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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 531-535.doi: 10.3736/jcim20070511

• Original Experimental Research • Previous Articles     Next Articles

Effects of Jianpi Bushen Huoxue Recipe on hematopoietic cell apoptosis in a mouse model of aplastic anemia

Ming-hui Hu1, Yong-ming Zhou1, Wen-wei Zhu1, Zhi-zhong Xue2, Jia-hui Lu1   

  1. 1. Department of Hematology , Yueyang Hospital of Integrated Traditional Chinese and Western Medicine , Shanghai University of Traditional Chinese Medicine , Shanghai 200437 , China
    2. Department of Clinical Laboratory , Yueyang Hospital of Integrated Traditional Chinese and Western Medicine , Shanghai University of Traditional Chinese Medicine , Shanghai 200437 , China
  • Online:2007-09-20 Published:2007-09-15

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Objective: Adopting methods of cell culture to explore the effects and mechanisms of Jianpi Bushen Huoxue Prescription (JPBSHXP), a traditional Chinese compound herbal medicine for strengthening spleen, reinforcing kidney and activating blood circulation, in inhibiting hematopoietic cells apoptosis in a mouse model of aplastic anemia (AA).Methods: Blood serum of AA mice was made from an AA mouse model. Blood serums containing different traditional Chinese compound herbal medicine were made from rats after intragastric administration of JPBSHXP and its related decoctions, respectively. Bone marrow cells of normal mice were incubated by these blood serums for 24 hours, respectively. The apoptosis of the bone marrow cells were assayed by flow cytometry and transmission electron microscopy (TEM).Results: It was indicated that the bone marrow cells of normal mice incubated with blood serum of AA mice displayed typical apoptosis. The apoptosis rates of bone marrow cells of the AA mice incubated by blood serum containing different traditional Chinese herbal medicine were decreased. The effect of Bushen (reinforcing kidey) Recipe was better than Jianpi (strengthening spleen) Recipe and Huoxue (activating blood circulation) Recipe, while the effect of JPBSHXP was the best. TEM results showed that the effect of Bushen Recipe was better than that of the Jianpi Recipe and the Huoxue Recipe, while the effect of JPBSHXP was the best.Conclusion: JPBSHXP and its related decoctions can significantly decrease the apoptosis rate of bone marrow mononuclear cells of the AA mice. It is inferred that JPBSHXP can promote bone marrow hematogenesis.

Key words: anemia, aplastic, strengthening spleen, reinforcing kidney, activating blood circulation, hemato poiesis , medullary, apoptosis, mice

CLC Number: 

  • R556.5

Table 1

Changes of hemogram in normal control and untreated group (ヌ±S)"

Group n White blood cell (×109/L) Hemoglobin (g/L) Platelet (×109/L)
Normal control 10 7.29±0.53 141.60±12.90 496.00±30.39
Untreated 10 1.31±0.60** 97.20±15.62** 104.80±30.14**

Figure 1

Pathological characteristics of bone marrow in normal control group and untreated group (HE staining, ×40) A: Normal control group; B: Untreated group."

Table 2

Effects of blood serums containing different traditional Chinese compound herbal medicines on apoptosis rates of bone marrow cells in different groups（ヌ±S, %）"

Group n Apoptosis rate
Normal control 7 8.6±4.1
Blank control 7 13.0±5.7
Untreated 9 39.9±12.7**
Jianpi Recipe-treated 7 23.4±5.0△△▲▲
Bushen Recipe-treated 7 13.7±2.1△△▲
Huoxue Recipe-treated 7 21.8±5.6△△▲▲
Jianpi Bushen Recipe-treated 5 18.9±1.7△△▲▲
Whole prescription-treated 7 10.3±3.2△△

Figure 2

Characteristics of bone marrow cells in blood serum in different groups (transmission electron microscopy, ×7 000)A: Normal control group; B: Blank control group; C: Untreated group; D: Jianpi Recipe-treated group; E: Bushen Recipe-treated group; F: Huoxue Recipe-treated group; G: Jianpi Bushen Recipe-treated group; H: Whole prescription-treated group."

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