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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (10): 1029-1033.doi: 10.3736/jcim20081008

• Original Experimental Research • Previous Articles     Next Articles

Effects of Sisheng Decoction on spontaneous activity and serum concentration of malondialdehyde in mice with yin deficiency sydrome

Su-fang Zhang1,2, Zhe Chen1, Bai Li1, Hong-yun Chen1, Chang-quan Ling1, Xin-yu Wen3()   

  1. 1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    2. Department of Integrated Traditional Chinese and Western Medicine, Pulmonary Hospital, Tongji University, Shanghai 200433, China
    3. Department of Biochemistry, Chinese People's Liberation Army General Hospital, Beijing 100088, China
  • Received:2008-03-04 Online:2008-10-20 Published:2008-10-15

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Objective:

To assess the therapeutic effects of Sisheng Decoction, a compound traditional Chinese herbal medicine, on a mouse model of yin deficiency syndrome induced by thyroid hormone, and to make the preliminary study on its mechanisms.


Methods:

Simultaneous modeling and treatment were carried out. Sixty mice were randomly divided into six groups: normal group, yin deficiency model group, low-, medium- and high-dose Sisheng Decoction group and Shengmai oral liquid group. Normal group and yin deficiency model group were administered with double distilled water. Spontaneous activity and serum concentration of malondialdehyde in different groups were detected.


Results: 

The symptoms of yin deficiency syndrome such as xerostomia, dysphoria and fervescence were improved in the Sisheng Decoction groups. Compared with the yin deficiency model group, the spontaneous activity was increased and the serum concentration of malondialdehyde was decreased in the Sisheng Decoction groups (P<0.05). There was no significant difference between the Sisheng Decoction groups and the Shengmai oral liquid group (P<0.05).


Conclusion: 

Medium- or high-dose Sisheng Decoction is effective for nourishing yin, clearing heat, engendering liquid and allaying thirst. The above effects of Sisheng Decoction may be realised by improving the spontaneous activity and resisting oxidative damage.

Key words: Sisheng Decoction, yin deficiency, physical activity, antioxidants, mice

CLC Number: 

  • R730.7

Table 1

Average quantity of food and water of mice in different groups (x±s)"

Group n Average quantity of food (g) Average quantity of water (ml)
Normal 10 67.62±5.09 47.20±5.73
Yin deficiency model 10 70.92±4.76 56.60±6.54*
Shengmai oral liquid 10 65.53±4.39△ 59.10±9.99*
Low-dose Sisheng Decoction 10 56.82±5.35*△▲ 55.30±8.73*
Medium-dose Sisheng Decoction 10 63.47±5.23△ 53.20±11.57
High-dose Sisheng Decoction 10 63.58±4.19△ 52.50±9.65

Table 2

Body weight of mice in different groups (x±s, g)"

Group n Day 0 Day 5 Day 10
Normal 10 22.35±1.60 28.95±2.55 33.43±4.24
Yin deficiency model 10 22.50±1.87 28.19±3.47 31.67±4.56
Shengmai oral liquid 10 22.65±1.33 28.80±2.68 32.35±3.29
Low-dose Sisheng Decoction 10 22.85±1.11 27.87±1.64 31.92±2.90
Medium-dose Sisheng Decoction 10 22.90±1.37 28.03±1.83 31.48±1.87
High-dose Sisheng Decoction 10 23.00±1.73 28.12±1.44 32.48±2.94

Table 3

Rectal temperature of mice in different groups (x±s, ℃)"

Group n Day 0 Day 5 Day 10
Normal 10 36.96±0.18 37.79±0.43 37.13±0.33
Yin deficiency model 10 36.89±0.53 38.23±0.46* 37.99±0.24*
Shengmai oral liquid 10 36.60±0.74 37.88±0.27 37.52±0.46△
Low-dose Sisheng Decoction 10 36.87±0.39 37.45±0.37△▲ 37.48±0.53△
Medium-dose Sisheng Decoction 10 37.16±0.43▲ 37.47±0.45△▲ 37.41±0.53△
High-dose Sisheng Decoction 10 37.07±0.51▲ 37.52±0.35△▲ 37.45±0.23△

Table 4

Spontaneous activity of mice in different groups (x±s)"

Group n Activity frequency
(frequency/5 min)		 Activity time
(s/5 min)		 Average velocity
(mm/s)
Normal 10 74.20±9.43 212.87±31.60 22.58±5.73
Yin deficiency model 10 75.80±6.51 166.95±23.84* 14.56±3.21*
Shengmai oral liquid 10 79.90±8.50 170.07±27.95* 14.64±3.69*
Low-dose Sisheng Decoction 10 76.40±12.39 166.76±29.20* 14.17±3.99*
Medium-dose Sisheng Decoction 10 77.00±7.54 215.45±14.02△▲ 22.42±3.08△▲
High-dose Sisheng Decoction 10 79.60±10.11 208.31±15.08△▲ 20.26±3.31△▲

Table 5

Serum concentration of MDA of mice in different groups (x±s, μmol/L)"

Group n MDA
Normal 10 1.88±0.32
Yin deficiency model 10 2.91±0.84*
Shengmai oral liquid 10 2.09±0.41△△
Low-dose Sisheng Decoction 10 2.30±0.48△
Medium-dose Sisheng Decoction 10 2.05±0.34△△
High-dose Sisheng Decoction 10 2.19±0.4△
[1] Han MQ, Su JM, Huang HY , et al. Prognostic analysis of advanced non small cell lung cancer treated by sequential chemo-radiation therapy combined with traditional Chinese medicine: A report of 54 cases[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(3):195-198
韩明权, 苏晋梅, 黄海茵 , 等. 序贯化、放疗结合中药治疗54例晚期非小细胞肺癌患者的预后分析[J]. 中西医结合学报, 2003,1(3):195-198
[2] Chen Z, Ling CQ, Huang XQ , et al. Clinical efficacy of Si Sheng decoction in the alleviation of the toxic and side effects of transarterial embolization[J]. Di Er Jun Yi Da Xue Xue Bao, 1997,18(3):272-274
doi: 10.1007/BF02951625
陈喆, 凌昌全, 黄雪强 , 等. 四生汤减轻肝动脉化疗栓塞毒副反应的疗效[J]. 第二军医大学学报, 1997,18(3):272-274
doi: 10.1007/BF02951625
[3] Ling CQ, Shi JH, Chen Z , et al. Study on the mechanisms of alleviating toxic and side effects of radiotherapy by "Sisheng" decoction[J]. Anhui Zhong Yi Xue Yuan Xue Bao, 1997,16(5):55-57
凌昌全, 史剑慧, 陈喆 , 等. 四生汤减轻放射反应的作用机制研究[J]. 安徽中医学院学报, 1997,16(5):55-57
[4] Chen Q. The experimental methods of traditional Chinese medicinal pharmacology(2nd ed)[M]. Beijing: People's Medical Publishing House, 2006,( 2nd ed):778-779
陈奇 . 中药药理研究方法学(第2版)[M]. 北京: 人民卫生出版社, 2006,( 第2版):778-779
[5] Fu XL, Fang ZQ . Modeling methods and appraisal of yin-deficiency syndrome in animals[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 2004,18(2):51-54
doi: 10.3969/j.issn.1008-861X.2004.02.019
付晓伶, 方肇勤 . 阴虚证动物模型的造模方法及评析[J]. 上海中医药大学学报, 2004,18(2):51-54
doi: 10.3969/j.issn.1008-861X.2004.02.019
[6] Zhang SF, Wang XC, Chen Z . Clinical research of traditional Chinese medicine in treatment of toxic and adverse reactions of radiotherapy for malignant tumor: current status[J]. Zhong Xi Yi Jie He Xue Bao, 2007,5(4):470-474
张素芳, 王晓翠, 陈喆 . 中医药对恶性肿瘤放疗减毒作用的临床研究现状[J]. 中西医结合学报, 2007,5(4):470-474
[7] Cheng Y, Chen X, Zhu ZA , et al. Effects of Erigeron breviscapus on the activity of ATPase, SOD and the content of MDA in brain mitochondria in post-traumatic rats'brain[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 2004,18(2):58-60
doi: 10.3969/j.issn.1008-861X.2004.02.021
程英, 陈鑫, 朱志安 , 等. 灯盏花素对大鼠脑挫裂伤脑组织线粒体ATP酶活性及SOD和MDA水平的影响[J]. 上海中医药大学学报, 2004,18(2):58-60
doi: 10.3969/j.issn.1008-861X.2004.02.021
[8] Sun CW, Zhong GG, Jiang Y , et al. Study of antioxidant effect of Astragalus polysaccharide[J]. Zhongguo Yao Li Xue Tong Bao, 1996,12(2):161-163
孙成文, 钟国赣, 江岩 , 等. 黄芪多糖抗氧化损伤作用的研究[J]. 中国药理学通报, 1996,12(2):161-163
[9] Wang DQ, Shen WM, Tian YP , et al. The effects of the three components isolated from Astragalus mongholicus Bunge on scavenging free radical[J]. Zhongguo Yao Li Xue Tong Bao, 1994,10(2):129-132
汪德清, 沈文梅, 田亚平 , 等. 黄芪的三种提取成分对氧自由基作用的影响[J]. 中国药理学通报, 1994,10(2):129-132
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