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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (6): 552-556.doi: 10.3736/jcim20090611

• Original Experimental Research • Previous Articles     Next Articles

Effects of Liangxue Jiedu Decoction in treating psoriasis in a mouse psoriasis model

Min-jie Gu,Shang-pu Gao, Yong-mei Li   

  1. Department of Dermatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-12-29 Accepted:2009-03-16 Online:2009-06-20 Published:2009-06-15


To study the effects of Liangxue Jiedu Decoction, a compound traditional Chinese herbal medicine with the function of blood-cooling and detoxicating, in treating psoriasis in mice and to explore its mechanism.

(1) Sixty mice were randomly divided into Liangxue Jiedu Decoction group, compound Indigo Naturalis capsule group, acitretin capsule group and normal saline group. Another 10 mice were selected as blank control. After 2-week administration, mice were sacrificed to obtain samples. After hematoxylin and eosin (HE) staining, tail scales with granular layers were calculated by an optical microscope. (2) Except for ten mice in blank group, sixty female mice were injected intraperitoneally with diethylstilbestrol once daily. After 3-day injection, mice were randomly divided into four groups and treated as above description. After 2-week treatment, all mice were injected intraperitoneally with colchicine (2 mg/kg), and sacrificed 6 h after the injection. The mitotic rate in virginal epithelium was calculated after HE staining.

Compared with normal saline, Liangxue Jiedu Decoction could significantly inhibit the mitosis of mouse vaginal epithelium (P<0.01) and promote the formation of granular layers in mouse tail-scale epidermis (P<0.01).

The mechanism of Liangxue Jiedu Decoction in treating psoriasis may be related to promoting granular cell growth and inhibiting proliferation of epidermic cells.

Key words: Liangxue Jiedu Decoction, Psoriasis, Mitosis, Mice

Table 1

Effects of Liangxue Jiedu Decoction on formation of granular layers in mouse tail-scale epidermis (x±s, %)"

Group n Granular layer of scale
Blank 10 4.27±0.65
Normal saline 15 4.33±0.62
Acitretin capsule 15 13.53±2.53**
Compound Indigo Naturalis capsule 15 10.80±2.24**△△
Liangxue Jiedu Decoction 15 12.47±1.89**

Figure 1

Granular layers in mouse tail-scale epidermis observed by light microscopy (HE staining, ×400) A: Blank group; B: Normal saline group; C: Acitretin capsule group; D: Compound Indigo Naturalis capsule group; E: Liangxue Jiedu Decoction group."

Figure 2

Mitosis of mouse vaginal epithelium observed by light microscopy (HE staining, ×400) A: Blank group; B: Normal saline group; C: Acitretin capsule group; D: Compound Indigo Naturalis capsule group; E: Liangxue Jiedu Decoction group."

Table 2

Effects of Liangxue Jiedu Decoction on mitosis of mouse vaginal epithelium (x±s, %)"

Group n Epithelial mitosis
Blank 10 8.90±1.87**
Normal saline 15 12.60±1.55
Acitretin capsule 15 6.87±0.92**
Compound Indigo Naturalis capsule 15 7.33±1.40**
Liangxue Jiedu Decoction 15 7.47±1.25**
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