To study the effects of Gegen Recipe and Guizhi Recipe regulating the expression of IL-1β, iNOS, TNFα and TGFβ mRNA in degenerated intervertebral disk caused by stimulation of wind, cold and damp.
Thirty-six rabbits were randomly separated into six groups, viz. control group, stimulation by wind, cold and damp group, head in low-location group, compound model group(head in low-location plus stimulation of wind, cold and damp), Gegen Recipe group and Guizhi Recipe group. The gene expression of IL-1β, iNOS, TNFα and TGFβ mRNA in degenerated cervical intervertebral disks were assayed by RT-PCR.
Compared with the control group, IL-1β mRNA and iNOS mRNA expression were up-regulated in each model group (P<0.01). Both Gegen Recipe and Guizhi Recipe down-regulated IL-1β mRNA, iNOS mRNA expression, which had significant differences compared with the stimulation model group (P<0.01 or<0.05). TNFα mRNA expression was significantly enhanced in the compound model group compared with the control group (P<0.01). There was no significant difference between the head in low-location group and the stimulation model group. Gegen Recipe down-regulated TNFα mRNA expression compared with the stimulation group (P<0.01), while the effect of Guizhi Recipe was not significant. Compared with the control group, TGFβ mRNA expression went down in each model group (P<0.01). Gegen Recipe up-regulated TGFβ mRNA expression (P<0.01), while the effect of Guizhi Recipe on adjusting TGFβ mRNA was not significant.
Stimulation by wind, cold and damp, head in low-location, and compound of both can down-regulate the expressions of IL-1β, iNOS, TNFα mRNA, and up-regulate the expression of TGFβ mRNA. These researches certify that these factors take part in the progression of intervertebral disk degeneration. IL-1β, iNOS, TNFα accelerate the degeneration and TGFβ defers the degeneration. Gegen Recipe down-regulates the expression of TNFα mRNA and up-regulates the expression of TGFβ mRNA. The effect on regulating the cell factors expressions by Guizhi Recipe is lower than that of Gegen Recipe.