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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 111-114.doi: 10.3736/jcim20050209

• Original Experimental Research • Previous Articles     Next Articles

Effects of Yiqi Huayu Recipe on gene expression pattern of normal and apoptotic chondrocytes in cervical intervertebral disc in rats

Yong-jun Wang1,Peng Sun2,Quan Zhou1,Mei Liu1,Zhi-jun Hu1,Chong-jian Zhou1,Qi Shi1   

  1. 1. Spinal Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. College of Acupuncture and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2004-09-20 Online:2005-03-20 Published:2005-03-20
  • Contact: Yong-jun Wang E-mail:yjwang88@hotmail.com

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Objective

To study the effects of Yiqi Huayu Recipe on the gene expression pattern in normal and apoptotic chondrocytes in the cervical intervertebral disc of rats.

Methods

The intervertebral disc endplates of rats were digested enzymatically. The apoptosis of the chondrocytes was induced by anti-Fas antibody. BiostarR-40s microarray chips were used to investigate the gene expression pattern in chondrocytes in the normal control, apoptosis and Yiqi Huayu Recipe groups. The results were scanned with Scan Array 4000 and analyzed with GenePix Pro 3.0, and then subjected to standardization and ratio analysis.

Results

In the apoptosis group, 30 kinds of genes expressed in cervical intervertebral disc chondrocytes were screened out, in which 10 were up-regulated (ratio>2) and 20 down-regulated (ratio<0.5) as compared with the normal control group. On the other hand, in the Yiqi Huayu Recipe group, 97 kinds of genes expressed in cervical intervertebral disc chondrocytes were screened out, in which 44 were up-regulated (ratio>2) and 53 down-regulated (ratio<0.5) as compared with the apoptosis group.

Conclusion

There are some signal transduction pathways that control the apoptosis of the intervertebral disc chondrocytes. Yiqi Huayu Recipe regulates the gene express of the apoptotic chondrocytes in intervertebral disc. The study gives a further understanding to the mechanism of the cervical spondylosis and enriches the theories of qi and blood of traditional Chinese medicine.

Key words: Yiqi Huayu Recipe, Intervertebral disc, Chondrocytes, Apoptosis, Gene chip, Signal transduction

CLC Number: 

  • R289.5

Tab 1

mRNA preparation result"

Group mRNA result （μg） OD260/OD280
Normal control 23.04 2.09
Apoptosis 84.72 1.97
Yiqi Huayu Recipe 146.64 2.05

Fig 1

Gene chip hybridized signal of normal control and apoptosis groupsA: Fluorescent marked overlay; B: Scatter"

Fig 2

Gene chip hybridized signal of Yiqi Huayu Recipe and apoptosis groups A: Fluorescent marked overlay; B: Scatter"

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