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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (6): 606-610.doi: 10.3736/jcim20080612

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xinfeng Capsule on behaviors and myocardial ultrastructure in adjuvant arthritis rats

Jian Liu(), Xue-fang Yu, Rui-kai Zong   

  1. Department of Rheumatology, the First Affiliated Hospital, Anhui College of Traditional Chinese Medicine, Hefei, Anhui Province 230031,China
  • Received:2007-09-02 Online:2008-06-20 Published:2008-06-15
  • Contact: LIU Jian E-mail:liujianahzy@yahoo.com.cn

Objective: To observe the effects of Xinfeng Capsule (XFC), a compound Chinese herbal medicine, on behaviors and myocardial ultrastructure in adjuvant arthritis (AA) rats.
Methods: Fifty-five rats were randomly divided into normal control group, untreated group, methotrexate (MTX) group, Tripterygium wilfordii glycosides tablet (TGT) group and XFC group. There were 11 rats in each group. Except for those in the normal control group, the rats in the other groups were all intracutaneously given 0.1 ml Freund's complete adjuvant in the right hind limb. Changes of behaviors and myocardial ultrastructure of the rats in different groups were observed.
Results: Voix pedis' swelling and arthritis index in XFC, MTX and TPT groups were all obviously improved as compared with the untreated group. After XFC treatment, the autonomic activities were obviously increased, number of errors in exercise period and test period were obviously decreased, step-down latency (SDL) was lengthened and escape latency (EL) was shortened. Overall structure of myocardial myofibril and the majority of cristae was intact in XFC group. The effects of XFC in improving the behaviors and myocardial ultrastructure were better than those of MTX and TPT.
Conclusion: Changes of behaviour and myocardial ultrastructure of AA rats can be observed. XFC can improve the myocardial ultrastructure of AA rats as well as their behaviors.

Key words: Xinfeng Capsule, adjuvant arthritis, behavior, myocardium, rats

CLC Number: 

  • R737.9

Table 1

Changes of body weight in different groups ($\bar{x}$±s, g)"

Group n Body weight
At day one At day 19 At day 49
Normal control 11 217.27±27.87 262.27±12.52 327.27±59.64
Untreated 11 204.09.±23.86 217.27±40.27** 241.36±35.15**
MTX 11 211.36±22.92 224.09±31.61** 306.82±37.63
TGT 11 214.09±13.38 222.73±31.81** 311.64±65.33△△
XFC 11 221.82±20.53 232.27±12.32* 358.18±85.42△△▲□

Table 2

Changes of voix pedis' swelling and arthritis index in different groups ($\bar{x}$±s)"

Group n Voix pedis' swelling (ml) Arthritis index
At day 19 At day 49 At day 19 At day 49
Normal control 11 36.68±25.66 37.74±23.64 0.00±0.00 0.00±0.00
Untreated 11 71.80±21.24** 68.92±28.48** 7.27±1.01** 8.09±1.45**
MTX 11 59.27±15.48* 36.50±16.71△△ 6.82±0.75** 3.18±1.25**△△
TGT 11 69.43±25.87** 51.73±11.74*▲ 7.27±1.01** 5.36±2.46**△△▲▲
XFC 11 65.37±20.78** 44.21±22.29△△ 7.45±1.13** 3.18±1.33**△△□□

Table 3

Behavior changes in different groups ($\bar{x}$±s)"

Group n Autonomic Activity Step-down
latency (s)
Escape latency (s) Errors in
exercise period
Errors in test period
Normal control 11 60.36±12.44 80.73±15.68 10.73±2.97 3.64±1.29 1.45±0.69
Untreated 11 19.27±9.69** 41.27±9.18** 9.64±4.37** 9.55±1.63** 7.45±1.57**
MTX 11 52.45±12.30△△ 69.09±8.02*△△ 15.00±4.65△△* 4.09±1.14△△ 2.28±1.01△△
TGT 11 56.45±7.63△△ 74.18±7.45△△ 13.18±2.89△△ 3.46±1.04△△ 2.18±0.75△△
XFC 11 60.09±7.49△△ 79.00±11.80△△▲ 13.09±4.16△△ 3.91±1.51△△ 2.00±1.09△△

Figure 1

Ultrastructure of cardiac muscle cells in different groups (Transmission electron microscope, ×8 000) A: Normal control group; B: Untreated goup; C: MTX group; D: TGT group; E: XFC group."

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