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Journal of Chinese Integrative Medicine ›› 2013, Vol. 11 ›› Issue (2): 125-134.

• Research Article • Previous Articles     Next Articles

Antidiarrheal properties of different extracts of Chinese herbal medicine formula Bao-Xie-Ning

Jing Lia, Xin-lin Wub, Yuling Chenc,d, Zhi Tanga, Yue-hong Xua, Jian-min Jianga, Yue-yu Gub, Jian-ping Chene, De-po Yanga, Dong-mei Wanga()   

  1. a. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, Guangdong Province, China
    b. Department of Traditional Chinese Medicine, the First Affiliated Hospital, Sun Yat-sen University,Guangzhou 510080, Guangdong Province, China
    c. Faculty of Pharmacy, University of Sydney, NSW 2006, Australia
    d. Traditional Medicine Chinic, Kiang Wu Hospital, Macao, China
    e. School of Chinese Medicine, University of Hong Kong, Hong Kong, China
  • Received:2012-10-08 Accepted:2012-12-14 Online:2013-03-10 Published:2013-03-15

Objective

Bao-Xie-Ning (BXN), a traditional Chinese herbal medicine (CHM) formula composed of Fructus Evodiae, Flos Caryophylli and Cortex Cinnamomi, and used for the treatment of infant diarrheal illness, was subject to systematic assessment for its putative multiple pharmacodynamic effects and pharmacological antidiarrheal mechanisms.


Methods

High-performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometric/mass spectrometry was developed and validated for identification and quantification of the main constituents in different extracts of BXN. Male Kunming mice weighing 20 to 25 g were used for detecting the antidiarrheal activity of the extracts. Ethanolic extract (EE), volatile oil extract (VOE), and aqueous extract (AE) of BXN were respectively subjected to pharmacodynamic and pharmacological comparison in assessing antidiarrheal effects with senna-induced diarrhea, castor oil-induced diarrhea, acetic acid-induced writhing assay, and isolated duodenum test.


Results

The highest yields of three detected components of BXN, rutaecarpine, eugenol and cinnamaldehyde were observed in EE. EE showed the most remarkable antidiarrheal activity in dose-dependent and time-dependent manners in both senna- and castor oil-induced diarrhea models, and presented dose-dependent analgesic activity in acetic acid-induced algesthesia model. In addition, EE extract of BXN also exhibited strong antimobility action on the intestine and strongest depression on spontaneous contraction of isolated duodenum.


Conclusion

Ethanol extraction is an efficient method to extract the active constituents of BXN. BXN extract demonstrated multiple pharmacological activities affecting the main mechanisms of diarrhea, which validated BXN’s usage in the comprehensive clinical treatment of diarrhea.

Key words: Drugs, Chinese herbal, Antidiarrhea, Pain, Analgesic and Spasmolytic activities

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Figure 1

HPLC chromatograms of three extracts of Bao-Xie-Ning and the standard mixture 169 mm×140 mm (96 × 96 DPI)"

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Figure 2

Inhibition rates of three extracts on spontaneous contractions of isolated duodenum in different concentrations EE: ethanolic extract; VOE: volatile oil extract; AE: aqueous extract."

Figure 3

Effects of three extracts on the contractile response of isolated duodenum pretreated by acetylcholine (ACH), BaCl2 or adrenalin (ADR) respectively. A: Ethanolic extract (EE) 32 mg/mL; B: Volatile oil extract (VOE) 12.56 mg/mL; C: Aqueous extract (AE) 90 mg/mL."

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