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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (8): 911-917.doi: 10.3736/jcim20120812

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Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy

Xin-min Li(), Hui Chen, Yan-yan Ren, Yan-li Lu#br#   

  1. Department of Paediatrics, the First Affiliated Hospital, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • Received:2011-11-09 Accepted:2011-11-21 Online:2012-08-20 Published:2018-08-15
  • Contact: Xin-min Li E-mail:lixinmin1998@sohu.com

Objective: To study the effects of Xifeng Capsule, a compound traditional Chinese herbal medicine, combined with carbamazepine on spontaneous epileptic seizure induced by lithium and pilocarpine in rats and the expression level of multidrug resistance-associated protein 1 (MRP1).

Methods: Lithium and pilocarpine were used to induce epilepsies in rats. All epileptic rats were randomly divided into model, high-dose Xifeng Capsule, medium-dose Xifeng Capsule, low-dose Xifeng Capsule, high-dose Xifeng Capsule plus carbamazepine (CBZ) (combined high-dose group), high-dose Xifeng Capsule plus half dose of CBZ (combined low-dose group) and CBZ groups with 10 rats in each group. And another 10 normal rats served as control. After treating 28 d, immunohistochemical method was used to detect the MRP1 expression in cortex and hippocampus of the epileptic rats.

Results: : MRP1 expression in hippocampus of the treated groups was higher than that of the normal control group, with wider range and darker positive particles, but was lower than that of the model group. In the cortical areas, the differences between the combined high-dose group or the combined low-dose group and the model group were statistically significant (P<0.05). Regardless of the hippocampus CA1, CA3, gyrus or cortical areas, the influence of high-dose Xifeng Capsule on MRP1 distribution was superior to that of low-dose Xifeng Capsule; Xifeng Capsule combined with CBZ had better effects than low-dose Xifeng Capsule, medium-dose Xifeng Capsule and CBZ used alone (P<0.05).

Conclusion: Xifeng Capsule used alone or combined with CBZ can effectively inhibit MRP1 expression in hippocampus and cortex of epileptic rats.

Key words: epilepsy, drugs, Chinese herbal, pilocarpine, multidrug resistance-associated protein 1, rats

Table 1

Effects of Xifeng Capsule and CBZ on SRS attack in rats with lithium-pilocarpine-induced epilepsy ($\bar{x}$±s)"

Group n Times of SRS
Model 9 35.7±4.2
Low-dose Xifeng Capsule 10 26.6±2.7△△
Medium-dose Xifeng Capsule 10 21.1±2.0△△▲□
High-dose Xifeng Capsule 10 13.5±3.7△△▲
20 mg CBZ 10 17.0±3.9△△□
High-dose Xifeng Capsule plus 20 mg CBZ 10 7.9±3.2△△▲□■
High-dose Xifeng Capsule plus 10 mg CBZ 10 5.5±2.1△△▲□■

Figure 1

Expression of MRP1 in cerebral cortex of rats observed by immunohistochemical method (Light microscopy, ×400) A: Normal control group; B: Model group. C: Low-dose Xifeng Capsule group; D: Medium-dose Xifeng Capsule group; E: High-dose Xifeng Capsule group; F: CBZ group; G: High-dose Xifeng Capsule plus 20 mg CBZ group; H: High-dose Xifeng Capsule plus 10 mg CBZ group. CBZ: carbamazepine; MRP1: multidrug resistance-associated protein 1. "

Figure 2

Expression of MRP1 in hippocampus of rats observed by immunohistochemical method (Light microscopy, ×400) A: Normal control group; B: Model group. C: Low-dose Xifeng Capsule group; D: Medium-dose Xifeng Capsule group; E: High-dose Xifeng Capsule group; F: CBZ group; G: High-dose Xifeng Capsule plus 20 mg CBZ group; H: High-dose Xifeng Capsule plus 10 mg CBZ group. CBZ: carbamazepine; MRP1: multidrug resistance-associated protein 1. "

Table 2

Effects of Xifeng Capsule and CBZ on distribution of MRP1 in rats with lithium-pilocarpine-induced epilepsy ($\bar{x}$±s)"

Group n Expression of MRP1
Cerebral cortex CA1 region CA3 region Dentate gyrus
Normal control 6 0.33±0.18 0.16±0.07 1.48±0.35 2.00±0.36
Model 6 4.00±0.82* 11.75±2.43* 10.25±6.97* 12.02±1.64*
Low-dose Xifeng Capsule 6 3.37±0.39* 8.45±1.02*△ 7.07±1.82* 10.72±1.97*
Medium-dose Xifeng Capsule 6 3.07±0.41*△□☆ 4.82±2.22*△□☆ 6.97±0.81*□☆ 10.07±0.89*△□☆
High-dose Xifeng Capsule 6 2.28±0.28*△▲ 1.85±0.45*△▲ 3.05±0.46*△▲ 4.70±0.91*△▲
20 mg CBZ 6 2.53±0.86*△▲ 2.60±0.87*△ 3.02±0.26*△ 6.10±0.72*△□
High-dose Xifeng Capsule plus 10 mg CBZ 6 2.02±0.67*△▲ 1.55±0.61*△▲■ 2.32±0.35*△▲□■☆ 3.10±0.49*△▲□■
High-dose Xifeng Capsule plus 20 mg CBZ 6 1.17±0.74*△▲□■ 1.05±0.27*△▲□■ 1.90±0.15*△▲□■ 2.60±0.54*△▲□■
[1] Shen DL, Wang XF. Clinical epileptology.2nd ed[M]. Shanghai: Shanghai Scientific and Technical Publishers, 2007,403
沈鼎列, 王学峰 . 临床癫痫学.第2版[M]. 上海: 上海科学技术出版社, 2007,403
[2] Li R, Huang YG . P-glycoprotei and intractable epilepsy[J]. Cu Zhong Yu Shen Jing Ji Bing, 2003,10(5):315-316
doi: 10.3969/j.issn.1007-0478.2003.05.025
李锐, 黄远桂 . P糖蛋白与难治性癫痫[J]. 卒中与神经疾病, 2003,10(5):315-316
doi: 10.3969/j.issn.1007-0478.2003.05.025
[3] Ma R, Li XM, Wei XW, Zhang XL, Yang CQ, Xiong J, Sun XH, Liu YZ . The study of Xifeng capsule on epilepsy tonic-clonic seizure in epileptic children[J]. Tianjin Zhong Yi Yao, 2003,20(2):87
马融, 李新民, 魏小维, 张喜莲, 杨常泉, 熊杰, 孙希焕, 刘玉珍 . 熄风胶囊治疗小儿癫痫强直-阵挛性发作的研究[J]. 天津中医药, 2003,20(2):87
[4] Wang BG, Liao WP, Luo AH, Sun WW, Su T . Comparison study of behavioral and histological changes between pilocarpine-induced epilepsy model and pentylenetetrazole kindling model in rats[J]. Lin Chuang He Shi Yan Yi Xue Za Zhi, 2006,5(3):197-199
王本国, 廖卫平, 罗爱华, 孙卫文, 苏涛 . 匹罗卡品癫痫模型及戊四唑点燃模型的行为学、组织学对比研究[J]. 临床和实验医学杂志, 2006,5(3):197-199
[5] Mazarati AM, Telegdy G . Effects of somatostatin and anti-somatostatin serum on picrotoxin-kindled seizures[J]. Neuropharmacology, 1992,31(8):793-797
doi: 10.1016/0028-3908(92)90043-O pmid: 1356254
[6] Xiong J, Ma R, Yang CQ, Zhang GZ . The effect of Xifeng Capsule on the activity of nitric oxide synthase in hippocampal subareas of epileptic mice[J]. Shanxi Zhong Yi, 2003,4(2):52-53
熊杰, 马融, 杨常泉, 张果忠 . 熄风胶囊对癫痫小鼠海马一氧化氮合酶活力的影响[J]. 山西中医, 2003,4(2):52-53
[7] Sisodiya SM, Lin WR, Harding BN, Squier MV, Thom M . Drug resistance in epilepsy: expression of drug resistance proteins in common causes of refractory epilepsy[J]. Brain, 2002,125(Pt 1):22-31
doi: 10.1093/brain/awf002
[8] Seegers U, Potschka H, Löscher W . Expression of the multidrug transporter P-glycoprotein in brain capillary endothelial cells and brain parenchyma of amygdala-kindled rats[J]. Epilepsia, 2002,43(7):675-684
doi: 10.1046/j.1528-1157.2002.33101.x
[9] Rizzi M, Caccia S, Guiso G, Richichi C, Gorter JA, Aronica E, Aliprandi M, Bagnati R, Fanelli R , D’Incalci M , Samanin R , Vezzani A .Limbic seizures induce P-glycoprotein in rodent brain: functional implications for pharmacoresistance[J]. J Neurosci, 2002,22(14):5833-5839
doi: 10.1523/JNEUROSCI.22-14-05833.2002
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