去甲斑蝥素两种注射剂型小鼠急性毒性比较

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Comparison of acute toxicity of two preparations of norcantharidin in mice

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doi:10.3736/jcim20070115

Objective: To compare the acute toxicity of common injection and sustained-release preparation of norcantharidin for mice so as to identify the attenuation of the sustained-release preparation of norcantharidin.

Methods: The poloxamer 407 was used as a sustained-release vehicle for topical administration of norcantharidin, and the acute toxicity of mice treated with common injection and sustained-release preparation of norcantharidin was observed. The median lethal dose (LD₅₀) was calculated by Bliss software.

Results: The symptoms of mice were similar between the two groups, but the appearance of symptoms in norcantharidin/poloxamer 407 group was 4 hours later than that in norcantharidin group. The LD₅₀ of norcantharidin administered through vein injection was 12.6 mg/kg. The LD₅₀ of norcantharidin/poloxamer 407 administered through intraperitoneal injection, intrahepatic injection and intramuscular injection were 19.9, 19.1 and 20.9 mg/kg, respectively, and the LD₅₀ of the common preparation were 13.0, 13.1 and 15.1 mg/kg, respectively.

Conclusion: The norcantharidin/poloxamer 407 is less toxic than the equivalent dose of norcantharidin, mainly because norcantharidin/poloxamer 407 may release norcantharidin sustainedly, thus reducing norcantharidin concentration in blood.

Key words: sustained-release preparations, poloxamer 407, acute toxicity tests

CLC Number:

R965.3

Bai Li, Li-feng Zhu, Ya-ni Zhang, Wei Gu. Comparison of acute toxicity of two preparations of norcantharidin in mice. Journal of Chinese Integrative Medicine, 2007, 5(1): 74-77.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070115

http://www.jcimjournal.com/EN/Y2007/V5/I1/74

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