Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (8): 781-784.doi: 10.3736/jcim20100809

Special Issue: Traditional Chinese Medicine

• Original Experimental Research • Previous Articles     Next Articles

Comparative study on hypoglycemic effects of different traditional Chinese medicine treatments in rats with diabetes mellitus induced by alloxan

 JingWanga, QingWangb, Zhen-zhenWangb, ZhuoFengb, Shao-yunLiub, Qi-qiZhangb, Qi-wenCaib,Jing-juan Panb   

  1. a Experimental Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b School of Basic Medical Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2010-05-10 Accepted:2010-06-03 Online:2010-08-20 Published:2010-08-15

Objective

To observe hypoglycemic effects of Yunu Decoction, Zuogui Pill and Shenqi Pill, three compound traditional Chinese herbal medicines, in treatment of diabetes mellitus induced by alloxan in rats, and to compare the therapeutic effects among the three recipes for nourishing yin, clearing away heat, and nourishing yin and warming yang.
Methods

Diabetes mellitus was induced in rats with alloxan at a dose of 60 mg/kg via tail vain injection. The diabetic rats were randomly divided into four groups: alloxan model group, Yunu Decoction-treated group, Zuogui Pill-treated group and Shenqi Pill-treated group. Rats in the three recipe groups were administered intragastrically with water extraction of Yunu Decoction, Zuogui Pill, and Shenqi Pill accordingly for 10 days. Then the level of blood glucose was measured by glucose oxidase method and the glucose tolerance was determined.
Results

Compared with the normal rats, blood glucose level in the alloxan model group was obviously increased (P<0.05). Glucose levels in the three recipe groups were obviously decreased as compared with the alloxan model group (P<0.05), and glucose level in the Yunu Decoction-treated group after treatment was significant lower than before treatment (P<0.05). The glucose tolerance test indicated that rats in the alloxan model and three recipe groups revealed impaired glucose tolerance as compared with the normal rats, and there were no significant differences between the alloxan model group and the three recipe groups.
Conclusion

Yunu Decoction, Zuogui Pill and Shenqi Pill can effectively decrease the glucose level of the rats with diabetes mellitus induced by alloxan, and Yunu Decoction showed the best therapeutic effects. The glucose tolerance test shows that the three recipes cannot correct the abnormal metabolism of glucose.

Key words: Diabetes mellitus, Alloxan, Nourishing yin, Clearing away heat, Warming yang

Table 1

Effects of Yunu Decoction, Zuogui Pill, Shenqi Pill on glucose level of diabetic rats"

Figure 1

Trends of glucose levels after exogenous glucose stimulation in different groups"

[1] Ye J, Han LS, Qiu WJ, Shen YN, Gu XF . Screening of type 2 diabetes mellitus and those with high risk among children and adolescents with obesity[J]. Zhongguo Nei Fen Mi Dai Xie Za Zhi, 2004,20(2):132-135
doi: 10.3760/j.issn:1000-6699.2004.02.017
叶军, 韩连书, 邱文娟, 沈永年, 顾学范 . 儿童、青少年肥胖者2型糖尿病和高危者筛查[J]. 中国内分泌代谢杂志, 2004,20(2):132-135
doi: 10.3760/j.issn:1000-6699.2004.02.017
[2] Huang M, Wang SK, Wang XF, Dai DZ, Wang ZZ . Influences on serum insulin and glucose by different fasting time in alloxan diabetic mice[J]. Zhongguo Yao Ke Da Xue Xue Bao, 2001,32(3):217-220
doi: 10.3321/j.issn:1000-5048.2001.03.015
黄敏, 王书奎, 王小峰, 戴德哉, 王自正 . 不同禁食时间对四氧嘧啶糖尿病小鼠模型的血清胰岛素和血糖的影响[J]. 中国药科大学学报, 2001,32(3):217-220
doi: 10.3321/j.issn:1000-5048.2001.03.015
[3] Wen XH, Shen CY, Lei J, Yang ZW, Wang YP . Dynamic observation on the intraperitioneal glucose tolerance test in the non-hyperglycemic rats treated with streptozotocin[J]. Chongqing Yi Ke Da Xue Xue Bao, 2008,33(12):1467-1470
文晓红, 沈成义, 雷军, 杨正伟, 王亚平 . 链脲佐菌素未致高血糖大鼠腹腔葡萄糖耐量试验的动态观察[J]. 重庆医科大学学报, 2008,33(12):1467-1470
[4] Ma YC. Experimental traditional Chinese medicine[M]. Beijing: United Publishing House of Beijing Medical University and Chinese Union Medical University,2000:
马超英 . 实验中医学[M]. 北京: 北京医科大学中国协和医科大学联合出版社,2000:
[5] Zhu YF, Chen SR . Mechanism of pancreatic islet β cell injury in type 1 diabetes[J]. Shantou Da Xue Yi Xue Yuan Xue Bao, 2002,15(3):173-175
朱远锋, 陈慎仁 . 1型糖尿病胰岛β-细胞损伤机制[J]. 汕头大学医学院学报, 2002,15(3):173-175
[6] Ye HH, Yuan JF, Li M, Wang YJ, Dong G, Yang LP, Zhang GZ, Zhang XF, Zeng L . Different effects of alloxan on diabetic model duplication in male and female animals[J]. Zhongguo Bi Jiao Yi Xue Za Zhi, 2010,20(1):19-22
doi: 10.3969/j.issn.1671-7856.2010.01.005
叶华虎, 袁菊芳, 李敏, 王艳静, 董罡, 杨丽萍, 张广州, 张小飞, 曾林 . 四氧嘧啶诱发糖尿病模型效果的性别差异[J]. 中国比较医学杂志, 2010,20(1):19-22
doi: 10.3969/j.issn.1671-7856.2010.01.005
[7] Tong XL, Zhao LH, Chen L, Wang J . Development of study on prevention and treatment of diabetes by traditional Chinese medicine[J]. Yao Pin Ping Jia, 2009,6(12):467-470
doi: 10.3969/j.issn.1672-2809.2009.12.002
仝小林, 赵林华, 陈良, 王佳 . 糖尿病中医药防治研究进展[J]. 药品评价, 2009,6(12):467-470
doi: 10.3969/j.issn.1672-2809.2009.12.002
[8] Meng JB, Kang SQ, Kang SX, Yang Y, Zhou CG . Experimental study of diabetic models in rabbits induced by alloxan[J]. Hebei Yi Yao, 2009,31(1):19-20
doi: 10.3969/j.issn.1002-7386.2009.01.007
孟建波, 康胜群, 康素娴, 杨彦, 周晨光 . 四氧嘧啶诱导兔1型糖尿病模型的实验研究[J]. 河北医药, 2009,31(1):19-20
doi: 10.3969/j.issn.1002-7386.2009.01.007
[1] Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[2] Olakunle Bamikole Afolabi, Omotade Ibidun Oloyede, Shadrack Oludare Agunbiade. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. Journal of Integrative Medicine, 2018, 16(3): 192-198.
[3] Chun-song Hu, Tengiz Tkebuchava. SEEDi1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[4] Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[5] Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[6] Yee Chi Peggy Wong. Need of integrated dietary therapy for persons with diabetes mellitus and “unhealthy” body constitution presentations. Journal of Integrative Medicine, 2016, 14(4): 255-268.
[7] Ausanee Wanchai, Duangjai Phrompayak. Use of complementary and alternative medicine among Thai patients with type 2 diabetes mellitus. Journal of Integrative Medicine, 2016, 14(4): 297-305.
[8] Sulaiman Al-Eidi, SalwaTayel, Fatima Al-Slail, Naseem Akhtar Qureshi, Imen Sohaibani, Mohamed Khalil, Abdullah Mohammad Al-Bedah. Knowledge, attitude and practice of patients with type 2 diabetes mellitus towards complementary and alternative medicine. Journal of Integrative Medicine, 2016, 14(3): 187-196.
[9] Mohammad Hashem Hashempur, Mojtaba Heydari, Seyed Hamdollah Mosavat, Seyyed Taghi Heydari, Mesbah Shams. Complementary and alternative medicine use in Iranian patients with diabetes mellitus. Journal of Integrative Medicine, 2015, 13(5): 319-325.
[10] Ahmad Ghorbani. Clinical and experimental studies on polyherbal formulations for diabetes: Current status and future prospective. Journal of Integrative Medicine, 2014, 12(4): 336-345.
[11] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[12] Rajadurai Akilen​, Zeller Pimlott​, Amalia Tsiami​, Nicola Robinson​. The use of complementary and alternative medicine by individuals with features of metabolic syndrome. Journal of Integrative Medicine, 2014, 12(3): 171-174.
[13] Hai-li Jiang, Jing-jing Ni, Wei-fei Zhang, Wen-jin Huang​, Ming-yue Zhou​, Wen-jun Sha​, Jun-yan Li​, Fu-feng Li​, Zhu Ting​​, Xin Xia, Jun Zhang​, Yuan-dong Shen​, Li-gang Zhou​. The role of central nervous system on hypoglycemia and the feasibility of the brain theory in traditional Chinese medicine on treatment of diabetes mellitus. Journal of Integrative Medicine, 2014, 12(1): 1-6.
[14] Dilpesh Jain​, Manish Kumar Bansal, Rahul Dalvi​, Aman Upganlawar​, Rahul Somani​. Protective effect of diosmin against diabetic neuropathy in experimental rats. Journal of Integrative Medicine, 2014, 12(1): 35-41.
[15] Abdullah Bamosa. Nigella sativa is a safe herbal product. Journal of Integrative Medicine, 2014, 12(1): 66-66.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[2] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[3] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[4] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[5] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[6] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[7] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[8] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[9] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[10] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047