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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (2): 140-144.doi: 10.3736/jcim20090209

• Original Experimental Research • Previous Articles     Next Articles

Effects of salvianolate on cardiomyocytic apoptosis and heart function in a swine model of acute myocardial infarction

 Ming-wei Wang, Dian-fu Zhang , Jian-jin Tang, Bo Chen , Lian-sheng Wang , Zhi-jian Yang, Jun Huang   

  1. Department of Cardiology, the First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China
  • Received:2008-04-30 Accepted:2008-10-29 Online:2009-02-20 Published:2009-02-15

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Objective

To observe the effects of salvianolate on cardiomyocytic apoptosis and heart function in a swine model of acute myocardial infarction (AMI).
Methods

A total of 21 young swines were randomly divided into untreated group, low-dose salvianolate (LS) group and high-dose salvianolate (HS) group (7 in each group). AMI was induced by ligaturing left anterior descending coronary artery. After the operation, 400 or 200 mg salvianolate dissolved in 250 mL 5% glucose saline was administered by intravenous drip to swines in the HS group and the LS group respectively for 7 days. The swines in the untreated group were administered with 250 mL 5% glucose saline. The left ventricular ejection fraction (LVEF) and myocardial perfusion were measured by gated myocardial perfusion imaging at the end of the 4th week after operation. And myocardial apoptosis was detected by TdT-mediated dUTP-biotin nick end labeling (TUNEL) method. Apoptosis index was calculated under an optical microscope.
Results

Myocardial apoptotic index in the edge of myocardial infarction was decreased in the HS group and the LS group, which in the HS group was lower than that in the untreated group (P<0.05). Radioactive defect regions were found by gated myocardial perfusion imaging in all the three groups, but those in the HS group and the LS group were less than those in the untreated group (P<0.05). And the levels of myocardial perfusion and LVEF in the HS group were significantly higher than those in the untreated group (P<0.05).
Conclusion

Salvianolate administered by intravenous drip can inhibit the cardiomyocytic apoptosis and improve the function of heart after AMI in swines.

Key words: Myocardial infarction, Salvianolate, Apoptosis, Swines

Figure 1

Myocardial apoptosis in swines with AMI detected by TUNEL (Light microscopy, ×400) A: Untreated group; B: Low-dose salvianolate group; C: High-dose salvianolate group."

Figure 2

Myocardial apoptotic index in swines with AMI in different groups 4 weeks after operation A: Untreated group; B: Low-dose salvianolate group; C: High-dose salvianolate group. *P<0.05, vs untreated group. n=6."

Table 1

Myocardial perfusion scores and left ventricular ejection fractions of swines with AIM in different groups 4 weeks after operation (x±s)"

Group n Myocardial
perfusion		 Left ventricular
ejection fraction (%)
Untreated 6 14.60±0.55 38.50±4.67
Low-dose salvianolate 6 15.20±0.84 43.16±4.33
High-dose salvianolate 6 16.35±0.22* 60.66±5.81*

Figure 3

Gated myocardial perfusion imaging of swines in different groups 4 weeks after operation A: Untreated group; B: Low-dose salvianolate group; C: High-dose salvianolate group. Red color in the picture refers to the normal myocardial perfusion in myocardium. Yellow color in the picture refers to the sparse myocardial perfusion in myocardium. No color/defects refers to no blood perfusion in myocardium."

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