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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (4): 413-417.doi: 10.3736/jcim20060419

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of glycyrrhizin on adriamycin nephropathy in rats

Li-na Wang, Li Yu, Lei Zhang, Zhi-hong Hao, Dan Zhao, You-xiang Zhang   

  1. Department of Pediatrics, First People’s Hospital, Guangzhou, Guangdong Province 510180, China
  • Online:2006-07-31 Published:2006-07-15

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Objective

To observe the effects of glycyrrhizin on laminin (LN) expression in kidney tissue and excretory quantity of urine protein of rats with adriamycin nephropathy, and to explore the protective effects of glycyrrhizin on glomerulosclerosis.

Methods

Eighteen SD rats were randomly divided into 3 groups: normal control group (n=6), untreated group (n=6) and glycyrrhizin-treated group (n=6). Adriamycin nephropathy was induced in rats in the last two groups by intravenous injection of adriamycin. The rats in the glycyrrhizin-treated group were fed glycyrrhizin for eight weeks, whereas the rats in the normal control group and untreated group were fed normal saline solution for eight weeks too. The levels of 24 h urine protein (Upr), serum creatinine (sCr), blood urea nitrogen (BUN) and serum cholesterol (Ch) of rats in each group were examined before treatment and after the treatment for four and eight weeks. The renal morphological changes were observed unders a microscope. The expression level of LN in renal tissue was detected by streptavidin-biotin peroxidase complex (SABC) method.

Results

The levels of 24 h Upr after the treatment for four and eight weeks in the glycyrrhizin-treated group were both significantly decreased as compared with those in the untreated group. The pathological morphological changes of renal tissue in the glycyrrhizin-treated group were remarkably alleviated, and the expression level of LN in renal tissue was also decreased in the glycyrrhizin-treated group as compared with those in the untreated group.

Conclusion

The glycyrrhizin exerts certain protective effects on adriamycin nephropathy in rats by reducing excretory quantity of urine protein, decreasing expression level of LN in renal tissue, improving renal function and lessening the severity degree of glomerulosclerosis so as to retard the development of glomerulosclerosis.

Key words: Glycyrrhizin, Adriamycin, Glomerulosclerosis, Laminin, Rats

CLC Number: 

  • R692.6

Table 1

24 h urine protein of rats in different groups （ヌ±S, mg/24 h）"

Group n 24 h urine protein
Pre-treatment Treatment for 4 weeks Treatment for 8 weeks
Normal control 6 16.23±2.97 16.73±3.79 16.84±2.84
Untreated 6 16.78±3.23 60.62±4.68** 88.59±12.29**
Glycyrrhizin-treated 6 16.15±2.71 46.41±8.28**△△ 54.45±4.94**△△

Table 21

Serum levels of Cr, BUN and Ch of rats in different groups （ヌ±S）"

Group n Cr (μmol/L) BUN (mmol/L) Ch (mmol/L)
Normal control 6 35.3±6.9 5.2±0.6 1.62±0.32
Untreated 6 98.1±9.3** 23.2±3.5** 14.76±3.12**
Glycyrrhizin-treated 6 72.6±6.0**△△ 12.0±2.0**△△ 5.50±1.07**△△

Figure 1

Histopathologic changes of renal tissue in rats in different groups (HE staining, ×400) A: Glycyrrhizin-treated group; B: Untreated group; C: Normal control group."

Figure 2

Expression of LN in renal tissue of rats in different groups (SABC method, ×400) A: Glycyrrhizin-treated group; B: Untreated group; C: Normal control group."

Table 3

Expression levels of LN in renal tissue of rats in different groups（Cases）"

Group n Expression level of LN
– + ++ +++
Normal control 6 1 4 1 0
Untreated 6 0 1 2 3
Glycyrrhizin-treated 6 0 2 3 1
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