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Journal of Integrative Medicine ›› 2022, Vol. 20 ›› Issue (1): 73-82.doi: 10.1016/j.joim.2021.11.007

• Original Experimental Research • Previous Articles     Next Articles

Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder, a traditional Chinese medicine formula

Shuai Chena, Jin Hua, Deng-cheng Lua, Hong-yi Liub, Shan-shan Weia,c   

  1. a. College of Basic Medical Sciences, Yunnan University of Chinese Medicine, Kunming 650500, Yunnan Province, China
    b. Department of Endocrinology, Yunnan Traditional Chinese Medicine Hospital, Kunming 650021, Yunnan Province, China
    c. Key Laboratory of Syndrome Micro-Differentiation of Yunnan Province, Kunming 650500, Yunnan Province, China
  • Received:2020-12-07 Accepted:2021-08-05 Online:2022-01-12 Published:2021-12-22
  • Contact: Shan-shan Wei E-mail:sswei@vip.163.com

Objective

Numerous studies have demonstrated the close relationship between chronic stress and blood pressure (BP). Hypertensive subjects exhibit exaggerated reactions to stress, especially higher BP. The mechanisms by which stress affects pre-existing hypertension still need to be explored. Danzhi Xiaoyao Powder (DP), a historical traditional Chinese medicine formula, is a promising treatment for BP control in hypertensive patients under stress. The present study investigated the metabolomic disruption caused by chronic stress and the treatment effect and mechanism of DP.


Methods

Spontaneously hypertensive rats (SHRs) were subjected to chronic restraint stress (CRS) for 4 weeks. BP was measured via the tail-cuff method, and anxiety-like behavior was quantified using the elevated-plus-maze test. Meanwhile, DP was administered intragastrically, and its effects were observed. Global metabolomic analysis was performed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, followed by multivariate statistical analysis to detect differential metabolites and pathways.


Results

DP alleviated the CRS-induced increase in BP and anxiety-like behavior. Systematic metabolic di?erences were found among the three study groups. A total of 29 differential plasma metabolites were identified in both positive- and negative-ion modes. These metabolites were involved in triglyceride metabolism, amino acid (phenylalanine, tryptophan, and glycine) metabolism, and steroid hormone pathways.


Conclusion

These findings expose the metabolomic disturbances induced by chronic stress in SHRs and suggest an innovative treatment for this disorder.

Key words: Hypertension, Stress, Metabolomics, Danzhi Xiaoyao Powder

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