[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 123-125.doi: 10.3736/jcim20040214

• Original Experimental Research • Previous Articles     Next Articles

An animal model of alcoholic fatty liver and the prevention and treatment with New Qinggan Decoction

Guo-jie Hu1, Dian-gui Li2, Shi-fang Li1, Zhen-bin Li3, Jun-xia Li3   

  1. 1. Department of Infectious Diseases, General Hospital of Huabei Oil Field,Renqiu,Hebei Province 062552, China
    2. Hebei Staff Medical College, Baoding, Hebei Province 071000, China
    3. Department of Traditional Chinese Medicine, Bethune International Peace Hospital, Shijiazhuang, Hebei Province 050073, China
  • Online:2004-03-20 Published:2018-10-26

RichHTML

0

PDF (PC)

415

Like

Objective: To establish an animal model of alcoholic fatty liver (AFL) and to observe the prevention and treatment with New Qinggan Decoction (NQGD). Methods: Female SD rats were exposed to the mixture of white spirit and corn oil continuously for up to 6 weeks via an intragastric feeding protocol. The pathologic alteration of the liver and the changes of serum lipid were observed, and the effect of NQGD for preventing and treating AFL was studied. Results: Wide hepatic steatosis was induced in model group rats. Serum triglyceride (TG) and total cholesterol (TC) of the model group were higher than those of the normal group (P＜0.01). Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of the model group were higher than those of the normal group (P<0.05). In NQGD group, hepatic steatosis was milder than that in the model group, and serum TG, TC, AST, ALT descended significantly as compared with the model group (P＜0.01). Conclusion: AFL model of rats established by feeding the mixture of white spirit and corn oil is similar to the episode of the human AFL, and is easy to intervene with medicine. NQGD can prevent the occurrence of AFL in this model.

Key words: alcoholic fatty liver, animal model, rat, New Qinggan Decoction

CLC Number: 

  • R575.5

Figure 1

Histomorphological examination of SD rat liver tissue (HE staining,×200)A: Normal group; B: Model group; C: NQGD low dose group; D: NQGD high dose group"

Tab 1

Serum TG and TC of rats in 4 groups (ヌ±S, mmol/L)"

Group n TG TC
Normal 8 0.77±0.12 1.84±0.14
Model 8 1.19±0.30** 2.21±0.31**
NQGD high dose 8 0.74±0.17△△ 1.67±0.19△△
NQGD low dose 8 0.79±0.27△△ 1.68±0.22△△

Tab 2

Serum AST and ALT of rats in 4 groups (ヌ±S ,mmol/L)"

Group n AST ALT
Normal 8 204.85±46.23 36.92±4.43
Model 8 241.67±23.88* 43.71±4.42*
NQGD high dose 8 171.09±24.44△△ 32.61±4.84△△
NQGD low dose 8 193.98±32.90△△ 36.20±4.38△△
[1] 叶永安, 田德录, 赵凤志 , 等. 慢肝消对大鼠酒精性肝损伤防护作用的研究[J]. 中国中医基础医学杂志, 1996,2(6):32-33
[2] 河福金, 贲长恩, 王德福 . 小柴胡汤对大鼠酒精性肝损伤的防护作用[J]. 中西医结合肝病杂志, 1994,4(3):19-21
[3] 江正辉, 王泰龄 . 酒精性肝病(第1版)[M]. 北京: 中国医药科技出版社,2001,(第1版): 42, 100-101
[4] 赵静波, 王泰龄, 张晶 , 等. 大鼠急性酒精性肝损伤模型分析[J]. 中日友好医院学报, 1996,10(1):17-19
[5] 李舒丹, 厉有名, 虞朝辉 . 大鼠慢性酒精性肝损伤模型的建立[J]. 浙江医学, 2002,24(9):524-525, 530
doi: 10.3969/j.issn.1006-2785.2002.09.005
[6] 王新月, 孙承琳, 田德禄 , 等. 解酒口服液对小鼠酒精性肝损伤的防护作用机理研究[J]. 中医杂志, 2000,41(12):748-750
[7] 李育浩, 邓响嘲, 吴清和 , 等. 五子衍宗丸对乙醇性肝损伤大鼠脂质代谢的影响[J]. 中国中药杂志, 1994,19(5):300-302
[8] 郑淑芳, 郑纪宁, 赵淑萍 , 等. 酒精中毒性肝损伤的体视学研究[J]. 河北医科大学学报, 2002,23(2):81-83
[9] Gardner CD, Kraemer HC . Monounsaturated versus polyunsaturated dietary fat and serum lipids. A meta-analysis[J]. Arterioscler Thromb Vasc Biol, 1995,15(11):1917-1927
[10] 李东良, 郭晓萍, 邓岚 , 等. 试论酒精性肝病的病因病机及治法[J]. 中医药学报, 1996,3(4):9-10
[11] 郑虎占, 董泽宏, 佘靖 . 中药现代药理研究与应用(第1版)[M]. 北京: 学苑出版社, 1998,( 第1版):4316-4317
[12] 吴葆杰 . 中草药药理学(第1版)[M]. 北京: 人民卫生出版社, 1983,( 第1版):15
[1] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[2] Lawrence A. Olatunji, Taofeek O. Usman, Joseph O. Adebayo, Victoria A. Olatunji . Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats. Journal of Chinese Integrative Medicine, 2012, 10(9): 1049-1055.
[3] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[4] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[5] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[6] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[7] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[8] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[9] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[10] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[11] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[12] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[13] Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
[14] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
[15] Hui-qing Zhang , Zi-fei Yin , Jia-yu Sheng , Zhu-qian Jiang , Bao-yu Wu , Yong-hua Su. A comparison study of pharmacokinetics between bufalin-loaded bovine serum albumin nanoparticles and bufalin in rats. Journal of Chinese Integrative Medicine, 2012, 10(6): 674-680.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]