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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (1): 100-104.doi: 10.3736/jcim20110116

• Original Experimental Research • Previous Articles     Next Articles

Analgesic effect of extracts of Alpinia galanga rhizome in mice

Sahana Devadasa Acharya1, Sheetal Dinkar Ullal1(), Shivaraj Padiyar1, Yalla Durga Rao1, Kousthubha Upadhyaya2, Durga Pillai1, Vishnu Raj1   

  1. 1. Department of Pharmacology, Kasturba Medical College, Manipal University, Mangalore 575001, Karnataka, India
    2. Department of Dravyaguna, National Institute of Ayurveda, Jaipur 302002, Rajasthan, India
  • Received:2010-09-09 Accepted:2010-09-28 Online:2011-01-20 Published:2011-01-15

Objective: To evaluate the analgesic effect of extracts of Alpinia galanga (AG) rhizome in mice and elucidate the possible mechanism for its analgesic action.
Methods: Analgesic action of extracts of AG rhizome was studied in three experimental models of nociception. Albino mice of both sexes weighing 25 to 30 g were used in this study. For the hot-plate test, mice in the five groups with six in each received three different doses of ethanolic extracts of dried rhizome of AG suspended in 2% gum acacia orally, morphine subcutaneously and 2% gum acacia orally, respectively. Reaction time was observed after administration of vehicle or drugs. For the hot-plate test after naloxone pretreatment, mice in the five groups received naloxone subcutaneously 30 min prior to the administration of vehicle or drugs and reaction time was observed as explained above. In the writhing test, writhes were induced by injecting acetic acid intraperitoneally in another 30 mice which were randomly allocated to five groups of six in each and received three different doses of ethanolic extracts of dried rhizome of AG suspended in 2% gum acacia, aspirin suspended in 2% gum acacia and 2% gum acacia orally, respectively. The mice were observed individually for a period of 15 min and the number of writhes was recorded for each animal.
Results: AG treatment significantly increased the latency period in the hot-plate test at all three doses at 30, 60, 90 and 120 min time intervals compared with control group (P<0.05 or P<0.01). Naloxone pretreatment significantly reduced the latency period in hot-plate test for both AG and morphine groups as compared with corresponding groups that did not receive naloxone pretreatment (P<0.05 or P<0.01). AG at all doses significantly reduced the number of writhes compared with control group (P<0.01).
Conclusion: The study confirmed the analgesic effect of AG rhizome and hence justified its use in ethnomedicine for the treatment of pain due to various causes. The probable mechanism of its analgesic action may be central as well as peripheral.

Key words: Alpinia galanga, plant extracts, analgesics, pain measurement, receptors, opioid, mice

[1] Azaizeh H, Saad B, Cooper E, Said O . Traditional Arabic and Islamic medicine, a re-emerging health aid[J]. Evid Based Complement Alternat Med, 2010,7(4):419-424
doi: 10.1093/ecam/nen039
[2] Premanathan M, Rajendran S, Ramanathan T, Kathiresan K, Nakashima H, Yamamoto N . A survey of some Indian medicinal plants for anti-human immunodeficiency virus(HIV) activity[J]. Indian J Med Res, 2000,112:73-77
[3] Nadkarni KM. Dr. K. M. Nadkarni’s Indian materia medica(Volume 1)[M]. Mumbai: Popular Prakashan Pvt. Ltd, 1995: 77-78
[4] Tachakittirungrod S, Chowwanapoonpohn S . Comparison of antioxidant and antimicrobial activities of essential oils from Hyptis suaveolens and Alpinia galanga growing in northern Thailand[J]. J Nat Sci, 2007,6(1):31-42
[5] Lima JA , Oliveira AS, de Miranda AL, Rezende CM, Pinto AC.Anti-inflammatory and antinociceptive activities of an acid fraction of the seeds of Carpotroche brasiliensis(Raddi)(Flacourtiaceae)[J]. Braz J Med Biol Res, 2005,38(7):1095-1103
doi: 10.1590/S0100-879X2005000700013
[6] Vasconcelos SM , Reboucas Oliveira G, Mohana de Carvalho M, Rodrigues AC, Rocha Silveira E, Maria Franca Fonteles M, Florenco Sousa FC, Barros Viana GS.Antinociceptive activities of the hydroalcoholic extracts from Erythrina velutina and Erythrina mulungu in mice[J]. Biol Pharm Bull, 2003,26(7):946-949
doi: 10.1248/bpb.26.946
[7] Parmar NS, Prakash S. Evaluation of analgesic, anti-inflammatory and antipyretic activity. In: Parmar NS, Prakash S. Screening methods in pharmacology[M]. New Delhi: Narosa Publishing House, 2006: 211-338
[8] Almeida ER, Almeida RN, Navarro DS, Bhattacharryya J, Silva BA, Birnbaum JS . Central antinociceptive effect of a hydroalcoholic extract of Dioclea grandiflora seeds in rodents[J]. J Ethnopharmacol, 2003,88(1):1-4
doi: 10.1016/S0378-8741(03)00180-6
[9] Santos FA, Rao VS . Antiinflammatory and antinociceptive effects of 1,8-cineole a terpenoid oxide present in many plant essential oils[J]. Phytother Res, 2000,14(4):240-244
doi: 10.1002/(ISSN)1099-1573
[10] García MD , FernÍndez MA, Alvarez A, Saenz MT.Antinociceptive and anti-inflammatory effect of the aqueous extract from leaves of Pimenta racemosa var. ozua(Mirtaceae)[J]. J Ethnopharmacol, 2004,91(1):69-73
doi: 10.1016/j.jep.2003.11.018
[11] Nguelefack TB, Nana P, Atsamo AD, Dimo T, Watcho P, Dongmo AB, Tapondjou LA, Njamen D, Wansi SL, Kamanyi A . Analgesic and anticonvulsant effects of extracts from the leaves of Kalanchoe crenata(Andrews) Haworth(Crassulaceae)[J]. J Ethnopharmacol, 2006,106(1):70-75
doi: 10.1016/j.jep.2005.12.003
[12] Vinegar R, Schreiber W, Hugo R . Biphasic development of carrageenin edema in rats[J]. J Pharmacol Exp Ther, 1969,166(1):96-103
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