甘草甜素对大鼠阿霉素肾病的保护作用

doi:10.3736/jcim20060419

Abstract

Objective

To observe the effects of glycyrrhizin on laminin (LN) expression in kidney tissue and excretory quantity of urine protein of rats with adriamycin nephropathy, and to explore the protective effects of glycyrrhizin on glomerulosclerosis.

Methods

Eighteen SD rats were randomly divided into 3 groups: normal control group (n=6), untreated group (n=6) and glycyrrhizin-treated group (n=6). Adriamycin nephropathy was induced in rats in the last two groups by intravenous injection of adriamycin. The rats in the glycyrrhizin-treated group were fed glycyrrhizin for eight weeks, whereas the rats in the normal control group and untreated group were fed normal saline solution for eight weeks too. The levels of 24 h urine protein (Upr), serum creatinine (sCr), blood urea nitrogen (BUN) and serum cholesterol (Ch) of rats in each group were examined before treatment and after the treatment for four and eight weeks. The renal morphological changes were observed unders a microscope. The expression level of LN in renal tissue was detected by streptavidin-biotin peroxidase complex (SABC) method.

Results

The levels of 24 h Upr after the treatment for four and eight weeks in the glycyrrhizin-treated group were both significantly decreased as compared with those in the untreated group. The pathological morphological changes of renal tissue in the glycyrrhizin-treated group were remarkably alleviated, and the expression level of LN in renal tissue was also decreased in the glycyrrhizin-treated group as compared with those in the untreated group.

Conclusion

The glycyrrhizin exerts certain protective effects on adriamycin nephropathy in rats by reducing excretory quantity of urine protein, decreasing expression level of LN in renal tissue, improving renal function and lessening the severity degree of glomerulosclerosis so as to retard the development of glomerulosclerosis.

Key words: Glycyrrhizin, Adriamycin, Glomerulosclerosis, Laminin, Rats

CLC Number:

R692.6

Li-na Wang, Li Yu, Lei Zhang, Zhi-hong Hao, Dan Zhao, You-xiang Zhang. Protective effects of glycyrrhizin on adriamycin nephropathy in rats. Journal of Chinese Integrative Medicine, 2006, 4(4): 413-417.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060419

http://www.jcimjournal.com/EN/Y2006/V4/I4/413

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Group	n	24 h urine protein
Group	n	Pre-treatment	Treatment for 4 weeks	Treatment for 8 weeks
Normal control	6	16.23±2.97	16.73±3.79	16.84±2.84
Untreated	6	16.78±3.23	60.62±4.68**	88.59±12.29**
Glycyrrhizin-treated	6	16.15±2.71	46.41±8.28**^△△	54.45±4.94**^△△

Group	n	Cr (μmol/L)	BUN (mmol/L)	Ch (mmol/L)
Normal control	6	35.3±6.9	5.2±0.6	1.62±0.32
Untreated	6	98.1±9.3**	23.2±3.5**	14.76±3.12**
Glycyrrhizin-treated	6	72.6±6.0**^△△	12.0±2.0**^△△	5.50±1.07**^△△

Group	n	Expression level of LN
Group	n	–	+	++	+++
Normal control	6	1	4	1	0
Untreated	6	0	1	2	3
Glycyrrhizin-treated	6	0	2	3	1

Protective effects of glycyrrhizin on adriamycin nephropathy in rats

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