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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (12): 1476-1481.doi: 10.3736/jcim20121222

• Original Experimental Research • Previous Articles     Next Articles

Effects of different extracts of Eugenia caryophyllata on pentylenetetrazole-induced seizures in mice

Hosseini Mahmoud1(),Jafarianheris Taha1,Seddighi Navid1,Parvaneh Mohammad2,Ghorbani Ahmad3,Reza Sadeghnia Hamid2,Rakhshandeh Hassan3   

  1. 1. Neuroscience Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences,Mashhad, Iran
    2. Neuroscience Research Center and Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences,Mashhad, Iran
    3. Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences,Mashhad, Iran
  • Received:2012-07-27 Accepted:2012-10-15 Online:2012-12-20 Published:2018-12-15

Objective: To investigate the possible anticonvulsant effect of different extracts of Eugenia caryophyllata (clove) on pentylenetetrazole (PTZ)-induced seizures in mice.

Methods: The animals were divided into saline, 50, 100, 250 and 500 mg/kg of aqueous extract, 50, 100, 250 and 500 mg/kg of ethanolic extract, and 50, 100, 250 and 500 mg/kg of chloroformic extract of clove groups. The extracts or saline were injected 60 min before each PTZ injection. Latency to the first minimal clonic seizure (MCS) and generalized tonic-clonic seizure (GTCS) and the percent of mortality were recorded.

Results: Aqueous extract of clove at doses of 50, 100, 250 and 500 mg/kg significantly extended the MCS and GTCS latency (P<0.05). The MCS latency in mice treated with 50, 100 and 250 mg/kg of the ethanolic extract was significantly increased (P<0.05). The GTCS latency in mice treated with 50, 100, 250 and 500 mg/kg of ethanolic extract was significantly higher than that of the saline-treated group (P<0.05). There were no significant differences in MCS and GTCS latency between mice treated with different chloroformic extract of clove or saline.

Conclusion: The aqueous and ethanolic extracts of clove could inhibit the PTZ-induced convulsion, and this plant has the potential to be used as a new therapeutic agent for control of seizures. The exact mechanisms and the active compounds that are responsible for the anticonvulsive effect need to be clarified in future studies.

Key words: Eugenia caryophyllata, pentylenetetrazole, seizures, mice


Group n MCS latency GTCS latency
Saline 10 42.40±0.95 50.11±0.87
Aqueous extract of clove (50 mg/kg, i.p.) 10 54.00±2.96* 91.00±10.95**
Aqueous extract of clove (100 mg/kg, i.p.) 10 71.00±8.4* 127.67±17.78**
Aqueous extract of clove (250 mg/kg, i.p.) 10 76.77±12.48* 152.89±29.35**
Aqueous extract of clove (500 mg/kg, i.p.) 10 56.30±5.14* 99.70±15.33**


Group n MCS latency GTCS latency
Saline plus 1% of Tween 80 10 47.18±3.13 63.60±6.94
Ethanolic extract of clove (50 mg/kg, i.p.) 10 61.40±4.84 107.40±12.48△△
Ethanolic extract of clove (100 mg/kg, i.p.) 10 58.83 ±3.10 124.50±12.34△△
Ethanolic extract of clove (250 mg/kg, i.p.) 10 59.87±4.50 96.00±10.63
Ethanolic extract of clove (500 mg/kg, i.p.) 10 53.14±2.26 86.83±5.71
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