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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (9): 960-963.doi: 10.3736/jcim20080917

• Original Experimental Research • Previous Articles     Next Articles

Effects of Quyu Jiedu Granule on expressions of tumor necrosis factor-α and interleukin-6 mRNAs in ovarian granulosa cells of endometriosis rats

Xin-ling Li1, Fang Lian2(), Yan-he Liu2   

  1. 1. Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province 250014, China
    2. Reproductive and Genetic Center of Integrative Medicine, the Affiliated Hospital, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province 250014, China
  • Received:2008-01-09 Online:2008-09-20 Published:2008-09-15

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Objective: To observe the effects of Quyu Jiedu Granule, a compound traditional Chinese herbal medicine for removing blood stasis and expelling superficial evils, on the quality of oocytes and the expressions of tumor necrosis factor-alpha (TNF-α)and interleukin-6 (IL-6) mRNAs in ovarian granulosa cells of endometriosis (EM) rats.
Methods: Forty EM rats were randomly divided into two groups: experimental group and control group. There were 20 EM rats in each group. The uteri of another 20 SD rats were drawn in sham-operated group. The number and percentage of high quality oocytes and the levels of TNF-α and IL-6 mRNA expressions in the granulosa cells of EM rats were detected by using reverse transcription-polymerase chain reaction.
Results: The number and percentage of high quality oocytes in the experimental group were significantly higher than those in the control group (P<0.05), and the levels of TNF-α and IL-6 mRNAs in granulose cells in the experimental group were significantly lower than those in the control group (P<0.05).
Conclusion: The increase of the TNF-α and IL-6 mRNA expressions in ovarian granulose cells of EM rats leads to the decrease of the oocyte quality. The mechanism of Quyu Jiedu Granule in improving the quality of oocytes may be related to the decrease of TNF-α and IL-6 mRNA expressions in ovarian granulosa cells.

Key words: endometriosis, Quyu Jiedu Granule, ovary, granulosa cells, tumor necrosis factor-alpha, interleukin-6, rats

CLC Number: 

  • R711.71

Table 1

Number and percentage of high quality oocytes"

Group n Number of oocytes Number of high quality oocytes Percentage of high quality oocytes (%)
Sham-operated 15 122 104 85.25%
Control 12 83 61* 73.49%*
Experimental 13 98 84△ 85.71%△

Table 2

Expressions of TNF-α and IL-6 mRNAs in granulosa cells(x±s)"

Group n Expression of TNF-α mRNA Expression of IL-6 mRNA
Sham-operated 15 0.49±0.12 0.52±0.18
Control 12 0.72±0.14* 0.71±0.10*
Experimental 13 0.51±0.22△ 0.59±0.22△

Figure 1

Expressions of TNF-α and IL-6 mRNAs in granulosa cells 1: 1 kb DNA ladder; 2: Sham-operated group; 3: Experimental group; 4: Control group."

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