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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (6): 557-562.doi: 10.3736/jcim20090612

• Original Experimental Research • Previous Articles     Next Articles

Protective effect of catechin on renal microvessels in 5/6 nephrectomized rats and its mechanism

Yan Caoa,Xiao-jie Hea,Wei Xiangb,Zhu-wen Yi a   

  1. a Laboratory of Pediatric Nephropathy, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China
    b Department of Pediatrics,People’s Hospital of Hainan Province,Haikou 570311, Hainan Province, China
  • Received:2008-12-30 Accepted:2009-03-02 Online:2009-06-20 Published:2009-06-15

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Objective

To investigate the effects of catechin on angiotensin-converting enzyme (ACE) activity, angiotensin Ⅱ(Ang Ⅱ) content and microvessel density (MVD) in renal tissues of 5/6 nephrectomized rats.
Methods

Sixty SD rats were divided into 3 groups: sham-operated group, untreated group and catechin group. Animal model was reproduced by 5/6 nephrectomy. After 8- and 12-week administration, rats were sacrificed. Renal MVD was measured by immunohistochemical method with CD34 marking. Activities of ACE in plasma and renal cortices were measured by ultraviolet spectrophotometer, and Ang Ⅱ contents in plasma and renal cortices were measured by radioimmunoassay. Glomerular sclerosis index (GSI) and tubule interstitial score (TIS) were calculated by semiquantitative integration with hematoxylin and eosin staining and periodic acid-Schiff staining.
Results

After 8- and 12-week administration, the GSI and TIS in the catechin group were much less than those in the untreated group (P<0.05, P<0.01). MVDs around glomerulus and tubule at the end of the 8th and 12th week in the untreated group and the catechin group were much less than those in the sham-operated group (P<0.01). The ACE activities and Ang Ⅱcontents in plasma and renal cortices in the catechin group were much less than those in the untreated group (P<0.01). By Pearson correlation analysis, we found that MVD had negative correlation with GSI, TIS, Ang Ⅱ content, and ACE activity(P<0.01), however, the ACE activity and Ang Ⅱ content had positive correlation with GSI, TIS (P<0.01).
Conclusion

Catechin can prevent the 5/6 nephrectomized rats from decreasing of MVD and inhibit the progress of glomerular sclerosis and interstitial fibrosis by inhibiting the activity of ACE and reducing the production of Ang Ⅱ.

Key words: Catechin, Microvessel density, Angiotensin II, Angiotensin-converting enzyme, Rats

Figure 1

Pathological changes of renal tissues in rats after 12-week treatment observed by light microscope (HE staining, ×200) A: Sham-operated group; B: Untreated group; C: Catechin group."

Table 1

Pathological changes of renal tissues in rats after 8- and 12-week treatment（x±s）"

Group n GSI TIS
8 weeks 12 weeks 8 weeks 12 weeks
Sham-operated 10 6.24±0.96 11.75±0.49 0.14±0.02 0.19±0.04
Untreated 10 73.56±4.38** 114.72±5.91**▲▲ 3.78±0.17** 5.63±0.25**▲▲
Catechin 10 51.07±3.75**△△ 79.87±4.33**△▲▲ 2.04±0.12**△△ 3.76±0.47**△▲

Figure 2

MVD changes of renal tissues in rats after 12-week treatment observed by light microscope (DAB coloration, ×200) A: Sham-operated group; B: Untreated group; C: Catechin group."

Table 2

MVD changes of renal tissues in rats after 8- and 12-week treatment（x±s, numbers/mm2）"

Group n Renal glomerulus Renal tubule
8 weeks 12 weeks 8 weeks 12 weeks
Sham-operated 10 46.32±2.90 48.84±3.77 700.14±36.02 692.21±25.66
Untreated 10 25.38±1.82** 14.26±1.01**▲▲ 403.78±10.12** 335.23±15.25**▲▲
Catechin 10 36.28±3.62**△△ 24.96±1.18**△△▲▲ 512.80±16.12**△△ 468.76±11.28**△△▲

Table 3

ACE activity and Ang Ⅱ content of plasma in rats after 8- and 12-week treatment （x±s）"

Group n ACE (U/L) Ang Ⅱ(ng/L)
8 weeks 12 weeks 8 weeks 12 weeks
Sham-operated 10 38.25±2.58 42.32±3.09 348.8±13.7 359.6±15.2
Untreated 10 96.54±5.17** 113.56±9.36**▲▲ 764.8±31.2** 868.6±43.5**▲
Catechin 10 68.27±4.63**△△ 85.32±6.95**△△▲▲ 579.8±28.7**△△ 648.8±23.6**△△▲

Table 4

ACE activity and Ang Ⅱ content of renal cortices in rats after 8- and 12-week treatment （x±s）"

Group n ACE (U/mg) Ang Ⅱ(pg/mg)
8 weeks 12 weeks 8 weeks 12 weeks
Sham-operated 10 41.95±3.77 45.84±3.68 208.5±11.2 216.8±9.9
Untreated 10 148.89±8.66** 176.58±7.78** 578.8±23.6** 748.8±35.6**▲
Catechin 10 89.54±4.70**△△ 108.68±7.78**△△ 411.4±20.7**△△ 446.8±23.0**△△
[1] Ohashi R, Shimizu A, Masuda Y, Kitamura H, Ishizaki M, Sugisaki Y, Yamanaka N . Peritubular capillary regression during the progression of experimental obstructive nephropathy[J]. J Am Soc Nephrol, 2002,13(7):1795-1805
doi: 10.1097/01.ASN.0000018408.51388.57
[2] Razzaque MS, Taguchi T . Cellular and molecular events leading to renal tubulointerstitial fibrosis[J]. Med Electron Microsc, 2002,35(2):68-80
doi: 10.1007/s007950200009
[3] Kang DH, Kanellis J, Hugo C, Truong L, Anderson S, Kerjaschki D, Schreiner GF, Johnson RJ . Role of the microvascular endothelium in progressive renal disease[J]. J Am Soc Nephrol, 2002,13(3):806-816
[4] Burns KD . Angiotensin Ⅱand its receptors in the diabetic kidney[J]. Am J Kidney Dis, 2000,36(3):449-467
doi: 10.1053/ajkd.2000.16192
[5] He XJ, Yi ZW, He QN, Dang XQ, Wu XC, Lu XY, Fang JH . Effects of catechin on expressions of ET and NO in rats with nephrotic syndrome[J]. Zhonghua Er Ke Za Zhi, 2002,40(9):550-554
何小解, 易著文, 何庆南, 党西强, 吴小川, 卢向阳, 方建虎 . 儿茶素对肾病大鼠血浆及肾皮质内皮素、一氧化氮表达影响的研究[J]. 中华儿科杂志, 2002,40(9):550-554
[6] Yang F, de Villiers WJ, McClain CJ, Varilek GW . Green tea polyphenols block endotoxin-induced tumor necrosis factor-production and lethality in a murine model[J]. J Nutr, 1998,128(12):2334-2340
doi: 10.1093/jn/128.12.2334
[7] Zhao ZJ, Zhang XX, Shi SZ, Zhang Y, Chen XM, Ye CH, Niu JZ . The mechanism of delaying rat chronic renal failure development by Shenkang injection[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2000,6(10):16-20
赵宗江, 张新雪, 师锁柱, 张颖, 陈香美, 叶传蕙, 牛建昭 . 肾康注射液延缓5/6肾切除大鼠慢性肾功能衰竭进展的机理探讨[J]. 中国中医基础医学杂志, 2000,6(10):16-20
[8] Raij L, Azar S, Keane W . Mesangial immune injury, hypertension, and progressive glomerular damage in Dahl rats[J]. Kidney Int, 1984,26(2):137-143
doi: 10.1038/ki.1984.147
[9] Radford MG Jr, Donadio JV Jr, Bergstralh EJ, Grande JP . Predicting renal outcome in IgA nephropathy[J]. J Am Soc Nephrol, 1997,8(2):199-207
[10] Kang DH, Joly AH, Oh SW, Hugo C, Kerjaschki D, Gordon KL, Mazzali M, Jefferson JA, Hughes J, Madsen KM, Schreiner GF, Johnson RJ . Impaired angiogenesis in the remnant kidney model: I. Potential role of vascular endothelial growth factor and thrombospondin-1[J]. J Am Soc Nephrol, 2001,12(7):1434-1447
[11] Shimizu A, Kitamura H, Masuda Y, Ishizaki M, Sugisaki Y, Yamanaka N . Rare glomerular capillary regeneration and subsequent capillary regression with endothelial cell apoptosis in progressive glomerulonep-hritis[J]. Am J Pathol, 1997,151(5):1231-1239
[12] Kang DH, Johnson RJ . Vascular endothelial growth factor: a new player in the pathogenesis of renal fibrosis[J]. Curr Opin Nephrol Hypertens, 2003,12(1):43-49
doi: 10.1097/00041552-200301000-00008
[13] Navar LG, Nishiyama A . Why are angiotensin concentrations so high in the kidney?[J]. Curr Opin Nephrol Hypertens, 2004,13(1):107-115
doi: 10.1097/00041552-200401000-00015
[14] Crowley SD, Gurley SB, Oliverio MI, Pazmino AK, Griffiths R, Flannery PJ, Spurney RF, Kim HS, Smithies O, Le TH, Coffman TM . Distinct roles for the kidney and systemic tissues in blood pressure regulation by the renin-angiotensin system[J]. J Clin Invest, 2005,115(4):1092-1099
doi: 10.1172/JCI23378
[15] 刘国树 . 高血压病防治[M]. 北京: 金盾出版社,2000: 94-95
Liu GS. Prevention and treatment of hypertension disease[M]. Beijing: Jindun Publishing House,2000: 94-95
[16] Kaschina E, Unger T. Angiotensin AT1/AT2 receptors: regulation, signalling and function[J].Blood Press, 2003,12(2):70-88
doi: 10.1080/08037050310001057
[17] Liu Y, Sun YF, Ma GX, Li J, Meng G, Han L, Liu X, Li MG . Correlating endothelin releasing function of vascular endothelial cells to tea polyphenols and angiotensinⅡ.[J]. Zhonguo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu, 2008,12(2):381-384
刘玉, 孙运峰, 马贵喜, 李靖, 蒙果, 韩磊, 刘昕, 李鸣皋 . 血管内皮细胞内皮素分泌功能与茶多酚及血管紧张素Ⅱ的相关性[J]. 中国组织工程研究与临床康复, 2008,12(2):381-384
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