益气化瘀中药对糖尿病皮肤溃疡大鼠缺氧诱导因子-1α和血管内皮细胞生长因子的影响

doi:10.3736/jcim20070213

Abstract

Objective: To explore the effects of Chinese herbs for replenishing qi and resolving stagnation on hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in granulation tissue of skin ulcers in rats with syndrome of blood stasis and qi deficiency.

Methods: Diabetic rats with back full-thickness skin lesion and syndrome of blood stasis and qi deficiency were divided in to five groups: untreated group, basic fibroblast growth factor (bFGF)-treated group, Yiqi Huayu Recipe (a recipe for replenishing qi and resolving stagnation)-treated group, Yiqi Recipe (a recipe for replenishing qi)-treated group and Huayu Recipe (a recipe for resolving stagnation)-treated group, and another eight normal rats served as normal control group. Immunohistochemical method and image analysis were used to test the expressions of HIF-1α and VEGF in granulation tissue of skin ulcers in rats with diabetes.

Results: In the untreated group, the expression of HIF-1α was significantly increased and the expression of VEGF was significantly decreased as compared with those in the normal control group (P＜0.01). The expression of HIF-1α was obviously lower (P＜0.01) and the expression of VEGF was significantly higher in the four drug treated groups as compared with those in the untreated group. In the Yiqi Huayu Recipe-treated group, the expression of HIF-1α was obviously lower than those in the bFGF-treated, Yiqi Recipe-treated and Huayu Recipe-treated groups (P＜0.05 or P＜0.01), and the expression of VEGF was significantly higher than that in the bFGF-treated group (P＜0.01).

Conclusion: Chinese herbs for replenishing qi and resolving stagnation can promote the wound healing in rats through reducing the expression of HIF-1α, accelerating the expression of VEGF in granulation tissue of skin ulcers in rats with diabetes and ameliorating the status of ischemia and hypoxia.

Key words: diabetes mellitus, skin ulcers, hypoxia inducible factor 1α, vascular endothelial growth factor, rats

CLC Number:

R285

Hua-fa Que, Yuan-yin Zhu, Yun-fei Wang, Zhen Zhang, Jie-nan Xu, Jie Xing, Han-jun Tang. Effects of Chinese herbs for replenishing qi and resolving stagnation on hypoxia-inducible factor-1α and vascular endothelial growth factor in granulation tissue of skin ulcers in rats with diabetes. Journal of Chinese Integrative Medicine, 2007, 5(2): 165-169.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20070213

http://www.jcimjournal.com/EN/Y2007/V5/I2/165

TrendMD

Figures/Tables 3

Figure 1

Figure 2

Table 1

References 13

[1]	阙华发, 唐汉钧, 向寰宇, 等.益气化瘀法治疗糖尿病皮肤溃疡38例临床观察[J].中西医结合学报,2004,2(1):63-64
	Que HF, Tang HJ, Xiang HY, et al.Treatment of diabetic skin ulcers by invigorating Qi and dispersing stagnation: A report of 38 cases[J].Zhong Xi Yi Jie He Xue Bao,2004,2(1):63-64
[2]	陈小野. 实用中医证候动物模型学[M].北京: 北京医科大学、中国协和医科大学联合出版社,1993.233-235
	Chen XY.Practical animal model of TCM syndrome[M].Beijing: Joint Publishing House of Peking Medical College and Peking Union Medical College,1993.233-235
[3]	赵辉, 王键, 李净, 等.多因素复合制作气虚血瘀证脑缺血的动物模型体会[J].河南中医,2001,21(4):18-20
	Zhao H, Wang J, Li J, et al.Establishment of an animal model of qi-deficiency and blood stasis syndrome in rats with cerebral ischemia[J].Henan Zhong Yi,2001,21(4):18-20
[4]	付小兵, 王德文.现代创伤修复学[M].北京: 人民军医出版社,1999.229-230
	Fu XB, Wang DW.Repair of injury in modern time[M].Beijing: People's Military Medical Press,1999.229-230
[5]	青春, 林炜栋, 刘琰, 等.糖尿病合并创面难愈等并发症形成机制的研究. //陆树良. 烧伤创面愈合机制与新技术(第2版)[M].北京: 人民军医出版社,2003,(第2版):135-154
	Qing C, Lin WD, Liu Y, et al.Study of the formation mechanism of diabetic complications such as wound repair. In: LU SL. Mechanism and advanced technology of the wound repair in full-thickness burns(2nd ed)[M].Beijing: People's Military Medical Press,2003,(2nd ed):135-154
[6]	王争, 张纪蔚, 张柏根.糖尿病足动脉缺血患者骨骼肌血管内皮生长因子基因表达[J].中华外科杂志,2002,40(7):505-507
	Wang Z, Zhang JW, Zhang BG.Vascular endothelial growth factor gene expression in patients' ischemic skeletal muscle with diabetic foot[J].Zhonghua Wai Ke Za Zhi,2002,40(7):505-507
[7]	朱元颖, 阙华发.缺氧诱导因子1与祛瘀生新[J].中西医结合学报,2006,4(1):94-97
	Zhu YY, Que HF.Hypoxia-inducible factor-1 and its correlation with removing blood stasis for promoting tissue regeneration of traditional Chinese principle of treatment[J].Zhong Xi Yi Jie He Xue Bao,2006,4(1):94-97
[8]	Pugh CW, Ratcliffe PJ.Regulation of angiogenesis by hypoxia: role of the HIF system[J].Nat Med,2003,9(6):677-684 doi: 10.1038/nm0603-677 pmid: 12778166
[9]	Marti HJ, Bernaudin M, Bellail A, et al.Hypoxia-induced vascular endothelial growth factor expression precedes neovascularization after cerebral ischemia.[J].Am J Pathol,2000,156(3):965-976
[10]	Cramer T, Schipani E, Johnson RS, et al.Expression of VEGF isoforms by epiphyseal chondrocytes during low-oxygen tension is HIF-1 alpha dependent[J].Osteoarthritis Cartilage,2004,12(6):433-439
[11]	Abraham D, Krenn K, Seebacher G, et al.Upregulated hypoxia-inducible factor-1 DNA binding activity to the vascular endothelial growth factor-A promoter mediates increased vascular permeability in donor lung grafts[J].Ann Thorac Surg,2004,77(5):1751-1755 doi: 10.1016/j.athoracsur.2003.10.050
[12]	Semenza GL, Agani F, Feldser D, et al.Hypoxia, HIF-1,the pathophysiology of common human diseases[J].Adv Exp Med Biot,2000,475():123-130
[13]	Semenza GL.O2-regulated gene expression: transcriptional control of cardiorespiratory physiology by HIF-1[J].J Appl Physiol,2004,96(3):1173-1177; discussion 1170-1172

Related Articles 15

[1]	Yunita Sari, Akhyarul Anam, Annas Sumeru, Eman Sutrisna. The knowledge, attitude, practice and predictors of complementary and alternative medicine use among type 2 diabetes mellitus patients in Indonesia. Journal of Integrative Medicine, 2021, 19(4): 347-353.
[2]	Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[3]	Pukar Khanal, Basanagouda M. Patil. Integration of network and experimental pharmacology to decipher the antidiabetic action of Duranta repens L.. Journal of Integrative Medicine, 2021, 19(1): 66-77.
[4]	Baoyi Shao, Saiying Hou, Yuenyan Chan, Changchun Shao, Lixing Lao. Remission of new-onset type 2 diabetes mellitus in an adolescent using an integrative medicine approach: A case report. Journal of Integrative Medicine, 2021, 19(1): 85-88.
[5]	Abdulhakim Abubakar, Abdullahi Balarabe Nazifi, Idris Mohammed Maje, Yusuf Tanko, Joseph Akpojo Anuka, Ezzeldin Mukthar Abdurahman. Antihyperglycaemic activity of ethylacetate extract of Chlorophytum alismifolium in type 2 diabetes: The involvement of peroxisome proliferator-activated receptor-γ and dipeptidyl peptidase-4. Journal of Integrative Medicine, 2021, 19(1): 78-84.
[6]	Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[7]	Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[8]	Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[9]	Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[10]	Olakunle Bamikole Afolabi, Omotade Ibidun Oloyede, Shadrack Oludare Agunbiade. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe²⁺-induced pancreatic oxidative stress. Journal of Integrative Medicine, 2018, 16(3): 192-198.
[11]	Chun-song Hu, Tengiz Tkebuchava. SEEDi^1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[12]	Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[13]	Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[14]	Ausanee Wanchai, Duangjai Phrompayak. Use of complementary and alternative medicine among Thai patients with type 2 diabetes mellitus. Journal of Integrative Medicine, 2016, 14(4): 297-305.
[15]	Yee Chi Peggy Wong. Need of integrated dietary therapy for persons with diabetes mellitus and “unhealthy” body constitution presentations. Journal of Integrative Medicine, 2016, 14(4): 255-268.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2]	Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3]	Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4]	SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5]	Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6]	Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7]	Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8]	Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9]	Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10]	Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239

Group	n	HIF-1α	VEGF
Normal control	8	11 682.58±645.28	18 282.97±1 886.87
Untreated	8	19 373.04±913.40**	11 164.89±654.88**
bFGF-treated	8	12 976.79±926.35^▲▲	12 869.68±989.89**^▲▲
Yiqi Huayu Recipe-treated	8	11 713.43±900.69^△▲▲	15 823.42±1 822.20**^△△▲▲
Yiqi Recipe-treated	8	13 364.61±1 290.13**^▲▲□□	13 057.01±645.94**^▲▲
Huayu Recipe-treated	8	13 948.22±1 774.90**^▲▲□□	12 123.79±833.63**

Effects of Chinese herbs for replenishing qi and resolving stagnation on hypoxia-inducible factor-1α and vascular endothelial growth factor in granulation tissue of skin ulcers in rats with diabetes

RichHTML

PDF (PC)

Like