SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (10): 1045-1048.doi: 10.3736/jcim20081011

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shengqing Capsule on biochemical parameters in mice with cholesterol gallstone

Xue-lin Zhang1, Xiao-qiang Liang2, Hong-gang Gu1, En-wei Ma1, Tian-bi Lin1, Xun Sun1, Jing-zhe Zhang1()   

  1. 1. Department of Hepatobiliary Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-04-10 Online:2008-10-20 Published:2008-10-15

RichHTML

1

PDF (PC)

55

Like

Objective:

To observe the effects of Shengqing Capsule (SQC), a compound of traditional Chinese herbal medicine, on biochemical parameters in C57BL/6J mice with cholesterol gallstone.


Methods:

Thirty-eight C57BL/6J mice were randomly divided into normal control group (n=10), untreated group (n=15) and SQC group (n=13). Cholesterol gallstone was induced in mice of the latter two groups by feeding high cholesterol diet. Mice in the SQC group were intragastricly administered SQC 0.22 g/(kg·d). After 8-week treatment, animals were sacrificed and sampled to calculate the incidences of stone formation. The contents of serum cholesterols and Ca 2+, and the malonaldehyde (MDA) content and superoxide dismutase (SOD) activity in liver tissues were detected.


Results:

The incidences of stone formation were 73.33% in untreated group, 0% in normal control group, and 23.08% in the SQC group. And the incidence in untreated group was significantly higher than those in the normal control group and the SQC group (P<0.01). Contents of serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in the SQC group were lower than that in the untreated group (P<0.01), and high-density lipoprotein cholesterol (HDL-C) in the SQC group was higher than that in the untreated group (P<0.01). SQC could increase the SOD activity and decrease the MDA content in liver tissues, showing significant differences as compared with those in the untreated group (P<0.05).


Conclusion:

SQC can decrease the incidence of stone formation and improve the biochemical parameters, which may be one of the mechanisms in the treatment and prevention of cholesterol gallstone disease.

Key words: Shengqing Capsule, cholesterol gallstone, mice, cholesterol, malondialdehyde, superoxide dismutase

CLC Number: 

  • R575.6+2

Table 2

Serum TC, LDL-C, HDL-C and Ca2+ contents in three groups (x±s, mmol/L)"

Group n TC LDL-C HDL-C Ca2+
Normal control 10 2.11±0.21** 1.71±0.26** 1.71±0.25** 1.99±0.37
Untreated 15 3.40±0.81 2.51±0.43 0.80±0.23 2.40±0.23
SQC 13 2.24±0.30** 1.32±0.31** 1.65±0.31** 1.89±0.41

Table 2

Activity of SOD and content of MDA in liver tissues in three groups (x±s)"

Group n SOD (U/mg) MDA (nmol/mg)
Normal control 10 118.43±12.27** 1.21±0.36**
Untreated 15 64.03±11.53 3.10±0.22
SQC 13 87.09±10.71* 2.40±0.41*
[1] Zhu PT, Zhang JZ, Cao ZP , et al. Summary of the treatment of 274 patients with chronic biliary tract infection and cholelithiasis[J]. Shanghai Zhong Yi Yao Za Zhi, 1986,20(9):15-17
朱培庭, 张静喆, 曹中平 , 等. 治疗慢性胆道感染、胆石病274例的总结[J]. 上海中医药杂志, 1986,20(9):15-17
[2] Zhu PT, Zhu SM. Practice of traditional Chinese medicine for cholic diseases[M]. Beijing: People's Medical Publishing House,1999: 272
朱培庭, 朱世敏 . 实用中医胆病学[M]. 北京: 人民卫生出版社,1999: 272
[3] Chen Q. The experimental methods of traditional Chinese medicinal pharmacology[M]. Beijing: People's Medical Publishing House,1994: 206-231
陈奇 . 中药药理实验方法学[M]. 北京: 人民卫生出版社,1994: 206-231
[4] Chen XG, Liu JQ, Peng MH , et al. Intrahepatic cholelithiasis in Guangxi area: an analysis of 8 585 cases[J]. Zhonghua Pu Tong Wai Ke Za Zhi, 2002,17(2):99-101
doi: 10.3760/j.issn:1007-631X.2002.02.012
陈希纲, 刘家奇, 彭民浩 , 等. 胆石病临床流行病学调查——附8 585例分析[J]. 中华普通外科杂志, 2002,17(2):99-101
doi: 10.3760/j.issn:1007-631X.2002.02.012
[5] Glass CK, Witztum JL . Atherosclerosis: the road ahead[J]. Cell, 2001,104(4):503-516
doi: 10.1016/S0092-8674(01)00238-0
[6] Huang ZQ . Status quo and future prospects of biliary tract surgery[J]. Zhongguo Shi Yong Wai Ke Za Zhi, 1999,19(1):17-18
黄志强 . 胆道外科的现状与未来[J]. 中国实用外科杂志, 1999,19(1):17-18
[7] Shi JS, Ren Y . Progress of experimental study on biliary tract surgery in China[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 2004,21(12):1418-1419
石景生, 任予 . 我国胆道外科实验研究的进展[J]. 中华实验外科杂志, 2004,21(12):1418-1419
[8] Hou JY. Pharmacology of traditional Chinese medicine[M]. Beijing: China Press of Traditional Chinese Medicine,2002: 120-231
侯家玉 . 中药药理学[M]. 北京: 中国中医药出版社,2002: 120-231
[9] Bie P, Fan YD . Advances in pathogenesis of cholelithiasis[J]. Zhongguo Pu Wai Ji Chu Yu Lin Chuang Za Zhi, 2003,10(5):439-441
别平, 范毓东 . 胆囊结石发病机理的研究进展[J]. 中国普外基础与临床杂志, 2003,10(5):439-441
[10] Geetha A . Evidence for oxidative stress in the gall bladder mucosa of gall stone patients[J]. J Biochem Mol Biol Biophys, 2002,6(6):427-432
doi: 10.1080/1025814021000036179 pmid: 14972799
[11] Abu-Hilal M , McPhail MJ, Marchand L, et al.Malondialdehyde and superoxide dismutase as potential markers of severity in acute pancreatitis[J]. JOP, 2006,7(2):185-192
[12] Xiao ZL, Amaral J, Biancani P . Impaired cytoprotective function of muscle in human gallbladders with cholesterol stones[J]. Am J Physiol Gastrointest Liver Physiol, 2005,288(3):G525-G532
doi: 10.1152/ajpgi.00261.2004 pmid: 15486345
[13] Sipos P, Krisztina H, Blazovics A , et al. Cholecystitis, gallstones and free radical reactions in human gallbladder[J]. Med Sci Monit, 2001,7(1):84-88
pmid: 11208499
[14] Zhang XB, Cui NQ, Li DH . Dynamic experimental study on unconjugated bilirubin, calcium ion and total bile acid contents in pigment gallstone model bile[J]. Zhongguo Zhong Xi Yi Jie He Wai Ke Za Zhi, 2007,13(1):17-20
doi: 10.3969/j.issn.1007-6948.2007.01.006
张西波, 崔乃强, 李东华 . 胆色素结石模型胆汁中UCB、TBA、Ca2+的动态实验研究[J]. 中国中西医结合外科杂志, 2007,13(1):17-20
doi: 10.3969/j.issn.1007-6948.2007.01.006
[15] Pundir CS, Rani K, Garg P , et al. Correlation between chemical composition of biliary calculi and sera of stone formers[J]. Indian J Med Sci, 2002,56(8):373-375
pmid: 12645161
[1] Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[2] Hao Gou, Ruo-chen Huang, Yong-hua Su, Wei Li. Design of dual targeting immunomicelles loaded with bufalin and study of their anti-tumor effect on liver cancer. Journal of Integrative Medicine, 2021, 19(5): 408-417.
[3] Fidele Ntchapda, Fernand C. Tchatchouang, David Miaffo, Barthelemy Maidadi, Lorella Vecchio, Rodrigue E. Talla, Christian Bonabe, Paul F. Seke Etet, Theophile Dimo. Hypolipidemic and anti-atherosclerogenic effects of aqueous extract of Ipomoea batatas leaves in diet-induced hypercholesterolemic rats. Journal of Integrative Medicine, 2021, 19(4): 243-250.
[4] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[5] Morad Hebi, Mohamed Eddouks. Hypolipidemic activity of Tamarix articulata Vahl. in diabetic rats. Journal of Integrative Medicine, 2017, 15(6): 476-482.
[6] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[7] Roslinah Mohamad Hussain, Noor Faradilla Abdullah, Zulkhairi Amom. Killing of Staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity. Journal of Integrative Medicine, 2016, 14(6): 456-464.
[8] Keshav Raj Paudel, Ung-Won Lee, Dong-Wook Kim. Chungtaejeon, a Korean fermented tea, prevents the risk of atherosclerosis in rats fed a high-fat atherogenic diet. Journal of Integrative Medicine, 2016, 14(2): 134-142.
[9] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[10] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[11] Cheng Huang​. Natural modulators of liver X receptors. Journal of Integrative Medicine, 2014, 12(2): 76-85.
[12] Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
[13] Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[14] Md. Khirul Islam, Imran Mahmud, Sanjib Saha, Asit Baron Sarker, Himangsu Mondal, A.S.M. Monjur-Al-Hossain, Md. Anisuzzman. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. Journal of Chinese Integrative Medicine, 2013, 11(5): 343-351.
[15] Sanjib Saha, Faroque Hossain, Md. Anisuzzman, Md. Khirul Islam. Pharmacological evaluation of Musa seminifera Lour. Fruit. Journal of Chinese Integrative Medicine, 2013, 11(4): 253-261.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3