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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (4): 273-276.doi: 10.3736/jcim20030413

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Regulation effects of Gegen recipe and Guizhi recipe on expression of IL-1β, iNOS, TNFα, TGFβ mRNA in degenerated cervical intervertebral disk in rabbits

Yong-jun Wang, Qi Shi, Chong-jian Zhou, Bao-xing Hou, Mei Liu, Wen Mo   

  1. Institute of Spinal Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2003-09-04 Online:2003-12-20 Published:2003-12-20


To study the effects of Gegen Recipe and Guizhi Recipe regulating the expression of IL-1β, iNOS, TNFα and TGFβ mRNA in degenerated intervertebral disk caused by stimulation of wind, cold and damp.


Thirty-six rabbits were randomly separated into six groups, viz. control group, stimulation by wind, cold and damp group, head in low-location group, compound model group(head in low-location plus stimulation of wind, cold and damp), Gegen Recipe group and Guizhi Recipe group. The gene expression of IL-1β, iNOS, TNFα and TGFβ mRNA in degenerated cervical intervertebral disks were assayed by RT-PCR.


Compared with the control group, IL-1β mRNA and iNOS mRNA expression were up-regulated in each model group (P<0.01). Both Gegen Recipe and Guizhi Recipe down-regulated IL-1β mRNA, iNOS mRNA expression, which had significant differences compared with the stimulation model group (P<0.01 or<0.05). TNFα mRNA expression was significantly enhanced in the compound model group compared with the control group (P<0.01). There was no significant difference between the head in low-location group and the stimulation model group. Gegen Recipe down-regulated TNFα mRNA expression compared with the stimulation group (P<0.01), while the effect of Guizhi Recipe was not significant. Compared with the control group, TGFβ mRNA expression went down in each model group (P<0.01). Gegen Recipe up-regulated TGFβ mRNA expression (P<0.01), while the effect of Guizhi Recipe on adjusting TGFβ mRNA was not significant.


Stimulation by wind, cold and damp, head in low-location, and compound of both can down-regulate the expressions of IL-1β, iNOS, TNFα mRNA, and up-regulate the expression of TGFβ mRNA. These researches certify that these factors take part in the progression of intervertebral disk degeneration. IL-1β, iNOS, TNFα accelerate the degeneration and TGFβ defers the degeneration. Gegen Recipe down-regulates the expression of TNFα mRNA and up-regulates the expression of TGFβ mRNA. The effect on regulating the cell factors expressions by Guizhi Recipe is lower than that of Gegen Recipe.

Key words: drugs, Chinese herbal, cervical vertebrae, intervertebral disk, interleukin-1beta, nitric-oxide synthase, tumor necrosis factor-alpha, etc

CLC Number: 

  • R681.531


引物名称,片段(bp) 引物序列
IL-1β(305 bp) Forward
iNOS(231 bp) Forward
TGFβ(250 bp) Forward
TNFα(340 bp) Forward
β-actin(660 bp) Forward


组 别 n IL-1β mRNA iNOS mRNA
正常对照组 6 0.9532±0.0047 0.9199±0.0129
风寒湿颈椎病组 6 1.0413±0.0152** 1.0026±0.0096**
低头位颈椎病组 6 1.0297±0.0313** 1.0049±0.0182**
低头位加风寒湿颈椎病组 6 1.0885±0.0271**△▲▲ 1.0160±0.0078**
葛根汤治疗组 6 0.9908±0.0121 0.9437±0.0031△△
桂枝汤治疗组 6 0.9529±0.0025△△ 0.9057±0.0044△△


组 别 n TNFα mRNA TGFβ mRNA
正常对照组 6 0.8906±0.0211 0.9710±0.0572
风寒湿颈椎病组 6 0.9045±0.0199 0.8384±0.0305**
低头位颈椎病组 6 0.8958±0.0169 0.8254±0.0351**
低头位加风寒湿颈椎病组 6 1.0284±0.0182**△△▲▲ 0.8027±0.0212**
葛根汤治疗组 6 0.8578±0.0294△△ 0.9662±0.0127△△
桂枝汤治疗组 6 0.9819±0.0285△△ 0.8679±0.0158□□
[1] Rand N, Reichert F, Floman Y , et al. Murine nucleus pulposus-derived cells secrete interleukins-1-beta, -6, and 10 and granulocyte-macrophase colony-stimulating factor in cell culture[J]. Spine, 1997,22(22):2598-2602
doi: 10.1097/00007632-199711150-00002
[2] Taskiran D, Stefanovic-Racic M, Georgescu H , et al. Nitric oxide mediates suppression of cartilage proteoglycan synjournal by interleukin-1[J]. Biochem Biophys Res Commun, 1994,200(1):142-148
doi: 10.1006/bbrc.1994.1426 pmid: 7513156
[3] Kawakami M, Tamaki T, Weinstein JN , et al. Pathomechanism of pain-related behavior produced by allografts of intervertebral disc in the rat[J]. Spine, 1996,21(18):2101-2107
doi: 10.1097/00007632-199609150-00009 pmid: 8893434
[4] Black RA, Rauch CT, Kozlosky CJ , et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells[J]. Nature, 1997,385(6618):729-733
doi: 10.1038/385729a0
[5] Viviani B, Corsini E, Galli CL , et al. Glia increase degeneration of hippocampal neurons through release of tumor necrosis factor-alpha[J]. Toxicol Appl Pharmacol, 1998,150(2):271-276
doi: 10.1038/sj.ejhg.5201682 pmid: 9653057
[6] Ignatowski TA, Covey WC, Knight PR , et al. Brain-derived TNF alpha mediates neuropathic pain[J]. Brain Res, 1999,841(1-2):70-77
doi: 10.1016/S0006-8993(99)01782-5
[7] Nishida K, Kang JD, Gilbertson LG , et al. Modulation of the biologic activity of the rabbit intervertebral disc by gene therapy:an in vivo study of adenovirus-mediated transfer of the human transforming growth factor beta 1 encoding gene[J]. Spine, 1999,24(23):2419-2425
doi: 10.1097/00007632-199912010-00002
[8] 贺玉琢, 富杭育, 周爱香 , 等. 经抗炎的药效法再探麻黄汤、桂枝汤、银翘散、桑菊饮的药物动力学[J]. 中药药理与临床, 1993,9(1):1-3
[9] 富杭育, 贺玉琢, 周爱香 , 等. 经解热的药效法初探麻黄汤、桂枝汤、银翘散、桑菊饮的药物动力学[J]. 中药药理与临床, 1992,8(1):1-3
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