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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (6): 606-610.doi: 10.3736/jcim20060613

• Original Experimental Research • Previous Articles     Next Articles

Study of Huoxue Qianyang Granules in revising the left ventricular hypertrophy of spontaneous hypertension rats

Duan Zhou1,Mei-fang Xiao2,Rong Hu1   

  1. 1. Department of Cardiovascular Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Department of Traditional Chinese Internal Medicine, Central Hospital of Huangpu District, Shanghai 200002, China
  • Online:2006-11-30 Published:2006-11-15

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Objective

To study the mechanism of Huoxue Qianyang Granule (HXQYG), a traditional Chinese compound medicine, in revising the left ventricular hypertrophy of hypertension.

Methods

Spontaneous hypertension rats (SHR) were randomly divided into seven groups: untreated group, Songling Xuemaikang (SLXMK)-treated group, captopril-treated group, high-, medium- and low-dose HXQYG-treated groups, and normal control group. The systolic blood pressure (SBP) and left ventricular mass index (LVMI) were measured. The content of angiotensin Ⅱ (AngⅡ) in left ventricular tissue was determined by radioimmunoassay. The expressions of angiotensin converting enzyme (ACE) protein and mRNA in left ventricular tissue were analyzed separately by immunohistochemical method and RT-PCR.

Results

(1) SBP and LVMI were higher in the untreated group than those in the normal control group, and they were lower in the high- and medium-dose HXQYG-treated groups than those in the untreated group, but higher than those in the captopril-treated group, and without significant difference as compared to those in the SLXMK-treated group. (2) The content of AngⅡ and expressions of ACE protein and mRNA in the left ventricular tissue in the untreated group were higher than those in the normal control group, and they were lower in the HXQYG-treated groups than those in the untreated group, but higher than those in the captopril-treated group, and without significant difference as compared to those in the SLXMK-treated group.

Conclusion

HXQYG can reverse the left ventricular hypertrophy of SHR, which may be due to decreasing the amount of AngⅡ and expressions of ACE protein and mRNA in the left ventricular tissue.

Key words: Hypertension, Left ventricular hypertrophy, Angiotensin Ⅱ, Angiotensin converting enzyme, Rats

CLC Number: 

  • R322.1+1

Table 1

Results of SBP of rats in 7 groups （ヌ±S, mm Hg）"

Group n The first SBP The last SBP
Normal control 9 133±2* 133±2*
Untreated 9 193±5 214±4
Captopril-treated 9 194±7 168±4**
SLXMK-treated 9 194±9 201±4*△
High-dose HXQYG-treated 9 193±5 193±6*△
Medium-dose HXQYG-treated 9 194±7 201±3*△
Low-dose HXQYG-treated 9 194±6 207±4*△

Table 2

Results of LVMI of rats in 7 groups（ヌ±S, mg/kg）"

Group n LVMI
Normal control 12 2.14±0.22**
Untreated 12 2.73±0.11
Captopril-treated 13 2.45±0.11**
SLXMK-treated 13 2.62±0.15*△△
High-dose HXQY-treated 10 2.58±0.05*△△
Medium-dose HXQY-treated 11 2.58±0.11*△△
Low-dose HXQY-treated 10 2.65±0.08△△

Table 3

Content of AngⅡ and expressions of ACE protein and mRNA in left ventricle of rats in 7 groups（ヌ±S）"

Group Content of AngⅡ
（n=11, pg/100 mg）		 ACE protein integral optical
density （n=7）		 ACE mRNA/β-actin
（n=7）
Normal control 1343±62* 13.61±2.28**△△ 0.39±0.18*
Untreated 1408±41 26.88±2.99 0.86±0.30
Captopril-treated 1316±45** 15.41±4.10** 0.57±0.13*
SLXMK-treated 1349±97 20.67±5.16**△△ 0.59±0.22*
High-dose HXQY-treated 1341±47* 18.17±1.46**△ 0.59±0.17*
Medium-dose HXQY-treated 1379±39△ 19.94±3.32**△ 0.57±0.27*
Low-dose HXQY-treated 1376±59 23.28±2.39△△ 0.73±0.37

Figure 1

Expression of ACE protein in left ventricle of rats (SABC, ×200) ACE mostly expressed in vascular endothelia cells and myocardial matrix cells, few in plasma and membrane of myocardial cells. The nigger-brown granules are indication of ACE (Red arrowhead). A: Normal control group, few expression of ACE; B: Untreated group, high expression of ACE; C: captopril-treated group, low expression of ACE ; D: High-dose HXQYG-treated group."

Figure 2

Electrophoretogram of ACE cDNAM: Marker; A: High-dose HXQYG-treated group; B: Medium-dose HXQYG-treated group; C: Low-dose HXQYG-treated group; D: Captopril-treated group; E: SLXMK-treated group; F: Untreated group; W: Normal control group. ACE: 317 bp; β-actin: 169 bp."

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