金雀异黄素对Ⅱ型胶原诱导型关节炎大鼠成纤维样滑膜细胞分泌白细胞介素1β和肿瘤坏死因子α的影响

doi:10.3736/jcim20090707

Abstract

Objective

To investigate the effects of genistein (Gen) on interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) secreted by fibroblast-like synoviocytes (FLSs) of rats with type Ⅱ collagen-induced arthritis (CIA).

Methods

Type Ⅱ collagen was injected to induce arthritis in rats, and arthritis index was applied to evaluate whether the arthritis was induced successfully. The primary FLSs were separated from synovial membranes by using type Ⅱ collagenase digestion. Then the expression of vascular cell adhesion molecule-1 (VCAM-1) was estimated by flow cytometry (FCM). After addition of different concentrations of Gen into the FLSs, IL-1β and TNF-α contents in the supernatants were measured by enzyme-linked immunosorbent assay.
Results

Three days after collagen injection, the rats started to develop arthrocele and the arthritis index increased gradually. The arthritis index of CIA group was significantly higher than that of the control group. The expression of VCAM-1 was up to 85.5% in the 4th generation FLSs, indicating that most part of the cultured cells were type-B synoviocytes. After administration of different concentrations of Gen (100, 200, 400 μmol/L), the contents of IL-1β and TNF-α in supernatants of FLSs were decreased dose-dependently.
Conclusion

Genistein can suppress the secretion of TNF-α and IL-1β in FLSs dose-dependently, which may be one of the mechanisms for genistein in inhibiting the arthromeningitis of CIA rats.

Key words: Arthritis, Genistein, Synoviocyte, Interleukin-ip, Tumor necrosis factor-a, Rats

Bo Gao, Yu Zhang, Li Ma, Xue-zeng Zhang. Effects of genistein on interleukin-1β and tumor necrosis factor-α secreted by fibroblast-like synoviocytes isolated from type Ⅱ collagen-induced arthritis rats. Journal of Chinese Integrative Medicine, 2009, 7(7): 636-641.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090707

http://www.jcimjournal.com/EN/Y2009/V7/I7/636

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References 17

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Group	n	Score of arthritis index
Group	n	1st week	2nd week	3rd week	4th week	5th week	6th week
Control	5	0	0	0	0	0	0
CIA	5	5.20±1.48*	7.40±2.30*	8.40±2.40*	7.80±1.30*	7.60±1.67*	7.40±1.34*

Group	n	Drug concentration	TNF-α	IL-1β
Control	6		1.005±0.017^▲	1.001±0.038^▲
CIA	6		1.780±0.053*^▲	1.405±0.054*
MTX	6	1 mg/L	1.505±0.032^△	1.231±0.044^△
Gen1	6	100 μmol/L	1.742±0.039^▲	1.366±0.055^▲
Gen2	6	200 μmol/L	1.484±0.041^△□	1.250±0.071^△
Gen3	6	400 μmol/L	1.383±0.028^△▲□	1.140±0.048^△▲□

Effects of genistein on interleukin-1β and tumor necrosis factor-α secreted by fibroblast-like synoviocytes isolated from type Ⅱ collagen-induced arthritis rats

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