Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (2): 209-215.doi: 10.3736/jcim20110215

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Yinsiwei compound on spatial learning and memory ability and the ultrastructure of hippocampal neurons in a rat model of sporadic Alzheimer disease

 Yong-chang Diwu1,2(), Jin-zhou Tian1Shi Jing1   

  1. 1. First Clinical Medical College,Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China
    2. Clinical Medical College of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China
  • Received:2010-10-17 Accepted:2010-11-08 Online:2011-02-20 Published:2011-02-15
  • Contact: Diwu Yong-chang E-mail:ycdw07@yahoo.com.cn

Objective: To study the effects of Chinese herbal medicine Yinsiwei compound (YSW) on spatial learning and memory ability in rats with sporadic Alzheimer disease (SAD) and the ultrastructural basis of the hippocampal neurons.
Methods: A rat model of SAD was established by intracerebroventricular injection of streptozotocin. The rats were divided into six groups: sham-operation group, model group, donepezil control group, and YSW low, medium and high dose groups. Drug interventions were started on the 21st day after modeling and each treatment group was given the corresponding drugs by gavage for two months. Meanwhile, the model group and the sham-operation group were given the same volume of distilled water by gavage once a day for two months. The Morris water maze was adopted to test spatial learning and memory ability of the rats. The place navigation test and the spatial probe test were conducted. The escape latency, total swimming distance and swimming time in the target quadrant of the rats were recorded. Also, the hippocampus tissues of rats were taken out and the ultrastructure of hippocampus neurons were observed by an electron microscope.
Results: In the place navigation test, compared with the model group, the mean escape latency and the total swimming distance of the donepezil group and the YSW low, medium and high dose groups were significantly shortened (P<0.05 or P<0.01). In the space probe test, the swimming time of each treatment group in the target quadrant was significantly longer than that of the model group (P<0.05 or P<0.01). For most of the test period, the donepezil group had no significant change compared with the YSW low, medium and high dose groups, respectively. The ultrastructure of the hippocampus neurons under the electron microscope also confirmed the efficacy of the drug treatment.
Conclusion: Chinese herbal medicine YSW compound can improve spatial learning and memory impairment of rats with SAD. The ultrastructural basis may be that it can protect the microtubule structures of hippocampal neurons and prevent nerve axons from being damaged.

Key words: Alzheimer disease, drugs,Chinese herbal, maze learning, learning disorders, memory disorders, hippocampus, animal experimentation, rats

"

Figure 1

Comparison of average escape latency in the place navigation test of rats in each group YL: Yinsiwei compound of low dose; YM: Yinsiwei compound of medium dose; YH: Yinsiwei compound of high dose."

"

Figure 2

Comparison of swimming distance in the place navigation test of rats in each group YL: Yinsiwei compound of low dose; YM: Yinsiwei compound of medium dose; YH: Yinsiwei compound of high dose."

"

Figure 3

Microtubule structure of the hippocampal neuronal axons of rats in each group A: Sham-operation group (Electron microscopy, ×60 000); B: Model group (Electron microscopy, ×60 000); C: Donepezil group (Electron microscopy, ×50 000); D: Yinsiwei high dose group (Electron microscopy, ×50 000); E: Yinsiwei medium dose group (Electron microscopy, ×60 000); F: Yinsiwei low dose group (Electron microscopy, ×50 000)."

[1] Lobo A, Launer LJ, Fratiglioni L, Andersen K, Di Carlo A, Breteler MM, Copeland JR, Dartigues JF, Jagger C, Martinez-Lage J, Soininen H , Hofman A; the Neurologic Diseases in the Elderly Research Group.Prevalence of dementia and major subtypes in Europe: A collaborative study of population-based cohorts[J]. Neurology, 2000,54(11 Suppl 5):S4-S9
doi: 10.1212/WNL.54.1.4 pmid: 10636116
[2] Moceri VM, Kukull WA , Emanual I, van Belle G , Starr JR, Schellenberg GD, McCormick WC, Bowen JD, Teri L, Larson EB.Using census data and birth certificates to reconstruct the early-life socioeconomic environment and the relation to the development of Alzheimer’s disease[J]. Epidemiology, 2001,12(4):383-389
doi: 10.1097/00001648-200107000-00007
[3] Sharma M, Gupta YK . Intracerebroventricular injection of streptozotocin in rats produces both oxidative stress in the brain and cognitive impairment[J]. Life Sci, 2001,68(9):1021-1029
doi: 10.1016/S0024-3205(00)01005-5
[4] Tiwari V, Kuhad A, Bishnoi M, Chopra K . Chronic treatment with tocotrienol, an isoform of vitamin E, prevents intracerebroventricular streptozotocin-induced cognitive impairment and oxidative-nitrosative stress in rats[J]. Pharmacol Biochem Behav, 2009,93(2):183-189
doi: 10.1016/j.pbb.2009.05.009
[5] Hoyer S, Lannert H . Long-term effects of corticosterone on behavior, oxidative and energy metabolism of parietotemporal cerebral cortex and hippocampus of rats: comparison to intracerebroventricular streptozotocin[J]. J Neural Transm, 2008,115(9):1241-1249
doi: 10.1007/s00702-008-0079-7
[6] Ishrat T, Parveen K, Khan MM, Khuwaja G, Khan MB, Yousuf S, Ahmad A, Shrivastav P, Islam F . Selenium prevents cognitive decline and oxidative damage in rat model of streptozotocin-induced experimental dementia of Alzheimer’s type[J]. Brain Res, 2009,1281:117-127
doi: 10.1016/j.brainres.2009.04.010
[7] Paxinos G, Waston C. Translated by Zhuge QC.The rat brain in stereotaxic coordinates(3rd ed)[M]. Beijing: People’s Medical Publishing House, 2005
Paxinos G, Waston C. 诸葛启钏, 译.大鼠脑立体定向图谱(第3版)[M]. 北京: 人民卫生出版社, 2005
[8] Li AP, Zhao H, Li S, Piao H, Sun CK . Species differences of muridae in the learning and memory behavior in morris water maze[J]. Zhongguo Xing Wei Yi Xue Ke Xue, 2005,14(1):29-31
doi: 10.3760/cma.j.issn.1674-6554.2005.01.011
李爱萍, 赵慧, 李韶, 朴花, 孙长凯 . 不同鼠种在Morris水迷宫学习记忆行为中的种属差异[J]. 中国行为医学科学, 2005,14(1):29-31
doi: 10.3760/cma.j.issn.1674-6554.2005.01.011
[9] Riley KP, Snowdon DA, Markesbery WR . Alzheimer’s neurofibrillary pathology and the spectrum of cognitive function: findings from the Nun Study[J]. Ann Neurol, 2002,51(5):567-577
doi: 10.1002/(ISSN)1531-8249
[10] Gong CX, Liu F, Grundke-Iqbal I, Iqbal K . Post-translational modifications of tau protein in Alzheimer’s disease[J]. J Neural Transm, 2005,112(6):813-838
doi: 10.1007/s00702-004-0221-0
[11] Blokland A, Jolles J . Spatial learning deficit and reduced hippocampal ChAT activity in rats after an ICV injection of streptozotocin[J]. Pharmacol Biochem Behav, 1993,44(2):491-494
doi: 10.1016/0091-3057(93)90497-H
[12] de la Monte SM, Wands JR . Review of insulin and insulin-like growth factor expression, signaling, and malfunction in the central nervous system: relevance to Alzheimer’s disease[J]. J Alzheimers Dis, 2005,7(1):45-61
doi: 10.3233/JAD-2005-7106
[13] Sonkusare S, Srinivasan K, Kaul C, Ramarao P . Effect of donepezil and lercanidipine on memory impairment induced by intracerebroventricular streptozotocin in rats[J]. Life Sci, 2005,77(1):1-14
doi: 10.1016/j.lfs.2004.10.036
[14] Morris RG, Garrud P, Rawlins JN , O’Keefe J .Place navigation impaired in rats with hippocampal lesions[J]. Nature, 1982,297(5868):681-683
doi: 10.1038/297681a0 pmid: 7088155
[15] Zhang JT . Nootropic mechanisms of ginsenoside Rg1 — influence on neuronal plasticity and neurogenesis[J]. Yao Xue Xue Bao, 2005,40(5):385-388
doi: 10.3321/j.issn:0513-4870.2005.05.001
张均田 . 人参皂苷Rg1的促智作用机制——对神经可塑性和神经发生的影响[J]. 药学学报, 2005,40(5):385-388
doi: 10.3321/j.issn:0513-4870.2005.05.001
[1] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[2] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[3] Umesh Chandra Dash, Atish Kumar Sahoo. In vitro antioxidant assessment and a rapid HPTLC bioautographic method for the detection of anticholinesterase inhibitory activity of Geophila repens. Journal of Integrative Medicine, 2017, 15(3): 231-241.
[4] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[5] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[6] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[8] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[9] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[12] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[13] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[14] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
[15] Dilpesh Jain​, Manish Kumar Bansal, Rahul Dalvi​, Aman Upganlawar​, Rahul Somani​. Protective effect of diosmin against diabetic neuropathy in experimental rats. Journal of Integrative Medicine, 2014, 12(1): 35-41.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[4] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[5] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[6] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[7] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[8] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[9] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[10] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035