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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (2): 125-129.doi: 10.3736/jcim20090206

• Original Experimental Research • Previous Articles     Next Articles

Effects of Feiyanning formula on expressions of mRNAs and proteins of CXCL12 and CXCR4 in mice with Lewis tumors

 Ji Wua, Bing Baia, Zhen-ye Xub, Zhong-qi Wanga, Xiao-zhen Zhaoa   

  1. a Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b Cancer Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-06-18 Accepted:2008-08-26 Online:2009-02-20 Published:2009-02-15

Objective

To observe the effects of Feiyanning (FYN) formula, a compound traditional Chinese herbal medicine, on the expressions of CXCL12 and CXC chemokine receptor 4 (CXCR4) mRNAs and proteins in Lewis tumors in C57 mice.
Methods

A total of 24 C57BL-6 mice were used in this study. Lewis cells were subcutaneously injected to the right armpit to establish the tumor-bearing model. The C57 mice bearing Lewis tumor were randomly divided into untreated group, chemotherapy group, FYN group and chemotherapy plus FYN group. Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were employed to measure the expressions of CXCL12 and CXCR4 mRNAs and proteins in Lewis tumors in C57 mice respectively.
Results

The rates of lung cancer metastasis in the FYN group and the chemotherapy plus FYN group were markedly lower than that in the untreated group or the chemotherapy group. The expressions of CXCL12 and CXCR4 mRNAs and proteins appeared in tumor tissue in the untreated group. Although there was no significant difference in the expressions of mRNA and protein of CXCL12 among all groups (P>0.05), the expressions of mRNA and protein of CXCR4 in the untreated group and the chemotherapy group were markedly lower than those in the FYN group and the chemotherapy plus FYN group (P<0.05).
Conclusion

FYN can inhibit the metastasis of Lewis tumor loaded in C57 mice. Its mechanisms may be related to its down-regulation of the expression of chemokine receptor CXCR4, and affecting the role of CXCL12-CXCR4 biological axis.

Key words: Feiyanning, Lewis lung carcinoma, CXCR4 receptors, CXC chemokines, Mice

Table 1

Primer sequence"

Gene Forward primer Reverse primer Size (bp)
GAPDH 5'-ACA GCC GCA TCT TCT TGT GCA GTG-3' 5'-GGC CTT GAC TGT GCC GTT GAA TTT-3' 174
CXCL12 5'-GTC AGC CTG AGC TAC CGA-3' 5'-GAA GGG CAC AGT TTG GAG-3' 103
CXCR4 5'-GGC TGA CCT CCT CTT TGT-3' 5'-GTT TCC TTG GCC TTT GAC-3' 203

Table 2

Expressions of CXCL12 and CXCR4 mRNAs in tumor tissues of mice in different groups (x±s)"

Group n Ratio of CXCL12
mRNA/GAPDH
Ratio of CXCR4
mRNA/GAPDH
Untreated 6 0.97±0.37 1.41±0.67
Chemotherapy 6 1.31±0.58 1.34±0.47
FYN 6 1.21±0.54 0.29±0.17*
FYN plus chemotherapy 6 1.23±0.42 0.35±0.18*

Figure 1

PCR electropherogram of expressions of CXCL12 and CXCR4 mRNAs A: Untreated group; B: FYN group; C: FYN plus chemotherapy group; D: Chemotherapy group."

Table 3

Expressions of CXCL12 and CXCR4 proteins in tumor tissues of mice in different groups (x±s)"

Group n CXCL12 (×10–4) CXCR4(×10–2)
Untreated 6 6.07±3.11 5.01±2.45
Chemotherapy 6 5.98±3.04 4.60±2.37
FYN 6 5.98±3.23 2.30±0.99*
FYN plus chemotherapy 6 5.94±2.34 2.50±0.82*
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