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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (11): 1145-1151.doi: 10.3736/jcim20081108

• Original Experimental Research • Previous Articles     Next Articles

Effects of Qinggan Huoxue Recipe and its separated recipes on the expression of tumor necrosis factor-α in rats with alcoholic liver injury

Tao Wu1,2, Tao Liu1, Pei-yong Zhang1, Lian-jun Xing1, Guang Ji1()   

  1. 1. Institute of Gastroenterology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Research Center of Chinese Medicine Recipe-Syndrome and Systems Biology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2008-07-08 Online:2008-11-20 Published:2008-11-15

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Objective: 

To study the effects of Qinggan Huoxue Recipe (QGHXR), the compound traditional Chinese herbal medicine, and its separated recipes on the expression of tumor necrosis factor-α (TNF-α) mRNA and serum TNF-α content in rats with alcoholic liver injury (ALI).


Methods:

One hundred male Wistar rats were randomly divided into normal control group (n=10), carbon tetrachloride (CCl4) group (n=10) and ALI group (n=80). Rats in the ALI group were intragastrically administered mixed liquor twice a day and intraperitoneally injected with CCl4 twice a week for 6 weeks, rats in the normal control group were intragastrically administered normal saline, and rats in the CCl4 group were intraperitoneally injected with CCl4 and olive oil twice a week continuously. Two rats in the ALI group were sacrificed for histological observation per week. After 4-week modeling, the rats in the ALI group were randomly divided into QGHXR group, Qinggan Recipe (QGR) group, Huoxue Recipe (HXR) group (15 rats in each group), and the others belonged to the untreated group. After 2-week suitable drugs treatment, the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed. Pathological changes in liver tissues were observed by HE staining. The content of plasma TNF-α was assayed by enzyme linked immunosorbent assay, and expression of TNF-α mRNA in the liver tissue was detected by reverse transcription polymerase chain reaction (RT-PCR).


Results: 

QGHXR and its separated recipes improved liver steatosis and inflammation, and in this regard, the QGHXR was superior to the QGR. QGHXR decreased the activity of serum ALT in rats with ALI, but QGR and HXR did not show significant effect in that. The three recipes decreased the activity of AST as compared with the untreated group, but there were no significant differences among the three treated groups. HXR and QGHXR down-regulated the expression of TNF-α mRNA in the liver tissue, but QGR did not show significant effect. HXR and QGHXR also decreased the content of plasma TNF-α, but QGR did not show significant effect in that.


Conclusion:

QGHXR and HXR may provide protection against ALI in rats through decreasing the production of TNF-α.

Key words: Qinggan Huoxue Recipe, Qinggan Recipe, Huoxue Recipe, alcoholic liver disease, tumor necrosis factor-α, rats

CLC Number: 

  • R774.13

Table 1

Effects of QGHXR and its separated recipes on the degree of steatosis and inflammation of livers in rats with ALI (x±s)"

Group n Total score
Normal control 10 0**
CCl4 10 0.10±0.32**▲
Untreated 9 4.56±0.73
QGR 13 2.15±1.28**
HXR 8 1.38±1.06**
QGHXR 9 0.67±0.50**▲

Figure 1

Histopathological changes of liver tissue in rats in different groups by HE staining (Light microscopy, ×200) A: Normal control group; B: Untreated group; C: CCl4 group; D: QGR group; E: HXR group; F: QGHXR group."

Table 2

Effects of QGHXR and its separated recipes on serum ALT and AST activities in rats with ALI(x±s, U/L)"

Group n ALT AST
Normal control 10 36.96±5.31** 105.05±33.04*
CCl4 10 43.67±9.02** 150.72±55.98△
Untreated 9 53.07±10.65 192.62±38.43
QGR 13 47.03±6.06 143.00±53.28*
HXR 8 47.45±3.44 129.51±55.36**
QGHXR 9 43.53±6.66** 120.09±31.15**

Figure 2

Effects of QGHXR and its separated recipes on the expression of TNF-α mRNA in rats with ALI (1) RT-PCR spectrum; (2) Ratio of TNF-α mRNA/β-actin mRNA. A: Normal control group; B: CCl4 group; C: Untreated group; D: QGR group; E: HXR group; F: QGHXR group. *P<0.05, **P<0.01, vs untreated group; △P>0.05, vs normal control group. n=3."

Table 3

Effects of QGHXR and its separated recipes on the content of plasma TNF-α in rats with ALI(x±s, ng/L)"

Group n TNF-α
Normal control 10 24.06±2.80**
CCl4 10 25.86±2.11**
Untreated 9 31.98±4.61
QGR 13 28.58±4.99
HXR 8 25.86±5.05**
QGHXR 9 24.99±4.65**
[1] Ji G . Clinical study on treatment of alcoholic liver disease by Qinggan Huoxue Recipe[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(2):103-107, 124
doi: 10.3969/j.issn.1672-397X.2003.09.008
季光 . 清肝活血方治疗酒精性肝病的临床研究[J]. 中西医结合学报, 2003,1(2):103-107, 124
doi: 10.3969/j.issn.1672-397X.2003.09.008
[2] Ji G, Wang M, Zheng PY , et al. Effects of Qinggan Huoxue Recipe and its decomposed formulas on CD14, Toll like receptor 4 and nuclear factor-κB expressed by Kupffer cells[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(5):509-513
季光, 王淼, 郑培永 , 等. 清肝活血方及其拆方对库普弗细胞表达CD14、Toll样受体4及核因子-κB的影响[J]. 中西医结合学报, 2006,4(5):509-513
[3] Wang L, Ji G, Zheng PY , et al. Establishment of a rat model of alcoholic liver fibrosis induced by complex factors[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(3):281-284
王磊, 季光, 郑培永 , 等. 大鼠酒精性肝纤维化复合模型的建立[J]. 中西医结合学报, 2006,4(3):281-284
[4] Zhang DF. Experiments of pharmacology and traditional Chinese medicine pharmacology[M]. Shanghai: Shanghai Scientific and Technical Publishers,2002: 8-10
张大方 . 药理与中药药理实验[M]. 上海: 上海科学技术出版社,2002: 8-10
[5] Diehl AM, Goodman Z, Ishak KG . Alcohollike liver disease in nonalcoholics. A clinical and histologic comparison with alcohol-induced liver injury[J]. Gastroenterology, 1988,95(4):1056-1062
doi: 10.1016/0016-5085(88)90183-7
[6] Knodell RG, Ishak KG, Black WC , et al. Formulation and application of a numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis[J]. Hepatology, 1981,1(5):431-435
doi: 10.1002/(ISSN)1527-3350
[7] Wang TL, Liu X, Zhou YP , et al. A semiquantitative scoring system for assessment of hepatic inflammation and fibrosis in chronic viral hepatitis[J]. Zhonghua Gan Zang Bing Za Zhi, 1998,66(4):195-197
doi: 10.3760/j.issn:1007-3418.1998.04.002
王泰龄, 刘霞, 周元平 , 等. 慢性肝炎炎症活动度及纤维化程度计分方案[J]. 中华肝脏病杂志, 1998,66(4):195-197
doi: 10.3760/j.issn:1007-3418.1998.04.002
[8] Marra F .Chemokines in liver inflammation and fibrosis[J].Front Biosci, 2002, 7: d1899-d1914
doi: 10.1055/s-0030-1255351 pmid: 12161342
[9] Diehl AM .Liver regeneration[J].Front Biosci, 2002, 7: e301-e314
doi: 10.2741/A925
[10] Thurman RG . Mechanisms of hepatic toxicity Ⅱ. Alcoholic liver injury involves activation of Kupffer cells by endotoxin[J]. Am J Physio1, 1998,275(4 Pt 1):G605-G611
[11] Su GL, Rahemtulla A, Thomas P , et al. CD14 and lipopolysaccharide binding protein expression in a rat model of alcoholic liver disease[J]. Am J Pathol, 1998,152(3):841-849
[12] Andus T, Geiger T, Hirano T , et al. Action of recombinant human interleukin 6, interleukin 1 beta and tumor necrosis factor alpha on the mRNA induction of acute-phase proteins[J]. Eur J Immunol, 1988,18(5):739-746
doi: 10.1002/(ISSN)1521-4141
[13] Faouzi S, Lepreux S, Bedin C , et al. Activation of cultured rat hepatic stellate cells by tumoral hepatocytes[J]. Lab Invest, 1999,79(4):485-493
doi: 10.1080/09291010902731213 pmid: 10212001
[14] Maher JJ, Lozier JS, Scott MK . Rat hepatic stellate cells produce cytokine-induced neutrophil chemoattra-ctant in culture and in vivo[J]. Am J Physiol, 1998,275(4 Pt 1):G847-G853
[15] Reeves HL, Friedman SL .Activation of hepatic stellate cells--a key issue in liver fibrosis[J].Front Biosci, 2002, 7: d808-d826
doi: 10.2741/reeves pmid: 11897564
[16] Diehl AM . Tumor necrosis factor and its potential role in insulin resistance and nonalcoholic fatty liver disease[J]. Clin Liver Dis, 2004,8(3):619-638
doi: 10.1016/j.cld.2004.04.012
[17] Bruck R, Shirin H, Hershkoviz R . Analysis of Arg-Gly-Asp mimetics and soluble receptor of tumour necrosis factor as therapeutic modalities for concanavalin A induced hepatitis in mice[J]. Gut, 1997,40(1):133
doi: 10.1136/gut.40.1.133
[18] Czaja MJ, Xu J, Alt E . Prevention of carbon tetrachloride-induced rat liver injury by soluble tumor necrosis factor receptor[J]. Gastroenterology, 1995,108(6):1849-1854
doi: 10.1016/0016-5085(95)90149-3
[19] Zheng XB, Han DW, Xu RL , et al. Radix salviae miltiorrhizae protects experimental acute hepatic injury by inhibiting tumor necrosis factor-α production[J]. Zhongguo Mian Yi Xue Za Zhi., 1997,13(3):157-158
doi: 10.1007/BF02951625
郑晓宾, 韩德五, 许瑞龄 , 等. 丹参防治实验性急性肝损伤机制的研究[J]. 中国免疫学杂志, 1997,13(3):157-158
doi: 10.1007/BF02951625
[20] Xu F, Lu BG, Yao Z , et al. Effects of red sage root and danshensu on the kinetics of cytokines secreted by hepatic macrophages and the possible mechanism[J]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue, 1996,8(5):262-265, 318
许锋, 陆伯刚, 姚智 , 等. 丹参及丹参素对内毒素刺激下肝巨噬细胞分泌细胞因子影响的动态观察[J]. 中国危重病急救医学, 1996,8(5):262-265, 318
[21] Xu YS, Li YY, Wang DH , et al. Clinical and experimental study about the Jiedu Huayu Recipe in treating endotoxemia of seasonal febrile disease[J]. Zhongguo Yi Yao Xue Bao, 1990,5(3):15-19
徐应抒, 李跃英, 王德华 , 等. 解毒化瘀汤治疗温病内毒素血症的临床与实验研究[J]. 中国医药学报, 1990,5(3):15-19
[22] Cheng ZQ, Du J . Experimental research on the relations between acute heat stasis syndrome in seasonal febrile disease and TNF-α, ET-1[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2001,7(11):25-28
程志强, 杜健 . 温病急性热瘀证与TNF-α、ET-1的相关性实验研究[J]. 中国中医基础医学杂志, 2001,7(11):25-28
[23] He SL, Yang XP, Peng ZP , et al. Experimental study of complex Salvia miltiorrhiza injection in treating endotoxin shock[J]. Zhong Yi Za Zhi., 1983,24(8):75
贺石林, 杨锡平, 彭芝配 , 等. 复方丹参液抗内毒素休克的实验研究[J]. 中医杂志, 1983,24(8):75
[24] Xu F, Chen BG, Yao Z , et al. Dynamic observation of the effect of Rhubarb and emodin on the cytokines secreted by Kupffer cells under the endotoxin excitation in rats[J]. Zhongguo Shi Yan Lin Chuang Mian Yi Xue Za Zhi, 1996,8(5):41-43
许锋, 陈伯刚, 姚智 , 等. 大黄、大黄素对内毒素刺激下大鼠肝巨噬细胞分泌细胞因子影响的动态观察[J]. 中国实验临床免疫学杂志, 1996,8(5):41-43
[25] Liu QS, Wang XQ, Lai LQ , et al. Effects of Sanchi on hepatic expression of TNF-α in alcoholic hepatopathy rats[J]. Zhonghua Zhong Yi Yao Xue Kan, 2008,26(2):317-320
doi: 10.3969/j.issn.1673-7717.2008.02.035
刘庆生, 王小奇, 来立群 , 等. 三七对酒精性肝病大鼠肝组织学和TNF-α影响[J]. 中华中医药学刊, 2008,26(2):317-320
doi: 10.3969/j.issn.1673-7717.2008.02.035
[26] Cao SH, Gao HM, Wang YQ , et al. Effect of Shennong 33 on the cytokine of rats with multiple organ dysfunction syndrome[J]. Zhonghua Ji Zhen Yi Xue Za Zhi, 2003,12(2):94-96
doi: 10.3760/j.issn:1671-0282.2003.02.008
曹书华, 高红梅, 王永强 , 等. "神农33号"对多器官功能障碍综合征大鼠细胞因子的影响[J]. 中华急诊医学杂志, 2003,12(2):94-96
doi: 10.3760/j.issn:1671-0282.2003.02.008
[27] Yang HX, Bai JW, Zhang H , et al. Effect of activating microcirculation and removing blood stasis medicinal herbs on the releasing of cytokines in endotoxin shock in rat[J]. Zhongguo Zhong Xi Yi Jie He Ji Jiu Za Zhi, 2001,1(8):6-8
杨海贤, 白景文, 张宏 , 等. 活血化瘀药对内毒素休克大鼠细胞因子释放的影响[J]. 中国中西医结合急救杂志, 2001,1(8):6-8
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