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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (2): 165-169.doi: 10.3736/jcim20070213

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbs for replenishing qi and resolving stagnation on hypoxia-inducible factor-1α and vascular endothelial growth factor in granulation tissue of skin ulcers in rats with diabetes

Hua-fa Que, Yuan-yin Zhu, Yun-fei Wang, Zhen Zhang, Jie-nan Xu, Jie Xing, Han-jun Tang   

  1. Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Online:2007-03-31 Published:2007-03-15

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Objective: To explore the effects of Chinese herbs for replenishing qi and resolving stagnation on hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in granulation tissue of skin ulcers in rats with syndrome of blood stasis and qi deficiency. 

Methods: Diabetic rats with back full-thickness skin lesion and syndrome of blood stasis and qi deficiency were divided in to five groups: untreated group, basic fibroblast growth factor (bFGF)-treated group, Yiqi Huayu Recipe (a recipe for replenishing qi and resolving stagnation)-treated group, Yiqi Recipe (a recipe for replenishing qi)-treated group and Huayu Recipe (a recipe for resolving stagnation)-treated group, and another eight normal rats served as normal control group. Immunohistochemical method and image analysis were used to test the expressions of HIF-1α and VEGF in granulation tissue of skin ulcers in rats with diabetes.

Results: In the untreated group, the expression of HIF-1α was significantly increased and the expression of VEGF was significantly decreased as compared with those in the normal control group (P＜0.01). The expression of HIF-1α was obviously lower (P＜0.01) and the expression of VEGF was significantly higher in the four drug treated groups as compared with those in the untreated group. In the Yiqi Huayu Recipe-treated group, the expression of HIF-1α was obviously lower than those in the bFGF-treated, Yiqi Recipe-treated and Huayu Recipe-treated groups (P＜0.05 or P＜0.01), and the expression of VEGF was significantly higher than that in the bFGF-treated group (P＜0.01). 

Conclusion: Chinese herbs for replenishing qi and resolving stagnation can promote the wound healing in rats through reducing the expression of HIF-1α, accelerating the expression of VEGF in granulation tissue of skin ulcers in rats with diabetes and ameliorating the status of ischemia and hypoxia.

Key words: diabetes mellitus, skin ulcers, hypoxia inducible factor 1α, vascular endothelial growth factor, rats

CLC Number: 

  • R285

Figure 1

Expression of HIF-1α in granulation tissue on skin ulcer wound in rats (immunohistochemical S-P method, ×100)A: Normal control group; B: Untreated group; C: bFGF-treated group; D: Yiqi Huayu Recipe-treated group; E: Yiqi Recipe-treated group; F: Huayu Recipe-treated group."

Figure 2

Expression of VEGF in granulation tissue on skin ulcer wound in rats (immunohistochemical S-P method, ×100)A: Normal control group; B: Untreated group; C: bFGF-treated group; D: Yiqi Huayu Recipe-treated group; E: Yiqi Recipe-treated group; F: Huayu Recipe-treated group."

Table 1

Expressions of HIF-1α and VEGF in granulation tissue on skin ulcer wound in rats（μm2, $\bar{x}±S$）"

GroupnHIF-1αVEGF
Normal control811 682.58±645.2818 282.97±1 886.87
Untreated819 373.04±913.40**11 164.89±654.88**
bFGF-treated812 976.79±926.35▲▲ 12 869.68±989.89**▲▲
Yiqi Huayu Recipe-treated811 713.43±900.69△▲▲ 15 823.42±1 822.20**△△▲▲
Yiqi Recipe-treated813 364.61±1 290.13**▲▲□□ 13 057.01±645.94**▲▲
Huayu Recipe-treated813 948.22±1 774.90**▲▲□□ 12 123.79±833.63**
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