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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (10): 1024-1028.doi: 10.3736/jcim20081007

• Original Experimental Research • Previous Articles     Next Articles

Effects of traditional Chinese herbal medicine on the neurobehavioral manifestations and the activity of dopamine D2 receptor in corpora striatum of rats with levodopa-induced dyskinesias

Can-xing Yuan1(), Qing Ye1, Jie Wang1, Yan Zhang2, Chong-gang Yuan2   

  1. 1. Department of Neurology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Laboratory of Physiology, School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2008-03-14 Online:2008-10-20 Published:2008-10-15

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Objective: 

To explore the effect of traditional Chinese herbal medicine (TCM) for nourishing liver and kidney, clearing meridians and removing toxic substances, on the neurobehavioral manifestations and the activity of the dopamine D2 receptor in rat with levodopa-induced dyskinesias (LID).


Methods: 

The rat model of Parkinson's disease (PD) was established by injecting 6-hydroxydopamine (6-OHDA) into right substantia nigra of brain, then, the model of LID in rat was produced by injecting levodopa (LD) and benserazide for 4 weeks. The rats were divided into normal control group, 4-week LD treated group, 4-week LD plus TCM treated group, 8-week LD treated group, and 8-week LD plus TCM treated group, and the effect of the TCM on neurobehavioral manifestations was observed. The radioligand binding assay (RLBA) and Scatchard drawing were used to measure the maximal binding capacity of receptor (Bmax) and equilibrium dissociation constant (KD) of the dopamine D2 receptor in corpora striatum.


Results: 

Compared with the 4-week LD treated group and 8-week LD treated group, TCM could decrease abnormal involuntary movement scores of the rats with LID; the RLBA revealed that the dopamine D2 receptor Bmax significantly increased (P<0.05, P<0.01) and the KD significantly decreased (P<0.05).


Conclusion: 

TCM can improve the activity of the dopamine D2 receptor and relieve the symptoms of LID.

Key words: levodopa-induced dyskinesias, traditional Chinese herbal medicine, dopamine D2 receptor, neurobehavioral manifestations, rats

CLC Number: 

  • R747

Figure 1

Effects of TCM on the scores of AIM in rats with LID **P<0.01, vs 0-week; △△P<0.01, vs 8-week LD treated group; ▲▲P<0.01, vs 4-week LD treated group."

Table 1

Effects of TCM on activity of dopamine D2 receptor in rats with LID (x±s)"

Group n Bmax (fmol/mg) KD (nmol/L)
Lesion side Uninjured side Lesion side Uninjured side
Normal control 6 1 286.10±162.43 1 237.36±149.19 1.020±0.126 0.999±0.135
8-week LD treated 6 531.41±51.66** 1 075.24±89.82 1.592±0.064** 1.268±0.083
8-week LD+TCM treated 6 776.54±100.39**△△ 1 204.04±164.41 1.059±0.057△△ 1.075±0.130
4-week LD treated 6 833.01±131.86** 1 138.61±163.94 1.473±0.059** 1.071±0.200
4-week LD+TCM treated 6 976.99±82.25**▲ 1 240.38±159.69 0.898±0.058*▲▲ 0.980±0.157
[1] Ba MW, Liu ZG, Kong M , et al. Establishment and evaluation of levodopa-induced dyskinesias model in rats with Parkinson's disease[J]. Shanghai Jiao Tong Da Xue Xue Bao Yi Xue Ban, 2006,26(7):810
doi: 10.3969/j.issn.1674-8115.2006.07.031
巴茂文, 刘振国, 孔敏 , 等. 左旋多巴诱发帕金森病大鼠异动症模型的建立和评价[J]. 上海交通大学学报 (医学版), 2006,26(7):810
doi: 10.3969/j.issn.1674-8115.2006.07.031
[2] Bao XM, Shu SY. Stereotaxic atlas of rat brain[M]. Beijing: People's Medical Publishing House,1991: 53
包新民, 舒思云 . 大鼠脑立体定位图谱[M]. 北京: 人民卫生出版社,1991: 53
[3] Carman LS, Gage GH, Shults CW . Partial lesion of the substantia nigra: relation between extent of lesion and rotational behavior[J]. Brain Res, 1991,553(2):275-283
doi: 10.1016/0006-8993(91)90835-J
[4] Cao XB, Sun SG, Xu Y , et al. Study of dyskinesia induced by L-dopa in rat model of Parkinson's disease[J]. Zhonghua Yi Xue Za Zhi, 2004,84(6):505-507
曹学兵, 孙圣刚, 徐岩 , 等. 左旋多巴治疗帕金森病诱发异动症的实验研究[J]. 中华医学杂志, 2004,84(6):505-507
[5] Zhang WM, Cai YQ, Xu RX , et al. The activity change of dopamine D2 receptors in caudate-putamen of Parkinson's disease model rats[J]. Zhonghua Shen Jing Wai Ke Ji Bing Yan Jiu Za Zhi, 2002,1(3):266-268
doi: 10.3969/j.issn.1671-2897.2002.03.021
张旺明, 蔡颖谦, 徐如祥 , 等. 帕金森病大鼠模型尾壳核多巴胺D2受体活性变化研究[J]. 中华神经外科疾病研究杂志, 2002,1(3):266-268
doi: 10.3969/j.issn.1671-2897.2002.03.021
[6] Sokoloff P, Schwartz JG . Novel dopamine receptors half a decade later[J]. Trends Pharmacol Sci, 1995,16(8):270-275
doi: 10.1016/S0165-6147(00)89044-6 pmid: 7482988
[7] Nakao N, Shintani-Mizushima A, Kakishita K . The ability of grafted human sympathetic neurons to synthesize and store dopamine: a potential mechanism for the clinical effect of sympathetic neuron autografts in patients with Parkinson's disease[J]. Exp Neurol, 2004,188(1):65-73
doi: 10.1016/j.expneurol.2004.03.004
[8] Brooks DJ, Ibanez V, Sawle GV . Striatal D2 receptor status in patients with Parkinson's disease, striatonigral degeneration, and progressive supranuclear palsy, measured with 11c-raclopride and positron emission tomography[J]. Ann Neurol, 1992,31(2):184-192
doi: 10.1002/(ISSN)1531-8249
[9] Yuan CX, Hu JH . Treatment of Parkinson's disease in 30 cases by nourishing liver and kidneys, unblocking collaterals and relieving toxin[J]. Shanghai Zhong Yi Yao Za Zhi, 2004,38(8):8-9
袁灿兴, 胡建华 . 滋补肝肾、通络解毒法治疗帕金森病30例[J]. 上海中医药杂志, 2004,38(8):8-9
[10] JC, Yuan CX, Li YM , et al.Effect of Chinese herbs to nourish liver and kidney, clear meridians and remove toxic substance on oxidation stress response of rat model with Parkinson's disease[J]. Zhongguo Xin Yao Yu Lin Chuang Za Zhi, 2003,22(3):160-163
何建成, 袁灿兴, 李亚明 , 等. 滋补肝肾通络解毒中药对帕金森病大鼠模型氧化应激反应的影响[J]. 中国新药与临床杂志, 2003,22(3):160-163
[11] He JC, Yuan CX, Wei HC , et al. Effect of integrative Chinese-Western medication on TH and TH mRNA of rats with Parkinson's disease[J]. Zhongguo Lao Nian Xue Za Zhi, 2006,26(1):54-56
何建成, 袁灿兴, 卫洪昌 , 等. 美多巴和中药合用对帕金森病大鼠酪氨酸羟化酶(TH)及TH mRNA的影响[J]. 中国老年学杂志, 2006,26(1):54-56
[12] He JC, Yuan CX, Wei HC , et al. Effect of Chinese herbs to tonify liver and kidney, clear meridians and removing toxic substances on rat cells apoptosis with Parkinson's disease[J]. Zhongguo Lao Nian Xue Za Zhi, 2003,23(2):114-116
何建成, 袁灿兴, 卫洪昌 , 等. 滋补肝肾通络解毒中药对帕金森病大鼠细胞凋亡的影响[J]. 中国老年学杂志, 2003,23(2):114-116
[13] He JC, Yuan CX, Wei HC , et al. Effect of Chinese and Western medicine integration on spinning behavior of rats with Parkinson's disease[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(4):293-295
何建成, 袁灿兴, 卫洪昌 , 等. 中西药合用对帕金森病大鼠旋转行为的影响[J]. 中西医结合学报, 2003,1(4):293-295
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