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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (2): 121-124.doi: 10.3736/jcim20090205

• Original Experimental Research • Previous Articles     Next Articles

Effects of a formula of components from Shengmai Powder on glucocorticoid receptor in rats after thermal injury

 Xiang Lu, Bin-bin Cheng , Chang-quan Ling , Xiao-qiang Yue , Bai Li   

  1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2008-08-07 Accepted:2008-10-13 Online:2009-02-20 Published:2009-02-15

Objective

To investigate the effects of a formula of components from Shengmai Powder, a compound traditional Chinese herbal medicine, on glucocorticoid receptor (GR) in rats after thermal injury.
Methods

A total of 32 male SD rats were randomly assigned into normal control group, untreated group, ginsenosides group and components group, with 8 rats in each group. Rats in the normal control group were intragastrically administered with normal saline (NS) at room temperature once daily. Rats in the untreated group were treated with NS before thermal injury, and rats in the components group and ginsenosides group were once daily treated with a mixture of aqueous extracts of Ophiopogonis Japoni, Fructus schizandrae Chinensis and ginsenosides and ginsenosides respectively. Rats were administered for one week. After the last administration, rats in the untreated group and treated groups underwent thermal injury for one hour, and then were sacrificed immediately by decapitation. Blood serum was collected, and the serum corticosterone (CS) and adrenocorticotropic hormone (ACTH) levels were determined with enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) respectively. The liver, lung and kidney homogenates were used to determine the GR binding capacity by radioligand receptor binding assay. The results were analyzed by one point analysis.
Results

GR binding capacities in liver, lung and kidney cytosols in the untreated group were obviously lower than those in the normal control group (P<0.01). The GR binding capacities in the liver and lung cytosols in the components group and the ginsenosides group were significantly higher than those in the untreated group (P<0.01), but no significant difference was found in kidney cytosol. Compared with the ginsenosides group, GR binding capacity in liver cytosol in the components group was increased (P<0.01), but there were no noticeable differences when compared with the GR binding capacities in lung and kidney cytosols. Serum CS and ACTH levels of the normal rats were (66±16) μg/L and (59±18) ng/L respectively. There were significant differences in CS and ACTH levels between the normal control group and the other groups (P<0.01), in which the serum CS and ACTH levels were (113±33) μg/L and (125±20) ng/L, (123±26) μg/L and (110±30) ng/L and (118±17) μg/L and (115±35) ng/L respectively. But there was no significant difference between the untreated group and the other treated groups.
Conclusion

The formula of components from Shengmai Powder can enhance the effect of ginsenosides in up-regulating GR in rats after thermal injury.

Key words: Shengmai powder, Component formula, Qinsenosides, Thermal injury, Glucocorticoid receptor, Rats

Table 1

The changes of GR binding capacities for live, lung and kidney cytosols in rats (x±s, fmol/mg)"

Group n R0
Liver Lung Kidney
Normal control 8 308±44 226±22 124±17
Untreated 8 226±42** 87±9** 75±14**
Ginsenosides 8 288±27△△▲▲ 104±20**△△ 73±11**
Components 8 360±29△△ 130±13**△△ 83±9**

Table 2

The changes of CS and ACTH concentrations in serum in rats (x±s)"

Group n CS (μg/L) ACTH (ng/L)
Normal control 8 66±16 59±18
Untreated 8 113±33** 125±20**
Ginsenosides 8 118±17** 115±35**
Components 8 123±26** 110±30**
[1] Gupta BBP, Lalchhandama K . Molecular mechanisms of glucocorticoid action[J]. Curr Sci, 2002,83(9):1103-1111
[2] Ling CQ, Li M, Zhu DZ, Chen Z, Yu CQ, Huang XQ, Tan JX, Lu JH, Xu RB, Zhao WK . Clinical and experimental studies on the relationship between the weakness of yin and yang with glucocorticoid receptor[J]. Zhejiang Zhong Xi Yi Jie He Za Zhi, 2001,11(9):532-533
凌昌全, 李敏, 朱德增, 陈喆, 俞超芹, 黄雪强, 谭金兴, 卢军华, 徐仁宝, 赵伟康 . 阴阳虚证与糖皮质激素受体关系的临床与实验研究[J]. 浙江中西医结合杂志, 2001,11(9):532-533
[3] Ling CQ, Li M, Tan JX, Lu JH . Experimental study on protective effect of Chinese herbal medicine on glucocorticoid receptor[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1999,19(5):302-303
凌昌全, 李敏, 谭金兴, 卢军华 . 中药对糖皮质激素受体保护作用的实验研究[J]. 中国中西医结合杂志, 1999,19(5):302-303
[4] Li Y . Relationship between glucocorticoid receptor and deficiency-syndrome and the regulation of traditional Chinese medicine[J]. J Chin Integr Med, 2004,2(3):172-174
李勇 . 糖皮质激素受体与虚证的关系及中药的调节[J]. 中西医结合学报, 2004,2(3):172-174
[5] Wang J, Guo LL, Wang YY . Study on combination components and effectiveness of Chinese traditional herbal formulas[J]. Zhongguo Zhong Yao Yan Jiu, 2006,31(1):5-9
王阶, 郭丽丽, 王永炎 . 中药方剂有效成(组)分配伍研究[J]. 中国中药研究, 2006,31(1):5-9
[6] Li M, Ling CQ, Huang XQ, Shen ZL . Effects of ginsenosides extracted from ginseng stem and leaves on glucocorticoid receptor in different viscera in heat-damaged rats[J]. J Chin Integr Med, 2006,4(2):156-159
李敏, 凌昌全, 黄雪强, 沈志雷 . 人参茎叶皂苷对热损伤大鼠不同脏器糖皮质激素受体的影响[J]. 中西医结合学报, 2006,4(2):156-159
[7] Xu KC, Zhang SC . Experimental studies on cytosol GCR in liver cell of hyperthyroid rats with yin-deficiency[J]. Guangzhou Zhong Yi Xue Yuan Xue Bao, 1990,7(3):145-149
徐克川, 张善澂 . 甲亢类阴虚大鼠肝胞液糖皮质激素受体的实验研究[J]. 广州中医学院学报, 1990,7(3):145-149
[8] Ling C, Li Y, Zhu X, Zhang C, Li M . Ginsenosides may reverse the dexamethasone-induced down-regulation of glucocorticoid receptor[J]. Gen Comp Endocrinol, 2005,140(3):203-209
doi: 10.1016/j.ygcen.2004.11.003
[9] Ling CQ, Chen Z, Yu CQ, Tan JX . Experimental observation on leukocytic glucocorticoid receptor in peripheral blood in 52 cases of yin- and yang-deficiency[J]. Zhong Yi Za Zhi, 1998,39(10):619-620
凌昌全, 陈哲, 俞超芹, 谭金兴 . 52例阴虚阳虚患者外周血混合白细胞糖皮质激素受体的实验观察[J]. 中医杂志, 1998,39(10):619-620
[10] Ling CQ, Li Y . Glucocorticoid receptor: one of deficiency-syndrome relevant proteins?[J]. Shanghai Zhong Yi Yao Za Zhi, 2003,37(5):8-10
凌昌全, 李勇 . 糖皮质激素受体——虚证的相关蛋白之一?[J]. 上海中医药杂志, 2003,37(5):8-10
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