A phenolic antioxidant from the Pacific oyster(Crassostrea gigas) inhibits oxidation of cultured human hepatocytes mediated by diphenyl-1-pyrenylphosphine. Food Chem, 2012,134(4):2086-2089
doi: 10.1016/j.foodchem.2012.04.001
[6] Lund EK . Health benefits of seafood; is it just the fatty acids? Food Chem. 2013; 140(3):413-420.
doi: 10.1016/j.foodchem.2013.01.034 pmid: 23601384
[7] Shiozaki K, Shiozaki M, Masuda J, Yamauchi A, Ohwada S, Nakano T, Yamaguchi T, Saito T, Muramoto K, Sato M . Identification of oyster-derived hypotensive peptide acting as angiotensin-I-converting enzyme inhibitor. Fish Sci, 2010,76(5):865-872
doi: 10.1007/s12562-010-0264-0
[8] Jao CL, Huang SL, Hsu KC . Angiotensin I-converting enzyme inhibitory peptides: Inhibition mode, bioavailability, and antihypertensive effects. Biomedicine, 2012,2(4):130-136
doi: 10.1016/j.biomed.2012.06.005
[9] Wang Q, Li W, He Y, Ren D, Kow F, Song L, Yu X . Novel antioxidative peptides from the protein hydrolysate of oysters(Crassostrea talienwhanensis). Food Chem, 2014,145:991-996
doi: 10.1016/j.foodchem.2013.08.099
[10] Wu G . Amino acids: metabolism, functions, and nutrition. Amino Acids, 2009,37(1):1-17
[11] Palmi M, Youmbi GT, Fusi F, Sgaragli GP, Dixon HB, Frosini M, Tipton KF . Potentiation of mitochondrial Ca2+ sequestration by taurine. Biochem Pharmacol, 1999,58(7):1123-1131
doi: 10.1016/S0006-2952(99)00183-5 pmid: 10484070
[12] Li F, Abatan OI, Kim H, Burnett D, Larkin D, Obrosova IG, Stevens MJ . Taurine reverses neurological and neurovascular deficits in Zucker diabetic fatty rats. Neurobiol Dis, 2006,22(3):669-676
doi: 10.1016/j.nbd.2006.01.012 pmid: 16624563
[13] Abebe W . Effects of taurine on the reactivity of aortas from diabetic rats. Life Sci, 2008,82(5-6):279-289
doi: 10.1016/j.lfs.2007.11.012 pmid: 18174109
[14] Ito T, Kimura Y, Uozumi Y, Takai M, Muraoka S, Matsuda T, Ueki K, Yoshiyama M, Ikawa M, Okabe M, Schaffer SW, Fujio Y, Azuma J . Taurine depletion caused by knocking out the taurine transporter gene leads to cardiomyopathy with cardiac atrophy. J Mol Cell Cardiol, 2008,44(5):927-937
doi: 10.1016/j.yjmcc.2008.03.001
[15] Niu LG, Zhang MS, Liu Y, Xue WX, Liu DB, Zhang J, Liang YQ . Vasorelaxant effect of taurine is diminished by tetraethylammonium in rat isolated arteries. Eur J Pharmacol, 2008,580(1-2):169-174
doi: 10.1016/j.ejphar.2007.10.039 pmid: 17997400
[16] Rashid K, Das J, Sil PC . Taurine ameliorates alloxan induced oxidative stress and intrinsic apoptotic pathway in the hepatic tissue of diabetic rats. Food Chem Toxicol, 2013,51:317-329
doi: 10.1016/j.fct.2012.10.007
[17] Tapiero H, Tew KD . Increased glutathione expression in cells induced by Crassostera gigas extract(JCOE). Biomed Pharmacother, 1996,50(3-4):149-153
doi: 10.1016/0753-3322(96)85289-9 pmid: 8881371
[18] Yoshikawa TY, Naito Y, Masui K, Fujii T, Boku Y, Nakagawa S, Yoshida N, Kondo M . Free radical-scavenging activity of Crassostera gigas extract(JCOE). Biomed Pharmacother, 1997,51(8):328-332
doi: 10.1016/S0753-3322(97)88050-X pmid: 9436525
[19] Tanaka K, Nishizono S, Kugino K, Tamari M, Kurumiya M, Abe N, Ikeda I . Effects of dietary oyster extract on lipid metabolism, blood pressure, and blood glucose in SD rats, hypertensive rats, and diabetic rats. Biosci Biotechnol Biochem, 2006,70(2):462-470
doi: 10.1271/bbb.70.462
[20] Jabbar S, Khan MT, Choudhuri MS, Sil BK . Bioactivity studies of the individual ingredients of the Dashamularishta. Pak J Pharm Sci, 2004,17:9-17
pmid: 16414581
[21] Fenning A, Harrison G, Rose’meyer R, Hoey A , Brown L.l-Arginine attenuates cardiovascular impairment in DOCA-salt hypertensive rats. Am J Physiol Heart Circ Physiol, 2005,289(4):H1408-H1416
doi: 10.1152/ajpheart.00140.2005
[22] Wilson J, Hayes M, Carney B . Angiotensin-I-converting enzyme and prolyl endopeptidase inhibitory peptides from natural sources with a focus on marine processing by-products. Food Chem, 2011,129(2):235-244
doi: 10.1016/j.foodchem.2011.04.081
[23] Li Z, Zhang M, Liang Y . Endothelium-independent vasorelaxant effect of taurine on rat aorta rings. Zhongguo Zhong Yao Za Zhi, 2009,34(3):332-335
pmid: 19445161
[24] Xue W, Zhang M, Li J, Wu D, Niu L, Liang Y . Effects of taurine on aortic rings isolated from fructose-fed insulin resistance Sprague-Dawley rat are changed. Cardiovasc Drugs Ther, 2008,22(6):461-468
doi: 10.1007/s10557-008-6124-9
[25] Piascik MT, Soltis EE, Piascik MM, Macmillan LB . α-Adrenoceptors and vascular regulation: Molecular, pharmacologic and clinical correlates. Pharmacol Ther, 1996,72(3):215-241
doi: 10.1016/S0163-7258(96)00117-9 pmid: 9364576
[26] Docherty JR . Subtypes of functional α1-adrenoceptor. Cell Mol Life Sci, 2010,67(3):405-417
doi: 10.1007/s00018-009-0174-4
[27] Martínez-Nieves B, Dunbar JC . Vascular dilatatory responses to sodium nitroprusside(SNP) and α-adrenergic antagonism in female and male normal and diabetic rats. Exp Biol Med, 1999,222(1):90-98
doi: 10.1111/pse.1999.222.issue-1
[28] Moreira IS . Structural features of the G-protein/GPCR interactions. Biochim Biophys Acta, 2014,1840(1):16-33
doi: 10.1016/j.bbagen.2013.08.027 pmid: 24016604
[29] Joseph BK, Thakali KM, Moore CL, Rhee SW . Ion channel remodeling in vascular smooth muscle during hypertension: Implications for novel therapeutic approaches. Pharmacol Res, 2013,70(1):126-138
doi: 10.1016/j.phrs.2013.01.008
[30] Scholz EP, Zitron E, Katus HA, Karle CA . Cardiovascular ion channels as a molecular target of flavonoids. Cardiovasc Ther, 2010,28(4):e46-e52
doi: 10.1111/j.1755-5922.2010.00212.x pmid: 20633021
[31] Li P, Chen J, Wang J, Zhou W, Wang X, Li B, Tao W, Wang W, Wang Y, Yang L . Systems pharmacology strategies for drug discovery and combination with applications to cardiovascular diseases. J Ethnopharmacol, 2014,151(1):93-107
doi: 10.1016/j.jep.2013.07.001 pmid: 23850710
[32] Satoh H, Sperelakis N . Review of some actions of taurine on ion channels of cardiac muscle cells and others. Gen Pharmacol, 1998,30(4):451-463
[33] Wang Y, Cheng J, Tandan S, Jiang M , McCloskey DT, Hill JA. Transient-outward K+ channel inhibition facilitates L-type Ca2+ current in heart. J Cardiovasc Electrophysiol, 2006,17(3):298-304
doi: 10.1111/j.1540-8167.2006.00362.x pmid: 16643405
[34] Friedrich O, Wagner S, Battle AR, Schurmann S, Martinac B . Mechano-regulation of the beating heart at the cellular level - Mechanosensitive channels in normal and diseased heart. Prog Biophys Mol Biol, 2012,110(2-3):226-238
doi: 10.1016/j.pbiomolbio.2012.08.009
[35] Charpentier F, Mérot J, Loussouarn G, Baro I . Delayed rectifier K+ currents and cardiac repolarization. J Mol Cell Cardiol, 2010,48(1):37-44
doi: 10.1016/j.yjmcc.2009.08.005 pmid: 19683534
[36] Dobrzynski H, Anderson RH, Atkinson A, Borbas Z , D’Souza A, Fraser JF, Inada S, Logantha SJ, Monfredi O, Morris GM, Moorman AF, Nikolaidou T, Schneider H, Szuts V, Temple IP, Yanni J, Boyett MR. Structure, function and clinical relevance of the cardiac conduction system, including the atrioventricular ring and outflow tract tissues. Pharmacol Ther, 2013,139(2):260-288
doi: 10.1016/j.pharmthera.2013.04.010
[37] Grandi E, Pasqualini FS, Pes C, Corsi C, Zaza A, Severi S . Theoretical investigation of action potential duration dependence on extracellular Ca2+ in human cardiomyocytes. J Mol Cell Cardiol, 2009,46(3):332-342
doi: 10.1016/j.yjmcc.2008.12.002
[38] Curran J, Brown KH, Santiago DJ, Pogwizd S, Bers DM, Shannon TR . Spontaneous Ca waves in ventricular myocytes from failing hearts depend on Ca2+-calmodulin-dependent protein kinase II. J Mol Cell Cardiol, 2010,49(1):25-32
doi: 10.1016/j.yjmcc.2010.03.013
[39] Satoh H . Modulations by taurine of the spontaneous action potentials in right atrial muscles of rats. Gen Pharmacol, 1998,30(2):209-212
doi: 10.1016/S0306-3623(97)85720-5 pmid: 9502175
[1] Shuai Chen, Jin Hu, Deng-cheng Lu, Hong-yi Liu, Shan-shan Wei. Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder, a traditional Chinese medicine formula. Journal of Integrative Medicine, 2022, 20(1): 73-82.
[2] Rakhi Chakraborty, Swarnendu Roy. Angiotensin-converting enzyme inhibitors from plants: A review of their diversity, modes of action, prospects, and concerns in the management of diabetes-centric complications. Journal of Integrative Medicine, 2021, 19(6): 478-492.
[3] Pranay Soni, Rajesh Choudhary, Surendra H. Bodakhe. Effects of a novel isoflavonoid from the stem bark of Alstonia scholaris against fructose-induced experimental cataract. Journal of Integrative Medicine, 2019, 17(5): 374-382.
[4] Sonia Chew Wen Phang, Uma Devi Palanisamy, Khalid Abdul Kadir. Effects of geraniin (rambutan rind extract) on blood pressure and metabolic parameters in rats fed high-fat diet. Journal of Integrative Medicine, 2019, 17(2): 100-106.
[5] Ralph Fernando Rosas, Aline Armiliato Emer, Ana Paula Batisti, Daniela Dero Ludtke, Bruna Lenfers Turnes, Franciane Bobinski, Francisco José Cidral-Filho, Daniel Fernandes Martins. Far infrared-emitting ceramics decrease Freund’s adjuvant-induced inflammatory hyperalgesia in mice through cytokine modulation and activation of peripheral inhibitory neuroreceptors. Journal of Integrative Medicine, 2018, 16(6): 396-403.
[6] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[7] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[8] Nathalia Gomes Ribeiro de Moura, Ivan Cordovil, Arthurde Sá Ferreira. Traditional Chinese medicine wrist pulse-taking is associated with pulse waveform analysis and hemodynamics in hypertension. Journal of Integrative Medicine, 2016, 14(2): 100-113.
[9] Nouran A. Aleyeidi, Khaled S. Aseri, Shadia M. Matbouli, Albaraa A. Sulaiamani, Sumayyah A. Kobeisy. Effects of wet-cupping on blood pressure in hypertensive patients: A randomized controlled trial. Journal of Integrative Medicine, 2015, 13(6): 391-399.
[10] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[11] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[12] Ya Yuwen, Yu-qi Liu, Yan-ping Wang, Jin-gang Dai, Da-sheng Liu, Yue-xi Wang, Xue-jie Han. The add-on effect of a Chinese herbal formula for patients with resistant hypertension: Study protocol for a pilot cohort study. Journal of Integrative Medicine, 2015, 13(2): 122-128.
[13] Vamsi Reddy, Arvind Sridhar, Roberto F. Machado, Jiwang Chen. High sodium causes hypertension: Evidence from clinical trials and animal experiments. Journal of Integrative Medicine, 2015, 13(1): 1-8.
[14] Yong-hong Lian, Mei-xia Fang, Li-guo Chen. Constructing protein-protein interaction network of hypertension with blood stasis syndrome via digital gene expression sequencing and database mining. Journal of Integrative Medicine, 2014, 12(6): 476-482.
[15] Cheng Huang​. Natural modulators of liver X receptors. Journal of Integrative Medicine, 2014, 12(2): 76-85.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228