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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (1): 74-77.doi: 10.3736/jcim20070115

• Original Experimental Research • Previous Articles     Next Articles

Comparison of acute toxicity of two preparations of norcantharidin in mice

Bai Li, Li-feng Zhu, Ya-ni Zhang, Wei Gu   

  1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Online:2007-01-31 Published:2007-01-21

Objective: To compare the acute toxicity of common injection and sustained-release preparation of norcantharidin for mice so as to identify the attenuation of the sustained-release preparation of norcantharidin. 

Methods: The poloxamer 407 was used as a sustained-release vehicle for topical administration of norcantharidin, and the acute toxicity of mice treated with common injection and sustained-release preparation of norcantharidin was observed. The median lethal dose (LD50) was calculated by Bliss software. 

Results: The symptoms of mice were similar between the two groups, but the appearance of symptoms in norcantharidin/poloxamer 407 group was 4 hours later than that in norcantharidin group. The LD50 of norcantharidin administered through vein injection was 12.6 mg/kg. The LD50 of norcantharidin/poloxamer 407 administered through intraperitoneal injection, intrahepatic injection and intramuscular injection were 19.9, 19.1 and 20.9 mg/kg, respectively, and the LD50 of the common preparation were 13.0, 13.1 and 15.1 mg/kg, respectively. 

Conclusion: The norcantharidin/poloxamer 407 is less toxic than the equivalent dose of norcantharidin, mainly because norcantharidin/poloxamer 407 may release norcantharidin sustainedly, thus reducing norcantharidin concentration in blood.

Key words: sustained-release preparations, poloxamer 407, acute toxicity tests

CLC Number: 

  • R965.3

Table 1

Injected volume and dose of two preparations of norcantharidin in mice"

Table 1

Acute toxicity of two preparations of norcantharidin in mice"

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