健脾清化方对慢性肾功能衰竭高脂血症大鼠肾组织氧自由基和转化生长因子β1 mRNA表达的影响

doi:10.3736/jcim20060418

Abstract

Objective

To evaluate the effects of Jianpi Qinghua Recipe (JPQHR) on oxygen radicals and transforming growth factor β1 (TGFβ1) in renal tissue in a rat model of chronic renal failure with hyperlipidemia.

Methods

Chronic renal failure with hyperlipidemia was induced in rats in untreated group and JPQHR-treated group by 5/6 nephrectomy and high fat diet. Then the rats in these two groups were fed distilled water and JPQHR respectively for eight weeks. The rats in normal control group received no specific interventions. After eight weeks of treatment, the levels of 24 h urine protein (Upr), blood urea nitrogen (BUN) and serum creatinine (Cr), cholesterol (Ch), triglycerides (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) of rats in these three groups were examined. The activity of superoxide dismutase (SOD), contents of malondialdehyde (MDA) and non-esterified fatty acids (NEFA) and expression level of TGFβ1 mRNA in renal tissue of rats in each groups were also determined.

Results

The levels of 24 Upr, BUN and serum Cr, Ch, TG and LDL in the JPQHR-treated group were significantly lower than those in the untreated group. The contents of MDA and NEFA and the expression level of TGFβ1 mRNA in the JPQHR-treated group were also significantly lower than those in the untreated group, while the activity of SOD was significantly increased in the JPQHR-treated group as compared with that in the untreated group.

Conclusion

The results indicate that JPQHR can improve the renal function of rats with chronic renal failure and hyperlipidemia by regulating lipid metabolism, maintaining balance between prooxidants and antioxidants and reducing expression of TGFβ1 mRNA in renal tissue.

Key words: Jianpi Qinghua Recipe, Chronic kidney failure, Hyperlipidemia, Oxygen radicals, Transforming growth factor beta

CLC Number:

R692.5

Qiang Fu, Li-qun He, He-xin Cao. Effects of Jianpi Qinghua Recipe on oxygen radicals and transforming growth factor β1 in renal tissue in a rat model of chronic renal failure with hyperlipidemia. Journal of Chinese Integrative Medicine, 2006, 4(4): 408-412.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060418

http://www.jcimjournal.com/EN/Y2006/V4/I4/408

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References 6

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[2]	何永成, 廖履坦, 丁小强 , 等. 实验性高胆固醇血症对健康大鼠肾皮髓质脂质成分的影响[J]. 肾脏病与透析肾移植杂志, 2000,9(3):254-256 doi: 10.3969/j.issn.1006-298X.2000.03.015
[3]	竺艳娟, 傅辰生, 庄亚敏 , 等. 高胆固醇血症对自发性高血压大鼠肾脏的损害[J]. 上海医科大学学报, 2000,27(4):274-276 doi: 10.3969/j.issn.1672-8467.2000.04.012
[4]	刘宏伟 . 原发性肾小球疾病湿热病理的临床研究[J]. 中医杂志, 1996,37(11):688-689
[5]	鲁明源 . 湿热体质与冠心病——冠心病危险因素的中医评析[J]. 山东中医药大学学报, 2003,27(1):16-20 doi: 10.3969/j.issn.1007-659X.2003.01.005
[6]	兰洋, 周钦, 吴兆龙 . Ox-LDL诱导大鼠系膜细胞TGF-beta1转录增强和NF-κB的活化[J]. 中华医学实践杂志, 2003,2(5):385-389

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Group	n	BUN (mmol/L)	sCr (μmol/L)	24 h Upr (mg/24 h)
Normal control	8	9.26±0.37	35.38±5.07	23.26±4.44
Untreated	7	32.32±10.80**	132.83±62.37**	63.17±14.66**
JPQHR-treated	6	18.59±7.19^△△	72.88±26.63^△△	38.77±9.46^△△

Group	n	Ch	TG	HDL	LDL
Normal control	8	1.56±0.25	0.40±0.07	0.96±0.19	0.35±0.07
Untreated	7	3.56±1.81**	1.65±1.48**	2.03±0.72**	0.86±0.50**
JPQHR-treated	6	2.55±0.40^△△	0.89±0.17^△△	1.64±0.24	0.54±0.09^△△

Group	n	SOD (μU/L)	MDA (pmol/L)	NEFA (mmol/L)
Normal control	8	409.94±50.28	12.90±3.11	19.95±15.64
Untreated	7	68.08±46.60**	27.66±1.84**	162.33±47.39**
JPQHR-treated	6	216.43±181.25^△△	21.68±3.40^△△	38.65±17.10^△△

Group	n	TGFβ1 mRNA
Normal control	8	0.24±0.03
Untreated	7	1.04±0.41**
JPQHR-treated	6	0.36±0.22^△△

Effects of Jianpi Qinghua Recipe on oxygen radicals and transforming growth factor β1 in renal tissue in a rat model of chronic renal failure with hyperlipidemia

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