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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 524-529.doi: 10.3736/jcim20080518

• Original Experimental Research • Previous Articles     Next Articles

Volatile oil of Magnolia biondii inhibits expressions of P-selectin protein in serum and renal tissue of rats with diabetic nephropathy

Xue-peng Sun, Nian-song Wang(), Qin Xue, Feng Wang   

  1. Department of Nephrology, the 6th Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China
  • Received:2008-03-19 Online:2008-05-20 Published:2008-05-15
  • Contact: WANG Nian-song E-mail:wangniansong@yahoo.com.cn

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Objective: To investigate the protection mechanism of volatile oil of Magnolia biondii Pamp. (VOMBP) against diabetic nephropathy in rats by observing its effects on level of soluble P-selectin (sP-selectin) in serum and expression of P-selectin in renal tissue.
Methods: Fifty SD rats were randomly divided into normal control group, untreated group, and low-, medium- and high-dose VOMBP-treated group. Diabetic nephropathy was induced in rats by intraperitoneal injection of 1% streptozotocin. Before and the 1st day, 4th, 8th and 12th week after the induction, random blood glucose (RBG) and 24-hour urinary micro-albumin were detected in different groups. At the 12th week, the rats were sacrificed to collect the blood samples and renal tissues. The contents of blood urea nitrogen (BUN) and serum creatinine (SCr) were detected and the pathological change in renal tissues was observed by light microscope; the level of sP-selectin was detected by enzyme-linked immunosorbent assay and the expression of P-selectin protein in renal tissues was measured by immunohistochemical method.
Results: Compared with the normal control group, RBG, 24-hour urinary micro-albumin, the contents of BUN, sP-selectin in serum and expression of P-selectin protein in renal tissue in the untreated group were significantly increased (P<0.01), the content of SCr was significantly decreased (P<0.01), and pathological change of renal tissues was also obvious. Compared with the untreated group, 24-hour urinary micro-albumin, the level of sP-selectin in serum and expression P-selectin protein in renal tissue of the three VOMBP-treated groups were all significantly decreased (P<0.01), and pathological change was lessened too. However, there were no significant differences among the three VOMBP-treated groups (P>0.05).
Conclusion: VOMBP can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue.

Key words: diabetic nephropathy, P-selectin, Magnolia biondii Pamp, oils, volatile, rats, diabetes mellitus

CLC Number: 

  • R587.1

Table 1

Random blood glucose of rats in different groups （$\bar{x}$±s, mmol/L）"

Group n Before model
establishing		 After model established
1st day 4th week 8th week 12th week
Normal control 10 6.80±0.48 6.70±0.36 6.14±0.68 6.38±0.43 5.60±0.28
Untreated 10 7.03±0.54 22.64±1.71** 31.15±0.54** 29.41±4.6** 32.00±0.12**
Low-dose VOMBP-treated 10 6.38±0.68 24.47±2.09** 30.23±3.39 ** 30.83±3.21** 30.67±1.86**
Medium-dose VOMBP -treated 10 6.48±0.49 23.15±1.62** 30.75±2.90** 29.67±4.71** 27.08±5.70**
High-dose VOMBP -treated 10 6.72±0.41 25.00±2.33** 30.96±2.70** 29.46±2.79** 29.85±2.97**

Table 2

24-hour urinary micro-albumin of rats in different groups （$\bar{x}$±s, mg/24 h）"

Group n Before model
establishing		 After model established
1st day 4th week 8th week 12th week
Normal control 10 0.065±0.034 0.070±0.030 0.068±0.036 0.057±0.029 0.052±0.024
Untreated 10 0.067±0.042 0.238±0.048** 0.451±0.279** 0.664±0.424** 1.305±0.305**
Low-dose VOMBP-treated 10 0.096±0.013 0.275±0.049** 0.311±0.058** 0.599±0.286** 0.373±0.057**△△
Medium-dose VOMBP -treated 10 0.087±0.009 0.276±0.063** 0.463±0.324** 0.463±0.307** 0.461±0.254**△△
High-dose VOMBP -treated 10 0.081±0.010 0.274±0.049** 0.356±0.133** 0.590±0.299** 0.538±0.223**△△

Table 3

Contents of BUN and SCr in rats in different groups at the 12th week （$\bar{x}$±s）"

Group n BUN (mmol/L) SCr (μmol/L)
Normal control 10 6.31±0.98 40.07±7.11
Untreated 10 12.29±4.83** 27.11±7.69**
Low-dose VOMBP-treated 10 10.17±2.17 ** 26.33±7.77 **
Medium-dose VOMBP-treated 10 9.96±1.01 ** 23.60±3.91**
High-dose VOMBP-treated 10 10.94±2.22** 27.50±6.48 **

Figure 1

Results of pathological section of kidney in rats in different groups (PAS staining, ×200） A: Normal control group; B: Untreated group; C: Low-dose VOMBP-treated group; D: Medium-dose VOMBP-treated group; E: High-dose VOMBP-treated group."

Table 4

Talbe 4 Content of sP-selectin in serum and expressin of P-selectin protein in renal tissue of rats in different groups at the 12th week （$\bar{x}$±s）"

Group n sP-selectin (ng/L) P-selectin protein expression
Normal control 10 46.41±10.04 0.104±0.026
Untreated 10 82.50±24.37 ** 0.214±0.030**
Low-dose VOMBP-treated 10 52.88±26.35▲▲ 0.117±0.017△△
Medium-dose VOMBP-treated 10 22.72±14.16**△△ 0.119±0.021△△
High-dose VOMBP-treated 10 46.29±25.98△△ 0.133±0.030△△

Figure 2

Expression of P-selectin protein in renal tissue of rats in different groups (Immunohistochemical staining, ×200） A: Normal control group; B: Untreated group; C: Low-dose VOMBP-treated group; D: Medium-dose VOMBP-treated group; E: High-dose VOMBP-treated group."

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