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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (4): 437-441.doi: 10.3736/jcim20070415

• Original Experimental Research • Previous Articles     Next Articles

Effects of warming spleen and stomach for dispersing phlegm recipe on expression of interleukin-8 in S180 tumor tissue in mice

Hai-zhen Dang, Pin-kang Wei, Xiao-feng Zhang, Zhan-wen Liu   

  1. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Online:2007-07-31 Published:2007-05-15

Objective: To observe the effects of warming spleen and stomach for dispersing phlegm recipe (Wenzhong Xiaotan Recipe, WZXTR), a compound traditional Chinese herbal medicine, on expression of interleukin-8 (IL-8) in S180 tumor tissue in mice, and to explore the effectiveness and its mechanism.Methods: Fifty mice were randomly divided into five groups: untreated group, cold water-treated group, cold water and WZXTR-treated group, cold water and dispersing phlegm and eliminating stagnation recipe (Xiaotan Sanjie Recipe, XTSJR, another compound traditional Chinese herbal medicine) treated group, cold water and tegafur-treated group. Mice in the latter 4 groups were fed 0-4 ℃ cold distilled water 10 ml/(kg·d) one week before tumor inoculation for 3 weeks. Mice in the 5 groups were inoculated neoplastic cells which were diluted 2×107/ml with normal saline in the right armpit at the second week. Forty-eight hours later, the mice were given drugs intragastrically (WZXTR, XTSJR and tegafur, respectively). The mice were continually intervened by cold water in the morning and given drugs in the afternoon. Mice in the cold water-treated group were given normal saline, and nothing was given in untreated group. Morphology appearance and changes of rectal temperature were observed, and the tumor weight and volume were measured and inhibitory rate was calculated 22 days after tumor inoculation. IL-8 was detected by avidin-biotin system-enzyme-labeled immunosorbent assay (ABC-ELISA) and real-time reverse polymerase chain reaction (real-time PCR).Result: The content of IL-8 in tumor tissue of the untreated group was significantly higher than that of the other groups (P<0.01), and that of the cold water-treated group was higher than that of the cold water and WZXTR-treated group (P<0.01) and the cold water and XTSJR-treated group (P<0.05), respectively. The gray scale value of cDNA amplification strap was descent more evidently in both the cold water and WZXTR-treated group and the cold water and XTSJR-treated group as compared with that of the cold water-treated group and the untreated group (P<0.05).Conclusion: WZXTR can restrain tumor growth, and the mechanism may be that it can degrade the expression of IL-8 in S180 tumor.

Key words: tumor, interleukin-8, mice, traditional Chinese medicine

CLC Number: 

  • 1977(2007)04-0437

Table 1

Tumor weight, volume and inhibitory rate of transplanted tumor"

Groupn Tumor weight (x±s, g)Tumor volume (x±s, cm3)Inhibitory rate (%)
Cold water-treated102.63±0.283.38±0.31
Cold water and XTSJR-treated101.02±0.02**△△1.08±0.02**△△61.18
Cold water and WZXTR-treated100.97±0.03**△△1.12±0.04**△△63.23
Cold water and tegafur-treated101.19±0.03**△△1.12±0.02**△△54.71

Table 2

Changes of IL-8 in tumor tissue of transplanted tumor (ヌ±S, pg/mg)"

Groupn IL-8
Cold water-treated1019.24±8.12**▲▲
Cold water and XTSJR-treated1011.37±6.61**△
Cold water and WZXTR-treated109.35±5.51**
Cold water and tegafur-treated1018.63±3.81**▲▲

Figure 1

Expression of IL-8 mRNA in S180 tumor tissue1: Cold water and tegafur-treated group; 2: Untreated group; 3: Cold water and WZXTR-treated group; 4: Cold water and XTSJR-treated group; 5: Cold water-treated group."

Table 3

Positive expression of gene fragment of IL-8(ヌ±S)"

Groupn Positive expression of
gene fragment of IL-8
Cold water-treated100.622±0.060
Cold water and XTSJR-treated100.354±0.042*△
Cold water and WZXTR-treated100.242±0.024*△
Cold water and tegafur-treated100.532±0.077*△
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