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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (12): 1286-1289.doi: 10.3736/jcim20081215

• Original Experimental Research • Previous Articles     Next Articles

Extraction of praeruptorin A from Radix Peucedani by supercritical fluid extaction-CO2

Ai-dong Liu1, Wei Hou2, Xue-song Chen3, Zhi-dong Qiu4()   

  1. 1. Department of Cardiology, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, Jilin Province 130021, China
    2. Institute of Special Wild Economic Animal and Plant, Chinese Academy of Agricultural Sciences, Changchun, Jilin Province 132109, China
    3. Department of Research and Development, Chuangzhi Medical Development Company Limited, Jilin Province, Changchun, Jilin Province 130051, China
    4. Department of Pharmaceutics, College of Pharmacy, Changchun University of Traditional Chinese Medicine, Changchun, Jilin Province 130117, China
  • Received:2008-04-20 Online:2008-12-20 Published:2008-12-15

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Objective:

To extract praeruptorin A from Radix Peucedani by supercritical fluid extraction (SFE)-CO2.


Methods: 

After preliminary experiment, three main factors were acquired that could influence the result of SFE-CO2, including the time, pressure and temperature of the extraction. The optimal extraction process was carried out on orthogonal design, and SFE-CO2 was compared with the traditional methods.


Results: 

In the extraction of the praeruptorin A, the best extraction conditions were 60 ℃, 20 MPa, and duration for three hours. As cosolvent alcohol was added, the amount of extraction of the praeruptorin A increased, and the amount of SFE-CO2 extraction was higher than those of decoction and heating reflux.


Conclusion:

The SFE-CO2 in extracting praeruptorin A from Radix Peucedani is feasible and reliable.

Key words: chromatography, supercritical fluid, Peucedanum decursivum, praeruptorin A

CLC Number: 

  • R284.2

Table 1

Factors and levels in SFE-CO2 method"

Level Factor
A: Extract temperature (℃) B: Extract pressure (MPa) C: Extract time (h)
1 40 20 1
2 50 30 2
3 60 40 3

Table 2

Result of orthogonal experiment"

No. A B C D (blank) Extraction of praeruptorin A (mg)
1 1 1 1 1 7.78
2 1 2 2 2 13.08
3 1 3 3 3 16.27
4 2 1 2 3 15.63
5 2 2 3 1 19.45
6 2 3 1 2 13.30
7 3 1 3 2 22.27
8 3 2 1 1 15.66
9 3 3 2 3 17.31
Ⅰj 37.13 45.68 36.74 44.54
Ⅱj 48.38 48.19 46.02 48.65
Ⅲj 55.24 46.88 57.99 47.56

Table 3

Result of analysis of variance"

Source SS Degree of freedom Mean square F P
A 6.036 665 2 55.732 7 53.036 47 <0.05
B 0.836 667 2 1.050 8 2.876 22 >0.05
C 7.083 333 2 75.662 6 72.002 18 <0.05
D 1.370 000 2 3.022 4 1 >0.05
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