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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (10): 1045-1048.doi: 10.3736/jcim20081011

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shengqing Capsule on biochemical parameters in mice with cholesterol gallstone

Xue-lin Zhang1, Xiao-qiang Liang2, Hong-gang Gu1, En-wei Ma1, Tian-bi Lin1, Xun Sun1, Jing-zhe Zhang1()   

  1. 1. Department of Hepatobiliary Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-04-10 Online:2008-10-20 Published:2008-10-15

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Objective:

To observe the effects of Shengqing Capsule (SQC), a compound of traditional Chinese herbal medicine, on biochemical parameters in C57BL/6J mice with cholesterol gallstone.


Methods:

Thirty-eight C57BL/6J mice were randomly divided into normal control group (n=10), untreated group (n=15) and SQC group (n=13). Cholesterol gallstone was induced in mice of the latter two groups by feeding high cholesterol diet. Mice in the SQC group were intragastricly administered SQC 0.22 g/(kg·d). After 8-week treatment, animals were sacrificed and sampled to calculate the incidences of stone formation. The contents of serum cholesterols and Ca 2+, and the malonaldehyde (MDA) content and superoxide dismutase (SOD) activity in liver tissues were detected.


Results:

The incidences of stone formation were 73.33% in untreated group, 0% in normal control group, and 23.08% in the SQC group. And the incidence in untreated group was significantly higher than those in the normal control group and the SQC group (P<0.01). Contents of serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in the SQC group were lower than that in the untreated group (P<0.01), and high-density lipoprotein cholesterol (HDL-C) in the SQC group was higher than that in the untreated group (P<0.01). SQC could increase the SOD activity and decrease the MDA content in liver tissues, showing significant differences as compared with those in the untreated group (P<0.05).


Conclusion:

SQC can decrease the incidence of stone formation and improve the biochemical parameters, which may be one of the mechanisms in the treatment and prevention of cholesterol gallstone disease.

Key words: Shengqing Capsule, cholesterol gallstone, mice, cholesterol, malondialdehyde, superoxide dismutase

CLC Number: 

  • R575.6+2

Table 2

Serum TC, LDL-C, HDL-C and Ca2+ contents in three groups (x±s, mmol/L)"

Group n TC LDL-C HDL-C Ca2+
Normal control 10 2.11±0.21** 1.71±0.26** 1.71±0.25** 1.99±0.37
Untreated 15 3.40±0.81 2.51±0.43 0.80±0.23 2.40±0.23
SQC 13 2.24±0.30** 1.32±0.31** 1.65±0.31** 1.89±0.41

Table 2

Activity of SOD and content of MDA in liver tissues in three groups (x±s)"

Group n SOD (U/mg) MDA (nmol/mg)
Normal control 10 118.43±12.27** 1.21±0.36**
Untreated 15 64.03±11.53 3.10±0.22
SQC 13 87.09±10.71* 2.40±0.41*
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