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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 299-302.doi: 10.3736/jcim20050415

• Original Experimental Research • Previous Articles     Next Articles

Effects of Anxin Granules on blood lipid and ultrastructure of aorta in rabbits with dyslipidemia

Xian-ming Fang1,Ying Yuan1,Yun-hong Wu1,Xue-man Yin2   

  1. 1. Department of Cardiovascular Internal Medicine, Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning, Guangxi Province 530011, China
    2. Department of Pathology, Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning, Guangxi Province 530011, China
  • Received:2004-11-08 Online:2005-07-20 Published:2005-07-20
  • Contact: Xian-ming Fang E-mail:fxm621@126.com

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Objective

To study the effects of Anxin Granules on dyslipidemia in rabbits caused by high fat plus high cholesterol diet.

Methods

Thirty-two healthy New Zealand male white rabbits were randomly divided into 4 groups: normal control group, untreated group, Zhibituo Tablet-treated group and Anxin Granule-treated group. Rabbits in the normal control group were fed with regular chow, while rabbits in the other three groups were fed with high fat plus high cholesterol diet. Zhibituo Tablets and Anxin Granules were administered to the rabbits in Zhibituo Tablet-treated group and Anxin Granule-treated group at a daily oral dose respectively. At the end of the 10th week, the levels of serum total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB) were tested in each group, and the ultrastructures of the aorta were also observed by an electron microscope.

Results

Anxin Granules could reduce the levels of TC, TG, LDL-C and ApoB. The results observed by electron microscope showed that, as compared with the untreated group and the Zhibituo Tablet-treated group, the atherosclerosis of aorta in the Anxin Granule-treated group was lighter. And it was found that there were few lipid droplet vacuoles in cytoplasm of the endothelial cells, and various cell organs and elastic membrane were existed, but no lipid droplet vacuoles in cytoplasm of the medial smooth muscle cells.

Conclusion

Anxin Granules can regulate the metabolism of blood lipid and inhibit the formation of atherosclerosis caused by hyperlipidemia in rabbits.

Key words: Anxin Granules, Dyslipidemia, Models, Animal, Aorta, Ultrastructure

CLC Number: 

  • R589.21

Tab 1

Levels of TC, TG, HDL-C and LDL-C before and after administration in 4 groups ?$\bar{x}$±s, mmol/L)"

Group n TC TG HDL-C LDL-C
Normal control group
Before administration 8 1.55±0.41 1.39±0.42 0.61±0.09 0.35±0.30
After administration 8 1.52±0.79 0.74±0.07 0.51±0.09 0.68±0.75
Untreated group
Before administration 8 1.68±0.48 1.28±0.49 0.66±0.10 0.63±0.35
After administration 8 24.15±2.78** 2.42±1.03** 0.82±0.10 22.09±1.31**
Zhibituo Tablet group
Before administration 8 1.46±0.19 1.47±0.32 0.67±0.14 0.52±0.51
After administration 8 16.34±4.17△△ 0.74±0.22△△ 0.66±0.04 15.21±3.94△△
Anxin Granule group
Before administration 8 1.45±0.37 1.18±0.13 0.64±0.22 0.58±0.50
After administration 8 9.65±4.37△△▲▲ 0.83±0.19△△ 0.54±0.07 7.65±2.47△△▲▲

Tab 2

Levels of ApoA1, ApoB and ApoA1/ApoB before and after administration in 4 groups ($\bar{x}$±s)"

Group n ApoA1（mmol/L) ApoB （mmol/L) ApoA1/ApoB
Normal control group
Before administration 8 0.36±0.37 0.17±0.04 2.24±0.37
After administration 8 0.37±0.11 0.19±0.03 2.06±0.75
Untreated group
Before administration 8 0.41±0.32 0.22±0.19 2.26±0.35
After administration 8 0.96±0.22 1.34±0.10** 0.72±0.07**
Zhibituo Tablet group
Before administration 8 0.39±0.25 0.18±0.25 2.37±0.24
After administration 8 0.67±0.18 1.10±0.65 0.70±0.22
Anxin Granule group
Before administration 8 0.38±0.28 0.18±0.11 2.21±0.16
After administration 8 0.42±0.10 0.54±0.14△△ 0.80±0.15

Fig 1

Ultrastructure of rabbit aorta observed by electron microscope (1) A, B: Normal control group (×5000); C: Untreated group (×7500)"

Fig 2

Ultrastructure of rabbit aorta observed by electron microscope (2) A: Zhibituo Tablet-treated group (×7500); B, C: Anxin Granule-treated group (×10000)"

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