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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (7): 775-782.doi: 10.3736/jcim20110712

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicines Shengmai injection and Xuesaitong injection on ventricular fibrillation threshold and connexin 43 expression in rats with myocardial infarction

Ai-ming Wu1,2, Dong-mei Zhang1,2, Li-xia Lou1,2, Jian-ying Zhai3, Xi-ying Lü1,2, Li-min Chai1,2, Shuo-ren Wang1,2()   

  1. 1. Key Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing City, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
    2. Key Laboratory of Heart Diseases of State Administration of TCM of the People’s Republic of China, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
    3. National Engineering Research Center for R&-D of TCM Multi-ingredient Drugs, Beijing 100079, China
  • Received:2011-02-10 Accepted:2011-03-10 Online:2011-07-20 Published:2011-06-15

Objective: To explore the effects of Shengmai injection and Xuesaitong injection, compound Chinese herbal medicines for replenishing qi and activating blood, on ventricular fibrillation threshold, heart structure and connexin 43 (Cx43) expression in rats with myocardial infarction (MI).
Methods: One hundred male SD rats were randomly divided into sham operation group, model group, Yiqi Huoxue (YQHX) group (Shengmai injection plus Xuesaitong injection) and captopril group. MI model of rats was established by ligating left anterior descending coronary artery, and rats in sham operation group were prepared in the same way except for the ligation of coronary artery. Rats were treated with corresponding drugs for 1 month from next day after modeling. After treatment ventricular fibrillation threshold was detected, and heart weight index, left ventricular internal diameter and percentage of myocardial infarction were measured. Expression of Cx43 mRNA in myocardium was detected by real-time fluorescent quantitative polymerase chain reaction, and expression of Cx43 protein was observed by immunohistochemical method.
Results: Compared with the sham operation group, ventricular fibrillation threshold decreased significantly, heart weight index and left ventricular internal diameter increased, while expressions of Cx43 mRNA and protein decreased remarkably in the model group (P<0.01). Compared with the model group, ventricular fibrillation threshold was increased significantly, heart weight index, left ventricular internal diameter and percentage of myocardial infarction were decreased significantly in the YQHX group and captopril group (P<0.05 or P<0.01). When it comes to expression of Cx43, both Cx43 mRNA and protein expressions were increased remarkably in the YQHX group compared with the model group (P<0.05 or P<0.01), while only density mean and integral optical density of Cx43 protein expression were increased significantly in the captopril group (P<0.05). The enhancements on Cx43 mRNA and positive area sum of Cx43 protein induced by YQHX drugs were stronger than those induced by captopril (P<0.05).
Conclusion: Shengmai injection and Xuesaitong injection have beneficial effects on ventricular fibrillation threshold in rats with MI. The mechanism is related with improving heart structure and reducing Cx43 expression after MI.

Key words: Shengmai injection, Xuesaitong injection, myocardial infarction, ventricular fibrillation threshold, connexin 43, ventricular remodeling, rats

Table 1

Comparison of ventricular fibrillation threshold caused by electric stimulation of left ventricle in vivo and heart weight index among different groups ($\bar{x}$±s)"

Group n Ventricular fibrillation threshold (V) Heart weight index (mg/g)
Sham operation 7 10.14±3.08 3.42±0.32
Model 8 4.63±1.19** 4.11±0.52**
Captopril 7 9.00±3.11△△ 3.56±0.23△△
YQHX 6 11.00±3.69△△ 3.69±0.20

Table 2

Comparison of left ventricular internal diameter and percentage of myocardial infarction among different groups ($\bar{x}$±s)"

Group n Left ventricular internal diameter (mm) Percentage of myocardial infarction (%)
Sham operation 9 3.33±0.29 -
Model 9 5.98±1.28** 41.87±12.32
Captopril 9 4.50±0.83**△△ 25.23±10.29△△
YQHX 9 4.51±0.83**△△ 25.21±11.26△△

Table 3

Comparison of myocardial connexin 43 mRNA expression among different groups ($\bar{x}$±s)"

Group n Connexin 43 mRNA fold value
Sham operation 7 1.03±0.28
Model 5 0.61±0.26**
Captopril 8 0.68±0.14*
YQHX 8 0.99±0.27△▲

Figure 1

Expression of connexin 43 protein in myocardial tissues of rats observed by immunohistochemical method with diaminobenzidine staining in different groups (Light microscopy, ×400) A: Negative control; B: Sham operation group; C: Model group; D: Captopril group; E: Yiqi Huoxue group."

Table 4

Expression of connexin 43 protein in myocardial tissues of rats detected by immunohistochemical staining among different groups ($\bar{x}$±s)"

Group n Density mean Area sum (μm2) IOD mean (μm2)
Sham operation 9 0.273±0.027 2 998.36±428.65 1.70±0.43
Model 9 0.237±0.015** 1 632.21±322.50** 0.81±0.17**
Captopril 9 0.266±0.015 2 076.30±736.57** 1.18±0.24*
YQHX 9 0.270±0.033△△ 2 637.58±607.03△△▲ 1.42±0.13△△
[1] St John Sutton M, Lee D, Rouleau JL, Goldman S, Plappert T, Braunwald E, Pfeffer MA . Left ventricular remodeling and ventricular arrhythmias after myocardial infarction[J]. Circulation, 2003,107(20):2577-2582
doi: 10.1016/j.accreview.2003.08.007 pmid: 12732606
[2] Gutstein DE, Morley GE, Tamaddon H, Vaidya D, Schneider MD, Chen J, Chien KR, Stuhlmann H, Fishman GI . Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin43[J]. Circ Res, 2001,88(3):333-339
doi: 10.1161/01.RES.88.3.333
[3] Cao KJ . Modern advances in the treatment of arrhythmia(Part 3) ventricular arrhythmia treatment strategies(Continued 2)[J]. Zhongguo Xun Huan Za Zhi, 2009,24(2):83-84
曹克将 . 心律失常治疗的现代进展(3)室性心律失常的治疗策略(续2)[J]. 中国循环杂志, 2009,24(2):83-84
[4] Li M, Wang SR, Zhao MJ, Lü XY . Effects of the activating blood, nourshing qi prescription on myocardial apoptosis of heart failure rats after myocardial infarction[J]. Zhong Xi Yi Jie He Xin Nao Xue Guan Bing Za Zhi, 2005,3(1):33-35
李敏, 王硕仁, 赵明镜, 吕浠滢 . 活血益气方药对心肌梗死后心力衰竭大鼠心肌细胞凋亡的影响[J]. 中西医结合心脑血管病杂志, 2005,3(1):33-35
[5] Chen JX, Wang SR, Zhu LQ, Zhao MJ, Lü XY, Qin Y, Zhang Z . Study on cardiac gene expression microarrary and recipe for activating blood circulation and supplementing qi on heart of rats with post-infarcion heart failure[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2005,25(1):45-48
doi: 10.7661/CJIM.2005.1.45
陈金星, 王硕仁, 朱陵群, 赵明镜, 吕唏莹, 秦英, 张壮 . 心肌梗死后心力衰竭大鼠心脏基因表达谱及活血益气方药对其心脏的影响[J]. 中国中西医结合杂志, 2005,25(1):45-48
doi: 10.7661/CJIM.2005.1.45
[6] Chai SB, Wang SR, Yao LF, Wu AM . Effects of Shensong Yangxin Capsule on ventricular remodeling of rats after myocardial infarction and action potential of isolated heart[J]. Beijing Zhong Yi Yao, 2009,28(12):967-971
柴松波, 王硕仁, 姚立芳, 吴爱明 . 参松养心胶囊对大鼠心梗后心室重构及其离体心脏动作电位影响的研究[J]. 北京中医药, 2009,28(12):967-971
[7] Wang SR, Wang ZT, Zhao MJ, Li M . The study on creating and evaluating animal model of disease and syndrome for deficiency of heart-qi in traditional Chinese medicine[J]. Zhongguo Shi Yan Dong Wu Xue Bao, 2002,10(1):33-38
王硕仁, 王振涛, 赵明镜, 李敏 . 心气虚病证动物模型及其评价体系的构建[J]. 中国实验动物学报, 2002,10(1):33-38
[8] Takagawa J, Zhang Y, Wong ML, Sievers RE, Kapasi NK, Wang Y, Yeghiazarians Y, Lee RJ, Grossman W, Springer ML . Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area- and length-based approaches[J]. J Appl Physiol, 2007,102(6):2104-2111
doi: 10.1152/japplphysiol.00033.2007
[9] Smyth JW, Hong TT, Gao D, Vogan JM, Jensen BC, Fong TS, Simpson PC, Stainier DY, Chi NC, Shaw RM . Limited forward trafficking of connexin 43 reduces cell-cell coupling in stressed human and mouse myocardium[J]. J Clin Invest, 2010,120(1):266-279
doi: 10.1172/JCI39740
[10] Mayama T, Matsumura K, Lin H, Ogawa K, Imanaga I . Remodelling of cardiac gap junction connexin 43 and arrhythmogenesis[J]. Exp Clin Cardiol, 2007,12(2):67-76
pmid: 18650985
[11] Zhang WZ, Wang FZ . The relationship between myocardial gap junction and arrhythmia[J]. Zhongguo Xun Huan Za Zhi, 2001,16(6):480-481
张卫泽, 王方正 . 心肌细胞缝隙连接与心律失常的关系[J]. 中国循环杂志, 2001,16(6):480-481
[12] Patel PM, Plotnikov A, Kanagaratnam P, Shvilkin A, Sheehan CT, Xiong W, Danilo P Jr, Rosen MR, Peters NS . Altering ventricular activation remodels gap junction distribution in canine heart[J]. J Cardiovasc Electrophysiol, 2001,12(5):570-577
doi: 10.1046/j.1540-8167.2001.00570.x pmid: 11386519
[13] Beardslee MA, Lerner DL, Tadros PN, Laing JG, Beyer EC, Yamada KA, Kléber AG, Schuessler RB, Saffitz JE . Dephosphorylation and intracellular redistribution of ventricular connexin43 during electrical uncoupling induced by ischemia[J]. Circ Res, 2000,87(8):656-662
doi: 10.1161/01.RES.87.8.656
[14] Huang B, Qin D, El-Sherif N . Spatial alterations of Kv channels expression and K + currents in post-MI remodeled rat heart [J]. Cardiovasc Res, 2001,52(2):246-254
doi: 10.1016/S0008-6363(01)00378-9 pmid: 11684072
[15] Qu CF, Ma LK . Ventricular electrical instability following myocardial infarction[J]. Xin Xue Guan Bing Xue Jin Zhan, 2005,26(4):359-362
屈朝法, 马礼坤 . 心肌梗死后心电不稳定及其研究进展[J]. 心血管病学进展, 2005,26(4):359-362
[16] Wang X, Wu Y, Shen B, Guo ZQ, Wang XF, Cui XY, Nong YB, Kuang W . Clinical study of prevention and treatment for reperfusion injury with Shengmai injection after acute myocardial infarction PCI[J]. Xian Dai Zhong Xi Yi Jie He Za Zhi, 2010,19(28):3553-3554, 3561
王轩, 吴旸, 沈彬, 郭自强, 王小斐, 崔小云, 农一兵, 匡武 . 生脉注射液防治急性心肌梗死介入术后再灌注损伤的临床研究[J]. 现代中西医结合杂志, 2010,19(28):3553-3554, 3561
[17] Wang XY, Wang J, Yang G, Liu YM . Effects of Xuesaitong Soft Capsule on hemodynamics and cardiocyte apoptosis of rats after myocardial infarction[J]. J Chin Integr Med, 2010,8(3):269-274
doi: 10.3736/jcim20100312
王学颖, 王阶, 杨戈, 刘咏梅 . 血塞通软胶囊对心肌梗死大鼠血流动力学及心肌细胞凋亡的影响[J]. 中西医结合学报, 2010,8(3):269-274
doi: 10.3736/jcim20100312
[18] Fu JH, Li XZ, Shang XH, Liu JX . Protective effects of saponines of stem and leaf of Panax notoginseng on acute myocardial ischemia in anaesthetic dogs[J]. Zhongguo Zhong Yao Za Zhi, 2006,31(1):62-65
doi: 10.3321/j.issn:1001-5302.2006.01.018
付建华, 李欣志, 尚晓泓, 刘建勋 . 三七茎叶皂苷对麻醉犬急性心肌缺血的保护作用[J]. 中国中药杂志, 2006,31(1):62-65
doi: 10.3321/j.issn:1001-5302.2006.01.018
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