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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (6): 611-615.doi: 10.3736/jcim20080613

• Original Experimental Research • Previous Articles     Next Articles

Preventive and therapeutic effects of Yishen Huanji Decoction on kidney injury in rats induced by simulation of military overtraining

Hong Chen(), Jun Yang, Chun-hua Zhou   

  1. Department of Nephrology, Navy General Hospital of People's Liberation Army, Beijing 100037, China
  • Received:2007-11-05 Online:2008-06-20 Published:2008-06-15
  • Contact: CHEN Hong E-mail:chenhongqjhl@sina.com

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Objective: To observe the preventive and therapeutic effects of Yishen Huanji Decoction (YSHJD), a compound traditional Chinese herbal medicine, on military overtraining-induced kidney injury in a rat model.
Methods: Thirty SD rats were randomly divided into normal control group,untreated group and YSHJD-treated group. The military overtraining-induced kidney injury in rats was established by forcing to run on the treadmill for 8 weeks. The rats in YSHJD-treated group were administered with YSHJD at the same time. The 24-hour urines were collected every weekend for detecting the contents of urinary sediment, 24-hour urine total protein, 24-hour urine albumin and activity of N-acetyl-β-D-glucosaminidase (NAG). The blood and renal tissues were collected after 8-week training, and the levels of serum urea nitrogen (BUN) and creatinine (SCr) were detected. Angiotensin Ⅱ (AngⅡ) was detected by radioimmunoassay and activity of Na +, K +-ATPase in kidney was analyzed by chemical colorimetric method.
Results: Compared with the normal control group, after 8-week training, the contents of 24-hour urine protein, activities of NAG in urine, and the levels of BUN and SCr in rats in the untreated group and YSHJD-treated group were obviously increased (P<0.05), and these parameters of the YSHJD-treated group were lower than those of the untreated group (P<0.05). The contents of AngⅡ in blood plasma and kidney of rats in the untreated group and YSHJD-treated group were higher than those of the normal control group (P<0.05), and had no statistical difference between the untreated group and YSHJD-treated group. Compared with the normal control group, the activity of Na +, K +-ATPase in kidney of rats in the untreated group was obviously decreased (P<0.05), and had no statistical difference between the normal control group and YSHJD-treated group.
Conclusion: YSHJD can protect against military overtraining-induced kidney injury in rats by decreasing the contents of 24-hour urine protein, BUN and SCr, and the activity of NAG, and increasing the activity of Na +, K +-ATPase.

Key words: athletic injury, nephrosis, military medicine, Yishen Huanji Decoction, rats

CLC Number: 

  • R289.5

Table 1

Contents of total protein and albumin and activity of NAG in 24-hour urine of rats in three groups ($\bar{x}$±s)"

Group n Urinary total protein (mg) Urinary albumin (mg) NAG (U)
Normal control
Before exercise 10 0.81±0.12 0.13±0.05 0.49±0.08
2 weeks 10 1.09±0.11 0.19±0.05 0.59±0.08
3 weeks 10 1.38±0.17 0.27±0.05 0.62±0.11
8 weeks 10 4.15±0.46 0.78±0.10 0.83±0.10
Untreated
Before exercise 10 0.83±0.13 0.15±0.04 0.52±0.10
2 weeks 10 1.11±0.12 0.21±0.05 0.74±0.15*
3 weeks 10 1.79±0.18* 0.45±0.07* 0.95±0.12*
8 weeks 10 19.76±1.75* 3.79±0.14* 3.85±0.19*
YSHJD-treated
Before exercise 10 0.82±0.09 0.17±0.03 0.55±0.12
2 weeks 10 1.05±0.20 0.20±0.06 0.72±0.11*
3 weeks 10 1.83±0.14* 0.41±0.06* 0.91±0.12*
8 weeks 10 8.06±0.35*△ 1.61±0.11*△ 2.32±0.23*△

Table 2

Contents of BUN and SCr of rats in three groups at the end of 8-week exercise （$\bar{x}$±s）"

Group n BUN (mmol/L) SCr (μmol/L)
Normal control 10 6.33±0.84 38.39±4.60
Untreated 10 12.49±0.52* 77.78±5.01*
YSHJD-treated 10 7.57±0.77*△ 56.86±3.70*△

Table 3

Contents of AngⅡ in blood plasma and renal tissue, and activities of Na+, K+-ATPase in renal tissue of rats in three groups at the end of 8-week exercise （$\bar{x}$±s）"

Group n AngⅡ in blood plasma (ng/L) AngⅡ in renal tissue (ng/g) Na+, K+-ATPase (U/mg)
Normal control 10 118.33±11.71 20.71±0.51 4.16±0.27
Untreated 10 586.57±40.85* 60.44±1.03* 2.18±0.24*
YSHJD-treated 10 469.83±38.57* 59.78±2.17* 4.11±0.23△
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