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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 536-540.doi: 10.3736/jcim20070512

• Original Experimental Research • Previous Articles     Next Articles

Effects of Astragalus on expression of renal angiopoietin receptor Tie-2 in diabetic rats

Hong-wei Gu1, Zhao-hui Ni1, Le-yi Gu1, Yu-cheng Yan1, Hui-li Dai1, Ning-li Li2, Min-fang Zhang1, Jia-qi Qiang1   

  1. 1. Department of Nephrology，Renji Hospital，Medical College of Shanghai Jiaotong University，Shanghai 200127，China
    2. Institute of Immunology，Medical College of Shanghai Jiaotong University，Shanghai 200025，China
  • Online:2007-09-20 Published:2007-09-15

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Objective: To study the expression of angiopoietin receptor Tie-2 in the renal tissue of diabetic rats and the effects of Astragalus.Methods: SD rats were randomly divided into normal control group, diabetes group and Astragalus-treated group. The expression of receptor Tie-2 in the renal tissue was assessed by using real-time quantitative polymerase chain reaction and immunohistochemical method.Results: Glomerule Tie-2 protein expression was significantly elevated in the diabetes group as compared with the normal control group (P＜0.01). Glomerule Tie-2 protein expression in the Astragalus-treated group was decreased as compared with the diabetes group (P＜0.01).Conclusion: Tie-2 may play an important role in the pathogenesis of the early stage diabetic renal injury. The reno-protection effect of Astragalus may be mediated by down-regulating the expression of Tie-2 in the kidney tissue of diabetic rats.

Key words: diabetic nephropathies, angiogenesis factor, Astragalus, rats

CLC Number: 

  • R587.24

Table 1

Sequences of primers for real-time PCR amplified genes"

mRNA Sequence Forward primer Reverse primer Length
β-actin 419-499 5'-AGGCCAACCGTGAAAAGATG-3' 5'-ACCAGAGGCATACAGGGACAA-3' 81 bp
Tie-2 2181-2252 5'-TGCCACCATCACTCAATACCA-3' 5'-AGGCTGGGTTGCTTGATCCT-3' 72 bp

Table 2

Metabolic and physiological parameters in three groups (ヌ±S)"

Group n BG （mmol/L） BW (g) KWI (mg/g) SBP (mmHg) Upro/Cr （μg/μmol）
Normal control 6 12.68±1.14 467.64±6.32 0.30±0.05 114.74±8.24 61.58±7.51
Diabetes 6 37.07±10.67** 254.50±60.45** 0.59±0.07** 139.50±6.08** 169.21±19.19**
Astragalus-treated 6 45.56±10.15** 221.67±67.78** 0.68±0.13** 143.17±17.31** 117.90±44.85**△△

Table 3

Comparisons of VG and RM among three groups (ヌ±S)"

Group n VG(×106, μm3) RM(%)
Normal control 6 18.77±3.89 37.78±3.97
Diabetes 6 31.47±8.00** 52.30±9.44**
Astragalus-treated 6 29.37±3.62** 54.62±8.13**

Table 4

Expression of renal angiopoietin receptor Tie-2 in three groups (ヌ±S)"

Group n Tie-2 mRNA Tie-2 protein (%)
Normal control 6 132.13±63.12 26.55±3.94
Diabetes 6 156.31±33.34 43.04±9.45**
Astragalus-treated 6 117.72±47.92 28.02±6.47△△

Figure 1

Renal Tie-2 expression in 3 groups (Immunohistochemical method, ×400) A: Normal control group; B: Diabetes group; C: Astragalus-treated group."

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