蒲公英萜醇和乙酰蒲公英萜醇对胃癌细胞株AGS细胞周期和凋亡的影响

doi:10.3736/jcim20110610

Abstract

Objective: To investigate the effects of taraxerol and taraxeryl acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS cells.
Methods: The inhibitory effects of taraxerol and taraxeryl acetate at different concentrations on AGS cell growth were measured by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and the concentrations of taraxerol and taraxeryl acetate to be used in following experiments were decided. Then, cell cycle analysis was performed by FACScan flow cytometry after culture with taraxerol or taraxeryl acetate. Annexin V-fluorescein isothiocyanate/propidium iodide staining was used to measure cell apoptosis.
Results: Taraxerol significantly inhibited AGS cell proliferation in a dose- and time-dependent manner. Taraxerol arrested the AGS cells at G₂/M stage. 110 μmol/L taraxerol elevated the population of AGS cells arrested in G₂/M phase compared with solvent (P<0.05). Taraxerol also promoted early cell apoptosis in AGS cells. 110 μmol/L taraxerol increased the early cell apoptosis rate from 4.45% to 10.29%, which was 1.31 times higher than that of the untreated cells. However, taraxeryl acetate had a lower inhibitory effect than taraxerol, and it showed a tendency of G₂/M arrest and apoptosis promotion but with no statistical significance (P>0.05).
Conclusion: Taraxerol has inhibitory effects on AGS cell growth through inducing G₂/M arrest and promotion of cell apoptosis. Taraxeryl acetate has less effect on cell cycle arrest and apoptosis of AGS cells than taraxerol.

Key words: Taraxacum mongolicum , drugs,Chinese herbal, gastric cancer, apoptosis, cell cycle

Bao Tan, Hai-lian Shi, Guang Ji, Jian-qun Xie. Effects of taraxerol and taraxeryl acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS. Journal of Chinese Integrative Medicine, 2011, 9(6): 638-642.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20110610

http://www.jcimjournal.com/EN/Y2011/V9/I6/638

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Effects of taraxerol and taraxeryl acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS

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