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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (1): 86-89.doi: 10.3736/jcim20100116

• Original Experimental Research • Previous Articles     Next Articles

Comparative study of different therapeutic methods on autoimmune premature ovarian failure in mice

 Li Dong,Yuan Tan, Yu-jia Ping, Nan-sun Zhu   

  1. Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2009-09-09 Accepted:2009-11-10 Online:2010-01-20 Published:2010-01-15
  • Contact: Li Dong E-mail:dongli888@yahoo.com

Objective

To investigate the effects of different methods of treatment, including tonifying the kidney, activating blood and soothing the liver on mice with autoimmune premature ovarian failure (POF).
Methods

After preparing ovarian antigen with the ovarian tissue of female BALB/C mice, POF in female BALB/C mice was induced by immunization injection of the ovarian antigen on multiple subcutaneous sites and two posterior soles. The mice were treated with Liuwei Dihuang Pill, Shaofu Zhuyu Granule, Chaihu Shugan Pill respectively after the third immunization. Levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), prolactin (PRL), testosterone (T), anti-ovarian antibodies (AOAb) and anti-zona pellucida antibody (aZP) in sera were analyze by enzymelinked immunosorbent assay.
Results

Compared with the normal group, the levels of serum FSH and LH were increased and the level of E2was decreased in the untreated group. AOAb and aZP positive rates in the untreated group were obviously higher than those in the normal group. Liuwei Dihuang Pill could significantly reduce the levels of FSH and LH and increase the level of E2as compared with the untreated group, but there were no significant differences in the AOAb and aZP positive rates. Shaofu Zhuyu Granule and Chaihu Shugan Pill could significantly reduce the positive rates of AOAb and aZP as compared with the untreated group, but no significant effect on the levels of FSH, LH and E2were observed. The drugs had no effects on contents of PRL and T in sera.
Conclusion

Tonifying the kidney method can recover the ovarian function in POF mice mainly by regulating the hormone levels. Activating blood and soothing the liver methods can improve the humoral immune function in POF mice.

Key words: Premature ovarian failure, Therapy, Tonify the kidney, Activating blood, Soothe the liver, Mice

Table 1

Contents of serum FSH, LH, E2, PRL and T in mice in different groups ($\bar{x}±s$)"

Group n FSH (IU/L) LH (IU/L) E2 (ng/L) PRL (μg/L) T (nmol/L)
Normal 20 1.66±0.30 1.06±0.23 39.55±2.83 21.47±3.31 1.41±0.35
Untreated 20 2.69±0.43* 1.90±0.38* 17.01±2.39* 24.19±2.19 1.34±0.33
LWDHP 20 1.87±0.47 0.96±0.24 24.93±5.01* 24.11±3.76 1.46±0.25
SFZYG 20 2.21±0.44 1.47±0.25 19.00±1.89 25.02±2.88 1.34±0.12
CHSGP 20 2.58±0.56 1.75±0.18 16.61±2.76 23.67±4.33 1.36±0.25

Table 2

Incidences of serum positive AOAb and aZP in mice in different groups"

Group n AOAb-positive rate aZP-positive rate
Normal 20 0 0
Untreated 19 84.2% (16/19)* 78.9% (15/19)*
LWDHP 17 82.3% (14/17) 76.5% (13/17)
SFZYG 20 45.0% (9/20) 35.0% (7/20)
CHSGP 20 50.0% (10/20) 40.0% (8/20)
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